Key features and details
- Rabbit polyclonal to Cytochrome P450 3A4/CYP3A4
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human, Pig
- Isotype: IgG
Product nameAnti-Cytochrome P450 3A4/CYP3A4 antibody
See all Cytochrome P450 3A4/CYP3A4 primary antibodies
DescriptionRabbit polyclonal to Cytochrome P450 3A4/CYP3A4
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Rat, Human, Pig
Recombinant fragment corresponding to Human Cytochrome P450 3A4/CYP3A4 aa 344-497. Expressed in E.coli.
PPTYDTVLQMEYLDMVVNETLRLFPIAMRLERVCKKDVEINGMFIPKGVV VMIPSYALHRDPKYWTEPEKFLPERFSKKNKDNIDPYIYTPFGSGPRNCI GMRFALMNMKLALIRVLQNFSFKPCKETQIPLKLSLGGLLQPEKPVVLKV ESRD
Database link: P08684
- WB: Recombinant human Cytochrome P450 3A4/CYP3A4 protein; Porcine and rat liver tissue. IHC-P: Human liver tissue.
This product was previously labelled as Cytochrome P450 3A4
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 0.05% Proclin 300, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
Our Abpromise guarantee covers the use of ab231816 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionCytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. The enzyme also hydroxylates etoposide.
Tissue specificityExpressed in prostate and liver. According to some authors, it is not expressed in brain (PubMed:19094056). According to others, weak levels of expression are measured in some brain locations (PubMed:19359404 and PubMed:18545703). Also expressed in epithelium of the small intestine and large intestine, bile duct, nasal mucosa, kidney, adrenal cortex, epithelium of the gastric mucosa with intestinal metaplasia, gallbladder, intercalated ducts of the pancreas, chief cells of the parathyroid and the corpus luteum of the ovary (at protein level).
Sequence similaritiesBelongs to the cytochrome P450 family.
modificationsPolyubiquitinated in the presence of AMFR and UBE2G1 and also STUB1/CHIP and UBE2D1 (in vitro).
Cellular localizationEndoplasmic reticulum membrane. Microsome membrane.
- Information by UniProt
- 1,8-cineole 2-exo-monooxygenase antibody
- Albendazole monooxygenase antibody
- Albendazole sulfoxidase antibody
Recombinant human Cytochrome P450 3A4/CYP3A4
Developed using the ECL technique.
Observed band size: 22 kDa why is the actual band size different from the predicted?
All lanes : Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab231816) at 2 µg/ml
Lane 1 : Porcine liver tissue lysate
Lane 2 : Rat liver tissue lysate
Developed using the ECL technique.
Formalin-fixed, paraffin-embedded human liver tissue stained for Cytochrome P450 3A4/CYP3A4 using ab231816 at 20μg/ml in immunohistochemical analysis. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231816 has not yet been referenced specifically in any publications.