Anti-Cytochrome P450 2E1 antibody (ab28146)
Key features and details
- Rabbit polyclonal to Cytochrome P450 2E1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Rabbit, Human
- Isotype: IgG
Overview
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Product name
Anti-Cytochrome P450 2E1 antibody
See all Cytochrome P450 2E1 primary antibodies -
Description
Rabbit polyclonal to Cytochrome P450 2E1 -
Host species
Rabbit -
Specificity
The antibody detects a ~50-55 kDa protein, corresponding to the apparent molecular mass of cytochrome P450 IIE1 on SDS-PAGE immunoblots. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Rabbit, Human -
Immunogen
Full length native protein (purified) (Rat)
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General notes
The antibody has been shown to inhibit microsomal benzene metabolism
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Primary antibody notes
The antibody has been shown to inhibit microsomal benzene metabolism -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/5000. Predicted molecular weight: 50-55 kDa. This dilution was sufficient for detection of Cytochrome P450 in 20ug of human liver microsomes by colorimetric immunoblot analysis using Goat anti-Rabbit IgG:AP as the secondary antibody.
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ICC/IF |
1/1000.
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Notes |
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WB
1/5000. Predicted molecular weight: 50-55 kDa. This dilution was sufficient for detection of Cytochrome P450 in 20ug of human liver microsomes by colorimetric immunoblot analysis using Goat anti-Rabbit IgG:AP as the secondary antibody. |
ICC/IF
1/1000. |
Target
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Function
Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. -
Sequence similarities
Belongs to the cytochrome P450 family. -
Cellular localization
Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
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Database links
- Entrez Gene: 1571 Human
- Entrez Gene: 13106 Mouse
- Entrez Gene: 25086 Rat
- Omim: 124040 Human
- SwissProt: P05181 Human
- SwissProt: Q05421 Mouse
- SwissProt: P08682 Rabbit
- SwissProt: P05182 Rat
see all -
Alternative names
- 4-nitrophenol 2-hydroxylase antibody
- CP2E1_HUMAN antibody
- CPE1 antibody
see all
Images
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All lanes : Anti-Cytochrome P450 2E1 antibody (ab28146) at 1/1000 dilution
Lane 1 : Molecular weight marker
Lane 2 : Cell lysates prepared from human liver microsomes
Lane 3 : Cell lysates prepared from rat liver microsomes
Lane 4 : Cell lysates prepared from mouse liver microsomes
Lane 5 : Cell lysates prepared from rabbit liver microsomes
Predicted band size: 50-55 kDa -
ICC/IF image of ab28146 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28146, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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All lanes : Anti-Cytochrome P450 2E1 antibody (ab28146) at 1/2500 dilution
All lanes : Tissue lysate prepared from normal murine liver
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG-HRP at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 50-55 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 3 seconds
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (138)
ab28146 has been referenced in 138 publications.
- Hou X et al. Mesencephalic astrocyte-derived neurotrophic factor reprograms macrophages to ameliorate acetaminophen-induced acute liver injury via p38 MAPK pathway. Cell Death Dis 13:100 (2022). PubMed: 35110525
- Wang C et al. Stat4 rs7574865 polymorphism promotes the occurrence and progression of hepatocellular carcinoma via the Stat4/CYP2E1/FGL2 pathway. Cell Death Dis 13:130 (2022). PubMed: 35136014
- Acevedo-Acevedo S et al. LKB1 acts as a critical brake for the glucagon-mediated fasting response. Hepatol Commun 6:1949-1961 (2022). PubMed: 35357082
- You J et al. Hepatic exosomes with declined MiR-27b-3p trigger RIG-I/TBK1 signal pathway in macrophages. Liver Int 42:1676-1691 (2022). PubMed: 35460174
- Zhu L et al. CYP2E1 plays a suppressive role in hepatocellular carcinoma by regulating Wnt/Dvl2/β-catenin signaling. J Transl Med 20:194 (2022). PubMed: 35509083