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Anti-Cytochrome P450 2E1 antibody (ab28146)

  • Datasheet
  • SDS
Reviews (6)Q&A (6)References (82)

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Western blot - Anti-Cytochrome P450 2E1 antibody (ab28146)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 2E1 antibody (ab28146)
  • Western blot - Anti-Cytochrome P450 2E1 antibody (ab28146)

Key features and details

  • Rabbit polyclonal to Cytochrome P450 2E1
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Rabbit, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Cytochrome P450 2E1 antibody
    See all Cytochrome P450 2E1 primary antibodies
  • Description

    Rabbit polyclonal to Cytochrome P450 2E1
  • Host species

    Rabbit
  • Specificity

    The antibody detects a ~50-55 kDa protein, corresponding to the apparent molecular mass of cytochrome P450 IIE1 on SDS-PAGE immunoblots.
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Mouse
    Rat
    Rabbit
    Human
    See all applications and species data
  • Immunogen

    Full length native protein (purified) (Rat)

  • General notes


    The antibody has been shown to inhibit microsomal benzene metabolism

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Primary antibody notes

    The antibody has been shown to inhibit microsomal benzene metabolism
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cytochromes
    • Signal Transduction
    • Metabolism
    • Drug metabolism
    • Signal Transduction
    • Metabolism
    • Alcohol metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipases
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Drug metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Alcohol metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Cytochromes
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Cytochrome P450 2E1 protein (His tag) (denatured) (ab202203)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab28146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Mouse
Rat
Rabbit
Human
Application Abreviews Notes
WB (2)
1/5000. Predicted molecular weight: 50-55 kDa. This dilution was sufficient for detection of Cytochrome P450 in 20ug of human liver microsomes by colorimetric immunoblot analysis using Goat anti-Rabbit IgG:AP as the secondary antibody.
ICC/IF
1/1000.
Notes
WB
1/5000. Predicted molecular weight: 50-55 kDa. This dilution was sufficient for detection of Cytochrome P450 in 20ug of human liver microsomes by colorimetric immunoblot analysis using Goat anti-Rabbit IgG:AP as the secondary antibody.
ICC/IF
1/1000.

Target

  • Function

    Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms.
  • Sequence similarities

    Belongs to the cytochrome P450 family.
  • Cellular localization

    Endoplasmic reticulum membrane. Microsome membrane.
  • Target information above from: UniProt accession P05181 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1571 Human
    • Entrez Gene: 13106 Mouse
    • Entrez Gene: 25086 Rat
    • Omim: 124040 Human
    • SwissProt: P05181 Human
    • SwissProt: Q05421 Mouse
    • SwissProt: P08682 Rabbit
    • SwissProt: P05182 Rat
    • Unigene: 12907 Human
    • Unigene: 21758 Mouse
    • Unigene: 1372 Rat
    see all
  • Alternative names

    • 4-nitrophenol 2-hydroxylase antibody
    • CP2E1_HUMAN antibody
    • CPE1 antibody
    • CYP2E antibody
    • Cyp2e1 antibody
    • CYPIIE1 antibody
    • Cytochrome P450 2E1 antibody
    • Cytochrome P450 isozyme 3A antibody
    • Cytochrome P450 LM3A antibody
    • Cytochrome P450, family 2, subfamily e, polypeptide 1 antibody
    • cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1 antibody
    • Cytochrome P450, subfamily IIE antibody
    • Cytochrome P450-ALC antibody
    • Cytochrome P450-J antibody
    • Cytochrome P450RLM6 antibody
    • EC 1.14.13.- antibody
    • EC 1.14.13.n7 antibody
    • Ethanol-inducible P450 antibody
    • Flavoprotein-linked monooxygenase antibody
    • MGC133529 antibody
    • Microsomal monooxygenase antibody
    • OTTHUMP00000020813 antibody
    • OTTHUMP00000046571 antibody
    • P450 ALC antibody
    • P450-J antibody
    • P450C2E antibody
    • P450J antibody
    • P450RLM6 antibody
    • Xenobiotic monooxygenase antibody
    see all

