Anti-Cytochrome P450 2E1 antibody (ab28146)
Key features and details
- Rabbit polyclonal to Cytochrome P450 2E1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Rabbit, Human
- Isotype: IgG
Overview
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Product name
Anti-Cytochrome P450 2E1 antibody
See all Cytochrome P450 2E1 primary antibodies -
Description
Rabbit polyclonal to Cytochrome P450 2E1 -
Host species
Rabbit -
Specificity
The antibody detects a ~50-55 kDa protein, corresponding to the apparent molecular mass of cytochrome P450 IIE1 on SDS-PAGE immunoblots. -
Tested Applications & Species
Application Species ICC/IF HumanWB MouseRatRabbitHuman -
Immunogen
Full length native protein (purified) (Rat)
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General notes
The antibody has been shown to inhibit microsomal benzene metabolism
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading...
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Purity
Protein A purified -
Primary antibody notes
The antibody has been shown to inhibit microsomal benzene metabolism -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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ICC/IF |
Human
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WB |
Mouse
Rat
Rabbit
Human
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Application | Abreviews | Notes |
---|---|---|
WB | (2) |
1/5000. Predicted molecular weight: 50-55 kDa. This dilution was sufficient for detection of Cytochrome P450 in 20ug of human liver microsomes by colorimetric immunoblot analysis using Goat anti-Rabbit IgG:AP as the secondary antibody.
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ICC/IF |
1/1000.
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Notes |
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WB
1/5000. Predicted molecular weight: 50-55 kDa. This dilution was sufficient for detection of Cytochrome P450 in 20ug of human liver microsomes by colorimetric immunoblot analysis using Goat anti-Rabbit IgG:AP as the secondary antibody. |
ICC/IF
1/1000. |
Target
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Function
Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. -
Sequence similarities
Belongs to the cytochrome P450 family. -
Cellular localization
Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
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Database links
- Entrez Gene: 1571 Human
- Entrez Gene: 13106 Mouse
- Entrez Gene: 25086 Rat
- Omim: 124040 Human
- SwissProt: P05181 Human
- SwissProt: Q05421 Mouse
- SwissProt: P08682 Rabbit
- SwissProt: P05182 Rat
see all -
Alternative names
- 4-nitrophenol 2-hydroxylase antibody
- CP2E1_HUMAN antibody
- CPE1 antibody
see all
Images
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All lanes : Anti-Cytochrome P450 2E1 antibody (ab28146) at 1/1000 dilution
Lane 1 : Molecular weight marker
Lane 2 : Cell lysates prepared from human liver microsomes
Lane 3 : Cell lysates prepared from rat liver microsomes
Lane 4 : Cell lysates prepared from mouse liver microsomes
Lane 5 : Cell lysates prepared from rabbit liver microsomes
Predicted band size: 50-55 kDa -
ICC/IF image of ab28146 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28146, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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All lanes : Anti-Cytochrome P450 2E1 antibody (ab28146) at 1/2500 dilution
All lanes : Tissue lysate prepared from normal murine liver
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG-HRP at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 50-55 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 3 seconds
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (82)
ab28146 has been referenced in 82 publications.
- Wang RY et al. CYP2E1 changes the biological function of gastric cancer cells via the PI3K/Akt/mTOR signaling pathway. Mol Med Rep 21:842-850 (2020). PubMed: 31974627
- Li Y et al. Chlorpromazine protects against acetaminophen-induced liver injury in mice by modulating autophagy and c-Jun N-terminal kinase activation. Liver Res 3:65-74 (2019). PubMed: 31815033
- Yamaguchi T et al. A chronic toxicity study of diphenylarsinic acid in the drinking water of C57BL/6J mice for 52 weeks. J Toxicol Pathol 32:127-134 (2019). PubMed: 31404369
- Zhong F et al. Complement C3 activation regulates the production of tRNA-derived fragments Gly-tRFs and promotes alcohol-induced liver injury and steatosis. Cell Res 29:548-561 (2019). PubMed: 31076642
- Jian T et al. Hepatoprotective Effect of Seed Coat ofEuryale ferox Extract in Non-alcoholic Fatty Liver Disease Induced by High-fat Diet in Mice by Increasing IRs-1 and Inhibiting CYP2E1. J Oleo Sci 68:581-589 (2019). PubMed: 31092797