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    cytochrome-p450-reductase-antibody-ab13513.pdf

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Anti-Cytochrome P450 Reductase antibody (ab13513)

  • Datasheet
  • SDS
Reviews (3)Q&A (4)References (21)

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Western blot - Anti-Cytochrome P450 Reductase antibody (ab13513)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 Reductase antibody (ab13513)

Key features and details

  • Rabbit polyclonal to Cytochrome P450 Reductase
  • Suitable for: WB, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG

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Recombinant Human Cytochrome P450 Reductase protein (ab151823)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Cytochrome P450 Reductase antibody
    See all Cytochrome P450 Reductase primary antibodies
  • Description

    Rabbit polyclonal to Cytochrome P450 Reductase
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Rat
    See all applications and species data
  • Immunogen

    Purified rat liver NADPH-Cytochrome P450 reductase.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cytochromes
    • Signal Transduction
    • Metabolism
    • Drug metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipases
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Drug metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Cytochromes
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Assay kits

    • Cytochrome P450 Reductase Activity Assay Kit (Colorimetric) (ab204704)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Cytochrome P450 Reductase protein (ab151823)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab13513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Rat
Application Abreviews Notes
WB (3)
1/1000. Detects a band of approximately 78 kDa.
ICC/IF
Use a concentration of 10 µg/ml.
Notes
WB
1/1000. Detects a band of approximately 78 kDa.
ICC/IF
Use a concentration of 10 µg/ml.

Target

  • Function

    This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.
  • Involvement in disease

    Defects in POR are the cause of adrenal hyperplasia variant type (AHV) [MIM:201750]; also known as Antley-Bixler syndrome-like phenotype with disordered steroidogenesis. AHV is a rare variant of congenital adrenal hyperplasia. It is an autosomal recessive disorder with apparent combined P450C17 and P450C21 deficiency. Affected girls are born with ambiguous genitalia, but their circulating androgens are low and virilization does not progress. Conversely, affected boys are sometimes born undermasculinized. Boys and girls can also present with bone malformations, in some cases resembling the pattern seen in patients with Antley-Bixler syndrome.
    Defects in POR are a cause of isolated disordered steroidogenesis (IDS) [MIM:201750].
  • Sequence similarities

    In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
  • Cellular localization

    Endoplasmic reticulum membrane. Anchored to the ER membrane by its N-terminal hydrophobic region.
  • Target information above from: UniProt accession P16435 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5447 Human
    • Entrez Gene: 29441 Rat
    • Omim: 124015 Human
    • SwissProt: P16435 Human
    • SwissProt: P00388 Rat
    • Unigene: 354056 Human
    • Unigene: 11359 Rat
    • Alternative names

      • CPR antibody
      • CYPOR antibody
      • Cytochrome p450 oxidoreductase antibody
      • DKFZp686G04235 antibody
      • FLJ26468 antibody
      • NADPH Cytochrome P450 Reductase antibody
      • NADPH dependent cytochrome P450 reductase antibody
      • NADPH--cytochrome P450 reductase antibody
      • NCPR_HUMAN antibody
      • P450 (cytochrome) oxidoreductase antibody
      • P450 Cytochrome Oxidoreductase antibody
      • P450R antibody
      • por antibody
      see all

    Images

    • Western blot - Anti-Cytochrome P450 Reductase antibody (ab13513)
      Western blot - Anti-Cytochrome P450 Reductase antibody (ab13513)
      Anti-Cytochrome P450 Reductase antibody (ab13513) at 1/2000 dilution + Cell lysates prepared from rat PC12 cells


      Western blot analysis of rat PC12 cell lysate, probed with ab13513.
    • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 Reductase antibody (ab13513)
      Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 Reductase antibody (ab13513)
      ICC/IF image of ab13513 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13513, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Protocols

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (21)

    Publishing research using ab13513? Please let us know so that we can cite the reference in this datasheet.

    ab13513 has been referenced in 21 publications.

    • Froeling FEM  et al. Bioactivation of Napabucasin Triggers Reactive Oxygen Species-Mediated Cancer Cell Death. Clin Cancer Res 25:7162-7174 (2019). PubMed: 31527169
    • Yao Y  et al. Effects and mechanism of amyloid ß1-42 on mitochondria in astrocytes. Mol Med Rep 17:6997-7004 (2018). PubMed: 29568933
    • Pankowicz FP  et al. Rapid Disruption of Genes Specifically in Livers of Mice Using Multiplex CRISPR/Cas9 Editing. Gastroenterology 155:1967-1970.e6 (2018). PubMed: 30170115
    • Yanatori I  et al. The iron chaperone poly(rC)-binding protein 2 forms a metabolon with the heme oxygenase 1/cytochrome P450 reductase complex for heme catabolism and iron transfer. J Biol Chem 292:13205-13229 (2017). PubMed: 28655775
    • Lo SN  et al. Berberine Activates Aryl Hydrocarbon Receptor but Suppresses CYP1A1 Induction through miR-21-3p Stimulation in MCF-7 Breast Cancer Cells. Molecules 22:N/A (2017). PubMed: 29143794
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-7 of 7 Abreviews or Q&A

    Western blot abreview for Anti-Cytochrome P450 Reductase antibody

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (transfection of GFP tagged Human CYPred)
    Loading amount
    40000 cells
    Specification
    transfection of GFP tagged Human CYPred
    Treatment
    1x laemmli buffer, 10 min boiling,
    Gel Running Conditions
    Non-reduced Denaturing (4-20% TGX biorad)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Ms. Anette Orjuela