Images

  • Western blot - Anti-Cytochrome P450 2E1 antibody (ab28146)
    Western blot - Anti-Cytochrome P450 2E1 antibody (ab28146)
    All lanes : Anti-Cytochrome P450 2E1 antibody (ab28146) at 1/1000 dilution

    Lane 1 : Molecular weight marker
    Lane 2 : Cell lysates prepared from human liver microsomes
    Lane 3 : Cell lysates prepared from rat liver microsomes
    Lane 4 : Cell lysates prepared from mouse liver microsomes
    Lane 5 : Cell lysates prepared from rabbit liver microsomes

    Predicted band size: 50-55 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 2E1 antibody (ab28146)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 2E1 antibody (ab28146)
    ICC/IF image of ab28146 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28146, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Western blot - Anti-Cytochrome P450 2E1 antibody (ab28146)
    Western blot - Anti-Cytochrome P450 2E1 antibody (ab28146)Image courtesy of an anonymous Abreview.
    All lanes : Anti-Cytochrome P450 2E1 antibody (ab28146) at 1/2500 dilution

    All lanes : Tissue lysate prepared from normal murine liver

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG-HRP at 1/5000 dilution

    Developed using the ECL technique.

    Predicted band size: 50-55 kDa
    Observed band size: 55 kDa why is the actual band size different from the predicted?


    Exposure time: 3 seconds

    See Abreview

Protocols

  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (82)

Publishing research using ab28146? Please let us know so that we can cite the reference in this datasheet.

ab28146 has been referenced in 82 publications.

  • Wang RY  et al. CYP2E1 changes the biological function of gastric cancer cells via the PI3K/Akt/mTOR signaling pathway. Mol Med Rep 21:842-850 (2020). PubMed: 31974627
  • Li Y  et al. Chlorpromazine protects against acetaminophen-induced liver injury in mice by modulating autophagy and c-Jun N-terminal kinase activation. Liver Res 3:65-74 (2019). PubMed: 31815033
  • Yamaguchi T  et al. A chronic toxicity study of diphenylarsinic acid in the drinking water of C57BL/6J mice for 52 weeks. J Toxicol Pathol 32:127-134 (2019). PubMed: 31404369
  • Zhong F  et al. Complement C3 activation regulates the production of tRNA-derived fragments Gly-tRFs and promotes alcohol-induced liver injury and steatosis. Cell Res 29:548-561 (2019). PubMed: 31076642
  • Jian T  et al. Hepatoprotective Effect of Seed Coat ofEuryale ferox Extract in Non-alcoholic Fatty Liver Disease Induced by High-fat Diet in Mice by Increasing IRs-1 and Inhibiting CYP2E1. J Oleo Sci 68:581-589 (2019). PubMed: 31092797
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 12 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cytochrome P450 2E1 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH 6.0
Permeabilization
No
Specification
Liver
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Oct 03 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cytochrome P450 2E1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
capybara Tissue sections (Testis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate ph 6
Permeabilization
No
Specification
Testis
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Fixative
Formaldehyde
Read More

Raísa Brito

Verified customer

Submitted Dec 16 2016

Western blot abreview for Anti-Cytochrome P450 2E1 antibody

Excellent
Abreviews
Abreviews
Application
Western blot
Sample
Pig Tissue lysate - other (Adipose tissue)
Loading amount
8 µg
Specification
Adipose tissue
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Dec 18 2012

Question

I have working this antibody for 4 months. I have seriously spent a lot of materials for during experiments. For this reason, I want to you a different lot number for ab28146 and you have suggested other two antibodies. Also, i have spent a lot of SDS-PAGE and Western Blotting materials on the strength of this antibody, i want to these materials would be by you.

Read More

Abcam community

Verified customer

Asked on Nov 23 2012

Answer

Thank you for kindly confirming these details as this enables us to closely monitor the quality of our products

We would be pleased to arrange a replacement product free of charge of ab28146 from a different lot. I am forwarding these details on to our Distributor’s Team who would be happy to organize this.

