Overview

  • Product name

    Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276]
    See all Cytochrome P450 4A/CYP4A11 primary antibodies
  • Description

    Rabbit monoclonal [EPR8276] to Cytochrome P450 4A/CYP4A11
  • Host species

    Rabbit
  • Specificity

    The immunogen is homologous to human cytochrome P450 4A11 and 4A22. The specificity to these specific forms has not been confirmed experimentally.
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Cytochrome P450 4A/CYP4A11 aa 50-150. The immunogen is homologous to human cytochrome P450 4A11 and 4A22.

  • Positive control

    • Mouse liver, Rat liver, Human fetal kidney and fetal liver cell lysates. Human liver and kidney tissue.
  • General notes

     

     

     This product was previously labelled as Cytochrome P450 4A

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab140635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 59 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      Catalyzes the omega- and (omega-1)-hydroxylation of various fatty acids such as laurate, myristate and palmitate. Has little activity toward prostaglandins A1 and E1. Oxidizes arachidonic acid to 20-hydroxyeicosatetraenoic acid (20-HETE).
    • Tissue specificity

      Kidney and liver.
    • Sequence similarities

      Belongs to the cytochrome P450 family.
    • Cellular localization

      Endoplasmic reticulum membrane. Microsome membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • 20-HETE synthase antibody
      • 20-hydroxyeicosatetraenoic acid synthase antibody
      • CP4AB_HUMAN antibody
      • CYP4A antibody
      • Cyp4a1 antibody
      • Cyp4a10 antibody
      • CYP4A11 antibody
      • Cyp4a14 antibody
      • Cyp4a3 antibody
      • CYP4A7 antibody
      • CYP4AII antibody
      • CYPIVA11 antibody
      • Cytochrome P-450HK-omega antibody
      • Cytochrome P450 4A1 antibody
      • Cytochrome P450 4A10 antibody
      • Cytochrome P450 4A11 antibody
      • Cytochrome P450 4A14 antibody
      • Cytochrome P450 4A2 antibody
      • Cytochrome P450 4A3 antibody
      • Cytochrome P450 4A7 antibody
      • Cytochrome P450HL-omega antibody
      • Fatty acid omega-hydroxylase antibody
      • Lauric acid omega-hydroxylase antibody
      see all

    Images

    • All lanes : Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (ab140635) at 1/1000 dilution

      Lane 1 : Mouse liver lysate
      Lane 2 : Rat liver lysate
      Lane 3 : Human fetal kidney lysate
      Lane 4 : Human fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 59 kDa

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Cytochrome P450 4A/CYP4A11 with ab140635 at 1/50 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling Cytochrome P450 4A/CYP4A11 with ab140635 at 1/50 dilution.

    • Immunohistochemical analysis of paraffin embedded Normal Human Brain tissue using ab140635 showing -ve staining.

    • Immunohistochemical analysis of paraffin embedded Normal Human Breast tissue using ab140635 showing -ve staining.

    • Immunohistochemical analysis of paraffin embedded Normal Human Colon tissue using ab140635 showing -ve staining.

    • Immunohistochemical analysis of paraffin embedded Normal Human Liver tissue using ab140635 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Normal Human Tonsil tissue using ab140635 showing -ve staining.

    References

    This product has been referenced in:

    • Morgan ET  et al. Physiological Regulation of Drug Metabolism and Transport: Pregnancy, Microbiome, Inflammation, Infection, and Fasting. Drug Metab Dispos 46:503-513 (2018). Read more (PubMed: 29514828) »
    • Nyagode BA  et al. Selective effects of a therapeutic protein targeting tumor necrosis factor-alpha on cytochrome P450 regulation during infectious colitis: Implications for disease-dependent drug-drug interactions. Pharmacol Res Perspect 2:e00027 (2014). WB . Read more (PubMed: 24707356) »
    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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