Overview

  • Product name
    Anti-Cytochrome P450 2E1 antibody
    See all Cytochrome P450 2E1 primary antibodies
  • Description
    Rabbit polyclonal to Cytochrome P450 2E1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human) expressed in E. coli.

  • Positive control
    • 0.2 µg of recombinant human cytochrome P450 2E1.
  • General notes


    The Cytochrome P450 (P450) superfamily of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as nicotinamide adenine dinucleotide phosphate (NADPH) and P450 reductase. There are over 200 known genes which encode P450s. Epoxygenases are those P450s which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega-hydroxylases are those P450s which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). As well as fatty acid metabolism, P450s also metabolize many drugs and toxins. Cytochrome P450 3A4 is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin.

Properties

Applications

Our Abpromise guarantee covers the use of ab4239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 59 kDa.

Target

  • Function
    Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms.
  • Sequence similarities
    Belongs to the cytochrome P450 family.
  • Cellular localization
    Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4-nitrophenol 2-hydroxylase antibody
    • CP2E1_HUMAN antibody
    • CPE1 antibody
    • CYP2E antibody
    • Cyp2e1 antibody
    • CYPIIE1 antibody
    • Cytochrome P450 2E1 antibody
    • Cytochrome P450 isozyme 3A antibody
    • Cytochrome P450 LM3A antibody
    • Cytochrome P450, family 2, subfamily e, polypeptide 1 antibody
    • cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1 antibody
    • Cytochrome P450, subfamily IIE antibody
    • Cytochrome P450-ALC antibody
    • Cytochrome P450-J antibody
    • Cytochrome P450RLM6 antibody
    • EC 1.14.13.- antibody
    • EC 1.14.13.n7 antibody
    • Ethanol-inducible P450 antibody
    • Flavoprotein-linked monooxygenase antibody
    • MGC133529 antibody
    • Microsomal monooxygenase antibody
    • OTTHUMP00000020813 antibody
    • OTTHUMP00000046571 antibody
    • P450 ALC antibody
    • P450-J antibody
    • P450C2E antibody
    • P450J antibody
    • P450RLM6 antibody
    • Xenobiotic monooxygenase antibody
    see all

References

ab4239 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for your enquiry. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview and in return we will offer you 50 Abpoints which can be redeemed on a number of rewards (a further 100 Abpoints will be offered for an image). Please contact us again if you have any additional questions.

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Question
Answer

This antibody was raised against recombinant full length human cytochrome P450 2E1 expressed in E. coli. Being as it is polyclonal, it will recognise multiple epitopes throughout the whole protein.

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Answer

Thank you for your enquiry. Unfortunately, the originator was unable to provide me with the information you have requested. To our knowledge, cross reactivity with pig has not yet been tested. Should you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher. If you have any more questions, please contact us again.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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