Overview

  • Product name
    Anti-Cytochrome P450 4A antibody
    See all Cytochrome P450 4A primary antibodies
  • Description
    Rabbit polyclonal to Cytochrome P450 4A
  • Host species
    Rabbit
  • Specificity
    The immunogen is homologous to rat cytochrome P450 4A2, 4A10, 4A12 and 4A14. Gene synonyms are Cyp4a11, Cyp4a1, Cyp4a8, and Cyp4a3, respectively. The specificity to these specific forms has not been confirmed experimentally. Please note nomenclature for specific forms may differ from mouse to rat.
  • Tested applications
    Suitable for: IHC-Fr, WB, ICC/IF, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Hamster, Cat, Dog, Human, Pig
  • Immunogen

    Synthetic peptide corresponding to Rat Cytochrome P450 4A aa 431-445. The immunogen is homologous to rat cytochrome P450 4A2, A10, A12 and A14.
    Sequence:

    DPSRFAPDSPRHSHS

Properties

Applications

Our Abpromise guarantee covers the use of ab3573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/100.
WB 1/200 - 1/2000. Detects a band of approximately 50 kDa.

A slightly larger band representing 4A3 is also clearly visible.

ICC/IF 1/20 - 1/200.
IHC-P 1/100 - 1/500.
IP Use at an assay dependent concentration.

Target

  • Function
    Catalyzes the omega- and (omega-1)-hydroxylation of various fatty acids such as laurate, myristate and palmitate. Has little activity toward prostaglandins A1 and E1. Oxidizes arachidonic acid to 20-hydroxyeicosatetraenoic acid (20-HETE).
  • Tissue specificity
    Kidney and liver.
  • Sequence similarities
    Belongs to the cytochrome P450 family.
  • Cellular localization
    Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 20-HETE synthase antibody
    • 20-hydroxyeicosatetraenoic acid synthase antibody
    • CP4AB_HUMAN antibody
    • CYP4A antibody
    • Cyp4a1 antibody
    • Cyp4a10 antibody
    • CYP4A11 antibody
    • Cyp4a14 antibody
    • Cyp4a3 antibody
    • CYP4A7 antibody
    • CYP4AII antibody
    • CYPIVA11 antibody
    • Cytochrome P-450HK-omega antibody
    • Cytochrome P450 4A1 antibody
    • Cytochrome P450 4A10 antibody
    • Cytochrome P450 4A11 antibody
    • Cytochrome P450 4A14 antibody
    • Cytochrome P450 4A2 antibody
    • Cytochrome P450 4A3 antibody
    • Cytochrome P450 4A7 antibody
    • Cytochrome P450HL-omega antibody
    • Fatty acid omega-hydroxylase antibody
    • Lauric acid omega-hydroxylase antibody
    see all

Images

  • ab3573 labelling Cytochrome P450 4A (green) in the cytoplasm and membrane of H-4-II-E cells (right), compared to control (left), by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

  • ab3573 labelling Cytochrome P450 4A (green) in the cytoplasm and membrane of HeLa cells (right), compared to control (left), by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

  • ab3573 labelling Cytochrome P450 4A (green) in the cytoplasm and membrane of PC12 cells (right), compared to control (left), by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

  • ab3573 labelling Cytochrome P450 4A in the cytoplasm of Rat liver tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • ab3573 labelling Cytochrome P450 4A in the cytoplasm of Rat kidney tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • ab3573 labelling Cytochrome P450 4A in the cytoplasm and membrane of Human liver tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

     

References

This product has been referenced in:
  • Drolet B  et al. Altered Protein Expression of Cardiac CYP2J and Hepatic CYP2C, CYP4A, and CYP4F in a Mouse Model of Type II Diabetes-A Link in the Onset and Development of Cardiovascular Disease? Pharmaceutics 9:N/A (2017). Read more (PubMed: 29023376) »
  • Zhu J  et al. Additive Neuroprotection of a 20-HETE Inhibitor with Delayed Therapeutic Hypothermia after Hypoxia-Ischemia in Neonatal Piglets. Dev Neurosci 37:376-89 (2015). Read more (PubMed: 25721266) »
See all 7 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Question
Answer

Thank you for contacting us. For ab23677, LTA4 hydrolase, I suggest either a neutrophil or monocyte lysate. We carry THP1 lysate, ab7913, which is a human monocytic leukemia. For a negative control, LTA4 was detected in epidermal keratinocytes and endothelial cells but not whole skin in the following paper: Leukotriene A4 hydrolase in human skin. J Invest Dermatol. 1994 Feb;102(2):253-7. PMID: 8106755 Our lysate ab30166 may be suitable as a negative control but I cannot guarantee you will not see some reactivity. For the cytochrome P450 enzymes, I suggest liver lysates. A positive control for ab4236 is human liver: ab29889. For ab13513 and ab3573, rat liver lysate ab29413 will be appropriate. I am reluctant to recommend a negative control since information regarding absence of expression is difficult to come by but since CP450 is associated with the ER, cytoplasmic fractions of liver lysates (which do not contain membrane) may be useful. For human liver, this would be ab29875, and for rat liver ab29409. You should also have a look at the references listed on the datasheets to see how other researchers have used the antibodies, though the references for the LTA4 are general and not specific to the antibody. I hope this helps. Please do not hesitate to contact us if you have any other questions.

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Answer

I think you must be interested in ab3573 -- Rabbit polyclonal to Cytochrome P450 4A. If so, all the information you need is on the online datasheet under the "immunogen section". Synthetic peptide D(431 PSRFAPDSPRHSHS(445). The immunizing peptide corresponds to amino acid residues 431-445 from rat cytochrome P450 4A. If this is not the antibody you are interested in, please let me know and I'll do my best to help you.

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Answer

Thank you for your enquiry. Ab3573 detects an ~50 kDa protein representing P450 4A in rat liver extract and also detects, to a lesser extent, isoforms 4A1 and 4A3 (in the same rat liver extract sample, a slightly larger band representing 4A3 is also clearly visible). Thus, on your gel, the smaller band would be cytochrome P450 4A. If you have any more questions, please contact us again.

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