Distributor name: xxxxxxxxxx
Original order number: xxxxxx
abID of original product: 1 X vial ab28146
abID of replacement product: ab28146
Original batch number: xxxxxxx
CCEID: xxxxxxx
New order number: xxxxxxx

Thank you for your help and cooperation. Please do not hesitate to contact us if you need anything further.

Read More

Abcam Scientific Support

Answered on Nov 23 2012

Question

I use ab28146 rabbit polyclonal to CYP2E1 primary antibody and it's lot number: GR8111-3.First of all, i m studying to CYP2E1 (57 kDa) in C57Bl/6 and Swiss albino mice in Parkinson’s disease.
1. I m preparing %10 gel and using Mini Protean 3. I’m loading a gel liver samples of 10 micrograms and brain samples of 25 micrograms and i using Page Ruler Prestained molecular marker (1 microliter). i prepare samples with sample reducing buffer.
2. I use blocking solution TBS 5% milk.
3. I’m preparing the antibody dilution with TBS-T and i washing the Nitrocellulose membrane with TBS-T. i use 1/1000 primer antibody dilutions.Membrane was incabated primary antibody overnight 4 C. I’m using seconder antibody 1/2000 dilution with alkalen phospatase and membrane visualing with BCIP/NBT premixed solution
4.i m using fermentas prestained protein ladder as a marker.

NC membrane image was attached. Primer antibody didn't binding to CYP2E1 protein, it's connecting to other protein bands (About 70 kDa and 100 kDa have binding). Normally, the spesific CYP2E1 primer antikor must be binding directly CYP2E1 protein band. i didn't get it this points.
(Ladder line; 4. line (red line 70 kDa, 5. Line (blue line 55 kDa) (2 lane brains lane next to protein ladder, the others liver lane)

Read More

Abcam community

Verified customer

Asked on Nov 22 2012

Answer

Thank you for taking time to answer my questions. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

Having reviewed the protocol details, I believe this product should have given satisfactory results. It appears that you may have received a faulty vial.

I apologize for the inconvenience and am pleased to offer you a free of charge replacement, credit note, or refund in compensation.

If you would like a free of charge replacement, I could either send you the same antibody ab28146 of another lot or, I can suggest two other Anti-Cytochrome P450 2E1 antibodies: ab19140 (https://www.abcam.com/ab19140) or ab118030 (https://www.abcam.com/ab118030). Both of these are guaranteed to work in human in Western blot.

Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

Read More

Abcam Scientific Support

Answered on Nov 22 2012

Question

I use ab28146 rabbit polyclonal to CYP2E1 primary antibody and it's lot number: GR8111-3. First of all, i m studying to CYP2E1 (57 kDa) in C57Bl/6 and Swiss albino mice in Parkinson’s disease. I’m investigating this enzyme using by the method Western Blotting. I m preparing %10 gel and using Mini Protean 3. I’m loading a gel liver samples of 10 micrograms and brain samples of 25 micrograms and i using Page Ruler Prestained molecular marker( 1 microliter). Gel running conditions, i have tried 150 V 65 minutes, 150 V 80 minutes and 140 V 100 minutes. Blotting conditions, 70 Volt, 2 hours, + 4 C. I’m using ab28146 Rabbit polyclonal to CYP2E1 primer antibody. I’m preparing the antibody dilution with TBS-T and i washing the Nitrocellulose membrane with TBS-T. So far, i’ve tried 1/1000, 1/5000, 1/10000 and 1/20000 primer antibody dilutions. I’m using seconder antibody (tried to 1/2000 and 1/10000) with alkalen phospatase and membrane visualing with BCIP/NBT premixed solution Despite all conditions, i didn't see CYP2E1 proteins band in Western Blotting.
I see CYP2E1 protein band in SDS-PAGE but i didnt see this bands in Western blotting. I attached my NC membrane (1/5000 primer antibody-1/2000 seconder antibody) and i m using fermentas prestained protein ladder as a marker.
(Ladder line; 4. line 70 kDa, 5. Line 55 kDa) (2 lane brains lane next to protein ladder, the others liver lane)

Read More

Abcam community

Verified customer

Asked on Nov 22 2012

Answer

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

Having reviewed this case, I would like to ask you a few more questions about the protocols used so that I may give you the best possible technical help.

