Recombinant Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (ab140635)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8276] to Cytochrome P450 4A/CYP4A11
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276]
See all Cytochrome P450 4A/CYP4A11 primary antibodies -
Description
Rabbit monoclonal [EPR8276] to Cytochrome P450 4A/CYP4A11 -
Host species
Rabbit -
Specificity
The immunogen is homologous to human cytochrome P450 4A11 and 4A22. The specificity to these specific forms has not been confirmed experimentally. -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human Cytochrome P450 4A/CYP4A11 aa 50-150. The immunogen is homologous to human cytochrome P450 4A11 and 4A22.
-
Positive control
- Mouse liver, Rat liver, Human fetal kidney and fetal liver cell lysates. Human liver and kidney tissue.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8276 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab140635 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 59 kDa.
|
|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 59 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Catalyzes the omega- and (omega-1)-hydroxylation of various fatty acids such as laurate, myristate and palmitate. Has little activity toward prostaglandins A1 and E1. Oxidizes arachidonic acid to 20-hydroxyeicosatetraenoic acid (20-HETE). -
Tissue specificity
Kidney and liver. -
Sequence similarities
Belongs to the cytochrome P450 family. -
Cellular localization
Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
-
Database links
- Entrez Gene: 1579 Human
- Entrez Gene: 24306 Rat
- Entrez Gene: 298423 Rat
- Entrez Gene: 50549 Rat
- Omim: 601310 Human
- SwissProt: Q02928 Human
- SwissProt: P08516 Rat
- SwissProt: P20816 Rat
see all -
Alternative names
- 20-HETE synthase antibody
- 20-hydroxyeicosatetraenoic acid synthase antibody
- CP4AB_HUMAN antibody
see all
Images
-
All lanes : Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (ab140635) at 1/1000 dilution
Lane 1 : Mouse liver lysate
Lane 2 : Rat liver lysate
Lane 3 : Human fetal kidney lysate
Lane 4 : Human fetal liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 59 kDa -
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Cytochrome P450 4A/CYP4A11 with ab140635 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling Cytochrome P450 4A/CYP4A11 with ab140635 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin embedded Normal Human Brain tissue using ab140635 showing -ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin embedded Normal Human Breast tissue using ab140635 showing -ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin embedded Normal Human Colon tissue using ab140635 showing -ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin embedded Normal Human Liver tissue using ab140635 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin embedded Normal Human Tonsil tissue using ab140635 showing -ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (5)
ab140635 has been referenced in 5 publications.
- Kim S et al. Alteration of CYP4A11 expression in renal cell carcinoma: diagnostic and prognostic implications. J Cancer 11:1478-1485 (2020). PubMed: 32047554
- Zhou C et al. Gadofullerene inhibits the degradation of apolipoprotein B100 and boosts triglyceride transport for reversing hepatic steatosis. Sci Adv 6:N/A (2020). PubMed: 32917715
- Liu Y et al. Effects of daphnetin on lipid metabolism, insulin resistance and oxidative stress in OA-treated HepG2 cells. Mol Med Rep 19:4673-4684 (2019). PubMed: 30957185
- Morgan ET et al. Physiological Regulation of Drug Metabolism and Transport: Pregnancy, Microbiome, Inflammation, Infection, and Fasting. Drug Metab Dispos 46:503-513 (2018). PubMed: 29514828
- Nyagode BA et al. Selective effects of a therapeutic protein targeting tumor necrosis factor-alpha on cytochrome P450 regulation during infectious colitis: Implications for disease-dependent drug-drug interactions. Pharmacol Res Perspect 2:e00027 (2014). WB . PubMed: 24707356