• Product name
    Anti-Cytochrome p450 2J2 antibody
    See all Cytochrome p450 2J2 primary antibodies
  • Description
    Rabbit polyclonal to Cytochrome p450 2J2
  • Host species
  • Tested applications
    Suitable for: ELISA, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human) derived from an internal region of Cytochrome p450 2J2.

  • Positive control
    • Extracts from A549, RAW264.7 and NIH-3T3 cells. Human heart tissue. HepG2 and COS7 cells.



Our Abpromise guarantee covers the use of ab69651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    ELISA: 1/10000.
    ICC/IF: 1/500 - 1/1000.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa). Whole Cell Lysis Buffer (4?) is recommended. For example: Solution Final Concentration
    KCl 50mM
    NP-40 1%
    HEPES (pH7.8) 25mM
    DTT 125uM
    Na3VO4 1mM
    NaF 1mM
    + fresh protease and phosphatase inhibitors

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      This enzyme metabolizes arachidonic acid predominantly via a NADPH-dependent olefin epoxidation to all four regioisomeric cis-epoxyeicosatrienoic acids. One of the predominant enzymes responsible for the epoxidation of endogenous cardiac arachidonic acid pools.
    • Tissue specificity
      Highly expressed in heart, present at lower levels in liver, ileum, jejunum, colon, and kidney.
    • Sequence similarities
      Belongs to the cytochrome P450 family.
    • Cellular localization
      Endoplasmic reticulum membrane. Microsome membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Arachidonic acid epoxygenase antibody
      • CP2J2_HUMAN antibody
      • CPJ2 antibody
      • CYP2J2 antibody
      • CYPIIJ2 antibody
      • Cytochrome P450 2J2 antibody
      • Cytochrome P450 family 2 subfamily J polypeptide 2 antibody
      • Cytochrome P450 subfamily IIJ (arachidonic acid epoxygenase) polypeptide 2 antibody
      • Cytochrome P450 subfamily IIJ polypeptide 2 antibody
      • Flavoprotein linked monooxygenase antibody
      • Microsomal monooxygenase antibody
      • OTTHUMP00000010550 antibody
      see all


    • All lanes : Anti-Cytochrome p450 2J2 antibody (ab69651) at 1/500 dilution

      Lane 1 : Extracts from A549 cells
      Lane 2 : Extracts from RAW264.7 cells
      Lane 3 : Extracts from NIH-3T3 cells
      Lane 4 : Extracts from NIH-3T3 cells with immunising peptide at 5 µg

      Lysates/proteins at 5 µg per lane.

      Predicted band size: 58 kDa
      Observed band size: 58 kDa

    • Immunohistochemistry analysis of paraffin-embedded human heart tissue stained with ab69651 at 1/50 dilution, with and without the immunising peptide.
    • Immunofluorescence analysis of HepG2 cells using ab69651 at 1/500 dilution, with and without the immunising peptide.


    This product has been referenced in:
    • Bystrom J  et al. Inducible CYP2J2 and Its Product 11,12-EET Promotes Bacterial Phagocytosis: A Role for CYP2J2 Deficiency in the Pathogenesis of Crohn's Disease? PLoS One 8:e75107 (2013). Read more (PubMed: 24058654) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Thank you for your reply and for providing the additional information.

    You may want to try loading a whole cell lysate as this was what was tested in our lab. It may be that there is not enough protein present in the microsomal prep alone to ...

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    Thank you for taking time to contact us. I am sorry to hear that this antibody is not providing satisfactory results.

    Did you place this order through Migliore? I see an order from April 2011, so unfortunately this antibody is over a year ol...

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