Product nameAnti-Cytochrome P450 3A4/CYP3A4 antibody
See all Cytochrome P450 3A4/CYP3A4 primary antibodies
DescriptionRabbit polyclonal to Cytochrome P450 3A4/CYP3A4
Tested applicationsSuitable for: Flow Cyt, IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human Cytochrome P450 3A4/CYP3A4 aa 234-264 (internal sequence) conjugated to keyhole limpet haemocyanin.
- Human liver tissue; CEM cells; NCI-H460 cell line lysates.
This product was previously labelled as Cytochrome P450 3A4
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityAmmonium Sulphate Precipitation
Purification notesab135813 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
Our Abpromise guarantee covers the use of ab135813 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/50 - 1/100. Predicted molecular weight: 57 kDa.|
FunctionCytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. The enzyme also hydroxylates etoposide.
Tissue specificityExpressed in prostate and liver. According to some authors, it is not expressed in brain (PubMed:19094056). According to others, weak levels of expression are measured in some brain locations (PubMed:19359404 and PubMed:18545703). Also expressed in epithelium of the small intestine and large intestine, bile duct, nasal mucosa, kidney, adrenal cortex, epithelium of the gastric mucosa with intestinal metaplasia, gallbladder, intercalated ducts of the pancreas, chief cells of the parathyroid and the corpus luteum of the ovary (at protein level).
Sequence similaritiesBelongs to the cytochrome P450 family.
modificationsPolyubiquitinated in the presence of AMFR and UBE2G1 and also STUB1/CHIP and UBE2D1 (in vitro).
Cellular localizationEndoplasmic reticulum membrane. Microsome membrane.
- Information by UniProt
- 1,8-cineole 2-exo-monooxygenase antibody
- Albendazole monooxygenase antibody
- Albendazole sulfoxidase antibody
All lanes : Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab135813) at 1/50 dilution
Lane 1 : NCI-H460 cell line lysates
Lane 2 : NCI-H460 cell line lysates with blocking peptide
Lysates/proteins at 35 µg per lane.
Predicted band size: 57 kDa
Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Human liver tissue labelling Cytochrome P450 3A4/CYP3A4 with ab135813 at 1/10 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.
Flow Cytometric analysis of CEM cells labelling Cytochrome P450 3A4/CYP3A4 with ab135813 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
This product has been referenced in:
- Danoy M et al. Optimized protocol for the hepatic differentiation of induced pluripotent stem cells in a fluidic microenvironment. Biotechnol Bioeng 116:1762-1776 (2019). Read more (PubMed: 30883676) »
- Chang YT et al. Role of CYP3A4 in bone marrow microenvironment-mediated protection of FLT3/ITD AML from tyrosine kinase inhibitors. Blood Adv 3:908-916 (2019). Read more (PubMed: 30898762) »