Overview

  • Product name

    Anti-Cytochrome P450 Reductase antibody
    See all Cytochrome P450 Reductase primary antibodies
  • Description

    Rabbit polyclonal to Cytochrome P450 Reductase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, IP, WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Sheep, Rabbit, Guinea pig, Hamster, Cow, Dog, Human, Pig, Monkey
  • Immunogen

    Purified rat liver NADPH-Cytochrome P450 reductase.

  • