Anti-Cytochrome P450 Reductase antibody (ab13513)
- Datasheet
- Specific References (15)
- Protocols
Overview
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Product nameAnti-Cytochrome P450 Reductase antibody
See all Cytochrome P450 Reductase primary antibodies -
DescriptionRabbit polyclonal to Cytochrome P450 Reductase
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Host speciesRabbit
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Tested applicationsSuitable for: ICC, IP, WB, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Mouse, Rat, Sheep, Rabbit, Guinea pig, Hamster, Cow, Dog, Human, Pig, Monkey
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Immunogen
Purified rat liver NADPH-Cytochrome P450 reductase.
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Positive control
- Rat Liver NADPH Cytochrome P450 Reductase Protein.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, PBS, pH 7.2 -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Assay kits
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab13513 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC | 1/500 - 1/1000. | |
| IP | Use a concentration of 5 µg/ml. | |
| WB | 1/1000. Detects a band of approximately 78 kDa. | |
| IHC-P | 1/500. | |
| ICC/IF | Use a concentration of 10 µg/ml. |
Target
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FunctionThis enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.
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Involvement in diseaseDefects in POR are the cause of adrenal hyperplasia variant type (AHV) [MIM:201750]; also known as Antley-Bixler syndrome-like phenotype with disordered steroidogenesis. AHV is a rare variant of congenital adrenal hyperplasia. It is an autosomal recessive disorder with apparent combined P450C17 and P450C21 deficiency. Affected girls are born with ambiguous genitalia, but their circulating androgens are low and virilization does not progress. Conversely, affected boys are sometimes born undermasculinized. Boys and girls can also present with bone malformations, in some cases resembling the pattern seen in patients with Antley-Bixler syndrome.
Defects in POR are a cause of isolated disordered steroidogenesis (IDS) [MIM:201750]. -
Sequence similaritiesIn the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain. -
Cellular localizationEndoplasmic reticulum membrane. Anchored to the ER membrane by its N-terminal hydrophobic region.
- Information by UniProt
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Database links
- Entrez Gene: 532512 Cow
- Entrez Gene: 489816 Dog
- Entrez Gene: 100135602 Guinea pig
- Entrez Gene: 5447 Human
- Entrez Gene: 18984 Mouse
- Entrez Gene: 100170114 Pig
- Entrez Gene: 100301554 Rabbit
- Entrez Gene: 29441 Rat
see all -
Alternative names
- CPR antibody
- CYPOR antibody
- Cytochrome p450 oxidoreductase antibody
see all
Images
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Western blot - Anti-Cytochrome P450 Reductase antibody (ab13513)Anti-Cytochrome P450 Reductase antibody (ab13513) at 1/2000 dilution + Cell lysates prepared from rat PC12 cells
Western blot analysis of rat PC12 cell lysate, probed with ab13513. -
Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 Reductase antibody (ab13513)ICC/IF image of ab13513 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13513, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunoprecipitation - Anti-Cytochrome P450 Reductase antibody (ab13513)Cytochrome P450 Reductase was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Cytochrome P450 Reductase and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab13513.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 77kDa: Cytochrome P450 Reductase
Protocols
References
This product has been referenced in:
- Yao Y et al. Effects and mechanism of amyloid ß1-42 on mitochondria in astrocytes. Mol Med Rep 17:6997-7004 (2018). Read more (PubMed: 29568933) »
- Barzi M et al. A novel humanized mouse lacking murine P450 oxidoreductase for studying human drug metabolism. Nat Commun 8:39 (2017). WB, IHC-Fr . Read more (PubMed: 28659616) »
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