    Verified customer

    Submitted Feb 23 2012

    Question

    We purchased two antibodies mouse monoclonal to Calreticulin(ab22683) and Rabbit polyclonal to Cytochrome P450 reductase(AB13513) in November. I used them to stain mouse hippocampal sections. The antibody for Cytochrome P450 works very well. The antibody for Calreticulin (ab22683) produces some non-specific staining. When I received the package, instead of just 2 datasheets, there were datasheets for 3 antibodies. One for ab22683 (calreticulin), one for ab13513 (cytochrome P450) and the third one for ab61392 (mouse monoclonal to 3-nitrotyrosine). I have a concern if the antibody for 3-nitrotyrosine was shipped under the wrong label as calreticulin. I am attaching a ppt. slide with the images I got using calreticulin antibody. Can you please check it and let me know if there might be some labeling error with the antibody (or) provide any suggestion. Some details about my Immunocytochemistry protocol: Fixative – 4% Paraformaldehyde + 0.1% Glutaraladehyde Primary Antibody – ab 22683 (Mouse monoclonal to calreticulin) - Incubated for 24hrs at 4C Secondary antibody – Donkey anti-mouse Alexa FluoR 555 (from Invitrogen) – Incubated for 2hrs at room temperature Blocking buffer – 0.3% Bovine Serum Albumin in PBS

    Read More

    Abcam community

    Verified customer

    Asked on Jan 05 2012

    Answer

    Thank you for bringing this to our attention and for sending your protocol and IHC images. I am sorry to see that the antibody is giving you trouble. It is unlikely, but possible, that the wrong antibody was delivered to you. We have not received other complaints about the antibody so I do not think there was a labeling mix-up. The antibody may itself be defective, at least with these mouse samples. I am wondering if the glutaraldehyde that you include in the fixative might be creating some of the background but your negative control appears relatively clean. Some people will add glycine at 0.3M to the blocking solution to quench unreacted aldehydes. In any case, there is no staining of the ER, as far as I can tell. Can you tell me how long your samples were fixed, how much antibody you applied (concentration or dilution), and if you are confident that the secondary antibody is effective? For a replacement or refund or credit, I will also need the order reference number, either Abcam's or the PO number. I look forward to your reply and to resolving this issue.

    Read More

    Abcam Scientific Support

    Answered on Jan 05 2012

    Question

    Is this Cytochrome P450-reductase antibody affinity purified?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 15 2011

    Answer

    Thank you for contacting us with your question. Ab13513 is provided as whole antiserum and is not a purified antibody. Some of our other cytochrome P450 reductase antibodies are affinity purified (such as ab39995) and we also have purification kits that can be used to isolate the IgG molecules from the antiserum- https://www.abcam.com/Antibody-Serum-Purification-kit-1-purification-ab109208.html I hope this information will be useful in determining whether this antibody is appropriate for your studies. If you have any further questions or need any other information please let me know.

    Read More

    Abcam Scientific Support

    Answered on Oct 15 2011

    Western blot abreview for Anti-Cytochrome P450 Reductase antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Liver whole homogenate)
    Loading amount
    10 µg
    Specification
    Liver whole homogenate
    Gel Running Conditions
    Reduced Denaturing (12 %)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 21 2008

    Western blot abreview for Anti-Cytochrome P450 Reductase antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - other (BS2B Human Lung Cells)
    Loading amount
    30 µg
    Specification
    BS2B Human Lung Cells
    Blocking step
    BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Mr. Adam Szadkowski

    Verified customer

    Submitted Jun 15 2007

    Question

    What do you recommend as positive and negative controls for these antibodies?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 27 2006

    Answer

    Thank you for contacting us. For ab23677, LTA4 hydrolase, I suggest either a neutrophil or monocyte lysate. We carry THP1 lysate, ab7913, which is a human monocytic leukemia. For a negative control, LTA4 was detected in epidermal keratinocytes and endothelial cells but not whole skin in the following paper: Leukotriene A4 hydrolase in human skin. J Invest Dermatol. 1994 Feb;102(2):253-7. PMID: 8106755 Our lysate ab30166 may be suitable as a negative control but I cannot guarantee you will not see some reactivity. For the cytochrome P450 enzymes, I suggest liver lysates. A positive control for ab4236 is human liver: ab29889. For ab13513 and ab3573, rat liver lysate ab29413 will be appropriate. I am reluctant to recommend a negative control since information regarding absence of expression is difficult to come by but since CP450 is associated with the ER, cytoplasmic fractions of liver lysates (which do not contain membrane) may be useful. For human liver, this would be ab29875, and for rat liver ab29409. You should also have a look at the references listed on the datasheets to see how other researchers have used the antibodies, though the references for the LTA4 are general and not specific to the antibody. I hope this helps. Please do not hesitate to contact us if you have any other questions.

    Read More

    Abcam Scientific Support

    Answered on Nov 27 2006

    Question

    Do you have an image of this antibody in Western blot and immunocytochemistry?

    Read More

    Abcam community

    Verified customer

    Asked on May 09 2006

    Answer

    Thank you for your enquiry. I was able to obtain a Western blot image, which I have added to our datasheet. The antibody was used in WB in this paper, http://cancerres.aacrjournals.org/cgi/content/full/60/13/3550 I hope this information helps. Please do not hesitate to contact us if you need anything further.

    Read More

    Abcam Scientific Support

    Answered on May 11 2006

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