1. How did you prepare your samples? reducing agent used? boiled samples? It might be that you need more sample to get a good band. we usually recommend loading 20-40 ug per well.

2. Did you check the protein transfer with ponceau? If yes, did the relevant band transfer successfully?

3. What blocking solution did you use? If it was 5% milk, you could try switching to 3% BSA for 1 hour at room temperature. If you used 3% BSA, try blocking with milk.

4. How long did you incubate with your primary antibody? we suggest incubating overnight at 4ºC

You could also re-send an image of your blot with a labelled protein ladder and labelled wells so that we can better understand your experiment?

In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

I hope this information is helpful, and I thank you for your cooperation.

Read More

Abcam Scientific Support

Answered on Nov 22 2012

Question

what is the lot number?



This is my antibodyhttps://www.abcam.com/Cytochrome-P450-2E1-antibody-ab28146.html



best regards,


Firstly, thank you for your interest.

1. I use ab28146 rabbit polyclonal to CYP2E1 primary antibody and it's lot number: GR8111-3

2. I prepare the samples with 0,1 M potassium phosphate buffer pH;7,4. I don't use protease inhibitor.

3. I use as a reducing agent, sample reducing buffer (in B-mercaptoethanol). Then i boiling samples 4 minutes (95C).

4. I'm confident transfer was effective since blotting conditions were validated by the use of Ponceau S.

5. NC membrane is blocked with %5 TBS-milk (+4 C, 1 hour).

6. I prepare the primary antibody dilution with TBS-T and primary antibody incubation conditions; +4C, overnight.

7. I prepare the secondary antibody dilution with TBS-T and secondary antibody incubation conditions; +4C, 1 hour. I m confident the secondary antibody was effective. The effectiveness of the conditions, the NC membrane was treated with BCIP/NBT premixed solution. The membrane image is attached.

I look forwad to hearing from you,

Read More

Abcam community

Verified customer

Asked on Oct 04 2012

Answer

Thank you for taking time to answer my questions.



Having reviewed the answers, I would like to offer some suggestions to help optimize the results from ab28146 Anti-Cytochrome P450 2E1 antibody.



1.I can suggest RIPA buffer as lysis buffer as this one would be more suitable for a membrane protein: Please keep in mind that not only you will have to break the tissue down, you also need to dissolve the cell membrane and the ER membrane to dissolve your protein. In addition, I would strongly recommend using protease inhibitors to protect your protein and to clear up your blot:it may be an idea to add at leastLeupeptin to prevent lysosomal protease degradation. However, it may be worth to add an all-aroundprotease inhibitor cocktail, as a lot of the sidebands maybe simply there due to protein degradation.

2.We usually recommend using an average of 20-30ug total protein per well in order to avoid the under-loading the Western Blot. This usually results in a higher dilution of the antibody as well.

3. To increase the efficiency of the blocking, please block at room temperature for 1h.

4. To decrease the side-bands, you could also add 1% milk into your primary antibody dilution.

5. Please perform stringent wash steps of 3x 5 min with TBST after primary and secondary incubation. this can help to clean up the blot.


Should the suggestions not improve the results, please do let me know.

In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

I hope this information is helpful, and I thank you for your cooperation.

Read More

Abcam Scientific Support

Answered on Oct 04 2012

Question

First of all, i m studying to CYP2E1 (57 kDa) in C57Bl/6 and Swiss albino mice in Parkinson’s disease. I’m investigating this enzyme using by the method Western Blotting.

I m preparing %10 gel and using Mini Protean 3.

