Anti-Cytochrome P450 2E1 antibody (ab28146)

Thank you for kindly confirming these details as this enables us to closely monitor the quality of our products

We would be pleased to arrange a replacement product free of charge of ab28146 from a different lot. I am forwarding these details on to our Distributor’s Team who would be happy to organize this.

Distributor name: xxxxxxxxxx
Original order number: xxxxxx
abID of original product: 1 X vial ab28146
abID of replacement product: ab28146
Original batch number: xxxxxxx
CCEID: xxxxxxx
New order number: xxxxxxx

Thank you for your help and cooperation. Please do not hesitate to contact us if you need anything further.

Thank you for taking time to answer my questions. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

Having reviewed the protocol details, I believe this product should have given satisfactory results. It appears that you may have received a faulty vial.

I apologize for the inconvenience and am pleased to offer you a free of charge replacement, credit note, or refund in compensation.

If you would like a free of charge replacement, I could either send you the same antibody ab28146 of another lot or, I can suggest two other Anti-Cytochrome P450 2E1 antibodies: ab19140 (https://www.abcam.com/ab19140) or ab118030 (https://www.abcam.com/ab118030). Both of these are guaranteed to work in human in Western blot.

Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

Having reviewed this case, I would like to ask you a few more questions about the protocols used so that I may give you the best possible technical help.

1. How did you prepare your samples? reducing agent used? boiled samples? It might be that you need more sample to get a good band. we usually recommend loading 20-40 ug per well.

2. Did you check the protein transfer with ponceau? If yes, did the relevant band transfer successfully?

3. What blocking solution did you use? If it was 5% milk, you could try switching to 3% BSA for 1 hour at room temperature. If you used 3% BSA, try blocking with milk.

4. How long did you incubate with your primary antibody? we suggest incubating overnight at 4ºC

You could also re-send an image of your blot with a labelled protein ladder and labelled wells so that we can better understand your experiment?

In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

I hope this information is helpful, and I thank you for your cooperation.

Thank you for taking time to answer my questions.



Having reviewed the answers, I would like to offer some suggestions to help optimize the results from ab28146 Anti-Cytochrome P450 2E1 antibody.



1.I can suggest RIPA buffer as lysis buffer as this one would be more suitable for a membrane protein: Please keep in mind that not only you will have to break the tissue down, you also need to dissolve the cell membrane and the ER membrane to dissolve your protein. In addition, I would strongly recommend using protease inhibitors to protect your protein and to clear up your blot:it may be an idea to add at leastLeupeptin to prevent lysosomal protease degradation. However, it may be worth to add an all-aroundprotease inhibitor cocktail, as a lot of the sidebands maybe simply there due to protein degradation.

2.We usually recommend using an average of 20-30ug total protein per well in order to avoid the under-loading the Western Blot. This usually results in a higher dilution of the antibody as well.

3. To increase the efficiency of the blocking, please block at room temperature for 1h.

4. To decrease the side-bands, you could also add 1% milk into your primary antibody dilution.

5. Please perform stringent wash steps of 3x 5 min with TBST after primary and secondary incubation. this can help to clean up the blot.


Should the suggestions not improve the results, please do let me know.

In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

I hope this information is helpful, and I thank you for your cooperation.

Thank you for contacting Abcam’s scientific support team.

A replacement is available within 6 months of purchase if the antibody has failed for applications and species listed on the datasheet.

However, with some details of your western blot protocol, in addition to what you have already told us, we may be able to help you obtain better data more quickly, compared to starting over with a different antibody. The following is a list of basic protocol questions, but if you have any other information that you think is relevant, please include it in your reply.
1. What is the lot number?


2. How do you prepare the samples: what's the lysis buffer? Do you add protease inhibitors?

3. Are you using beta- mercaptoethanol or DTT in your sample buffer? Do you boil your samples? If so, how long and at which temperature?

4. Are you confident the transfer was effective, by validation, for example, with a Ponceau Red stain, or data from other antibodies?


5. What was the membrane blocking reagent? For how long do you block? How long do you block?


6. What were the conditions of the primary antibody incubation: incubation time and temperature? Do you use a blocking agent as well


7. Are you confident the secondary antibody and detection system are effective?


In addition, any images of your data will be helpful. Please attach them to your reply. To replace the antibody, or to issue a credit or refund, we will also need the order details, either the six-digit Abcam order reference number or a purchase order number.


I look forward to your reply.

Thank you for contacting us.

Both mentioned antibodies have been validated to react with rat species. At Abcam, our aim is to share all the information we have available from our products in the Website, to help customers decide the best option for their requirements.

For product ab28146 there are nine references available (under the Tab Specific References) as well as three Abreviews corresponding to customer’s results using this antibody. I hope you find them useful.

Unfortunately, there aren’t any references or Abreviews available for ab22721. As soon as we gather further information or publications referring to our products, we will immediately update our datasheets.

In any case, all of our products are covered by our Abpromise® guarantee, which ensures that you can trust our products, and they should work in the tested species and applications stated on the datasheet, or we will offer a replacement, credit, or refund, if reported within 6 months of purchase.

To read about our Abpromise policy: https://www.abcam.com/index.html?pageconfig=abpromise

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.