Anti-Cytochrome P450 2E1 antibody (ab28146)
- Datasheet
- Specific References (39)
- Protocols
Overview
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Product nameAnti-Cytochrome P450 2E1 antibody
See all Cytochrome P450 2E1 primary antibodies -
DescriptionRabbit polyclonal to Cytochrome P450 2E1
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Host speciesRabbit
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SpecificityThe antibody detects a ~50-55 kDa protein, corresponding to the apparent molecular mass of cytochrome P450 IIE1 on SDS-PAGE immunoblots.
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Tested applicationsSuitable for: IHC-Fr, WB, ICC/IF, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Rabbit, Horse, Guinea pig, Dog, Human, Monkey
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Immunogen
Full length native protein (purified) (Rat)
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General notes
The antibody has been shown to inhibit microsomal benzene metabolism
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, PBS, pH 7.2 -
Concentration information loading... -
PurityProtein A purified
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Primary antibody notesThe antibody has been shown to inhibit microsomal benzene metabolism
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab28146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | 1/300. PubMed: 17021035 | |
| WB | 1/5000. Predicted molecular weight: 50-55 kDa. This dilution was sufficient for detection of Cytochrome P450 in 20ug of human liver microsomes by colorimetric immunoblot analysis using Goat anti-Rabbit IgG:AP as the secondary antibody. | |
| ICC/IF | 1/1000. | |
| IHC-P | Use at an assay dependent concentration. PubMed: 24155903 |
Target
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FunctionMetabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms.
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Sequence similaritiesBelongs to the cytochrome P450 family.
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Cellular localizationEndoplasmic reticulum membrane. Microsome membrane.
- Information by UniProt
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Database links
- Entrez Gene: 1571 Human
- Entrez Gene: 13106 Mouse
- Entrez Gene: 25086 Rat
- Omim: 124040 Human
- SwissProt: P05181 Human
- SwissProt: Q05421 Mouse
- SwissProt: P08682 Rabbit
- SwissProt: P05182 Rat
see all -
Alternative names
- 4-nitrophenol 2-hydroxylase antibody
- CP2E1_HUMAN antibody
- CPE1 antibody
see all
Images
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Western blot - Anti-Cytochrome P450 2E1 antibody (ab28146)All lanes : Anti-Cytochrome P450 2E1 antibody (ab28146) at 1/1000 dilution
Lane 1 : Molecular weight marker
Lane 2 : Cell lysates prepared from human liver microsomes
Lane 3 : Cell lysates prepared from rat liver microsomes
Lane 4 : Cell lysates prepared from mouse liver microsomes
Lane 5 : Cell lysates prepared from rabbit liver microsomes
Predicted band size: 50-55 kDa -
Immunohistochemistry (Frozen sections) - Anti-Cytochrome P450 2E1 antibody (ab28146)This image is courtesy of an anonymous Abreviewab28146 staining cells of rat liver sections by IHC-Fr. Cells were fixed in acetone and blocked with 1% BSA for 5 minutes at 25°C prior to incubating with ab28146 diluted 1/300 for 30 minutes at 25°C. An Alexa Fluor® 594 conjugated goat anti-rabbit antibody was used as the secondary.
Double IF labeling with CYP2E1 (Red) and dipeptidyl peptidase IV (Green)
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Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 2E1 antibody (ab28146)ICC/IF image of ab28146 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28146, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Western blot - Anti-Cytochrome P450 2E1 antibody (ab28146)Image courtesy of an anonymous Abreview.All lanes : Anti-Cytochrome P450 2E1 antibody (ab28146) at 1/2500 dilution
All lanes : Tissue lysate prepared from normal murine liver
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG-HRP at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 50-55 kDa
Observed band size: 55 kDa (why is the actual band size different from the predicted?)
Exposure time: 3 seconds
Protocols
References
This product has been referenced in:
- Hao L et al. Mitochondria-targeted ubiquinone (MitoQ) enhances acetaldehyde clearance by reversing alcohol-induced posttranslational modification of aldehyde dehydrogenase 2: A molecular mechanism of protection against alcoholic liver disease. Redox Biol 14:626-636 (2018). WB ; Mouse . Read more (PubMed: 29156373) »
- Donepudi AC et al. Deficiency of cholesterol 7a-hydroxylase in bile acid synthesis exacerbates alcohol-induced liver injury in mice. Hepatol Commun 2:99-112 (2018). Read more (PubMed: 29404516) »
Customer reviews and Q&As
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