I’m loading a gel liver samples of 10 micrograms and brain samples of 25 micrograms and i using Page Ruler Prestained molecular marker( 1 microliter).

Gel running conditions, i have tried 150 V 65 minutes, 150 V 80 minutes and 140 V 100 minutes. Blotting conditions, 70 Volt, 2 hours, + 4 C.

I’m using ab28146 Rabbit polyclonal to CYP2E1 primer antibody. I’m preparing the antibody dilution with TBS-T and i washing the Nitrocellulose membrane with TBS-T. So far, i’ve tried 1/500, 1/1000, 1/5000, 1/10000 and 1/20000 primer antibody dilutions. I’m using seconder antibody (tried to 1/2000 and 1/10000) with alkalen phospatase and membrane visualing with BCIP/NBT premixed solution Despite all conditions, i’m unable to delete non-spesific bands on the nitrocellulose membran.

1. How can i get more separate protein bands? (in SDS-PAGE)

2. How can i delete non-spesific bands on nitrocellulose membrane?



I look forwad to hearing from you.


Read More

Abcam community

Verified customer

Asked on Oct 01 2012

Answer

Thank you for contacting Abcam’s scientific support team.

A replacement is available within 6 months of purchase if the antibody has failed for applications and species listed on the datasheet.

However, with some details of your western blot protocol, in addition to what you have already told us, we may be able to help you obtain better data more quickly, compared to starting over with a different antibody. The following is a list of basic protocol questions, but if you have any other information that you think is relevant, please include it in your reply.
1. What is the lot number?


2. How do you prepare the samples: what's the lysis buffer? Do you add protease inhibitors?

3. Are you using beta- mercaptoethanol or DTT in your sample buffer? Do you boil your samples? If so, how long and at which temperature?

4. Are you confident the transfer was effective, by validation, for example, with a Ponceau Red stain, or data from other antibodies?


5. What was the membrane blocking reagent? For how long do you block? How long do you block?


6. What were the conditions of the primary antibody incubation: incubation time and temperature? Do you use a blocking agent as well


7. Are you confident the secondary antibody and detection system are effective?


In addition, any images of your data will be helpful. Please attach them to your reply. To replace the antibody, or to issue a credit or refund, we will also need the order details, either the six-digit Abcam order reference number or a purchase order number.


I look forward to your reply.

Read More

Abcam Scientific Support

Answered on Oct 01 2012

Question

Dear Abcam representative,
I am looking for suitable anti-CYP2B1 and anti-CYP2E1 antibodies (reactivity to rats) for my research purpose. Iam interested toyour products (cat.# ab 22721 and ab28146) for these two enzymes. Would you mindproviding me some information about any lab has already used these products or any article that has mentioned these products? Actually I need some supporting citations if I want topurchase these particularproducts.

Thank you.
Sincerely,

Read More

Abcam community

Verified customer

Asked on Mar 13 2012

Answer

Thank you for contacting us.

Both mentioned antibodies have been validated to react with rat species. At Abcam, our aim is to share all the information we have available from our products in the Website, to help customers decide the best option for their requirements.

For product ab28146 there are nine references available (under the Tab Specific References) as well as three Abreviews corresponding to customer’s results using this antibody. I hope you find them useful.

Unfortunately, there aren’t any references or Abreviews available for ab22721. As soon as we gather further information or publications referring to our products, we will immediately update our datasheets.

In any case, all of our products are covered by our Abpromise® guarantee, which ensures that you can trust our products, and they should work in the tested species and applications stated on the datasheet, or we will offer a replacement, credit, or refund, if reported within 6 months of purchase.

To read about our Abpromise policy: https://www.abcam.com/index.html?pageconfig=abpromise

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Mar 13 2012

Western blot abreview for Anti-Cytochrome P450 2E1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - other (Liver)
Loading amount
10 µg
Specification
Liver
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (12% SDS PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Abcam user community

Verified customer

Submitted Aug 01 2011

1-10 of 12 Abreviews or Q&A

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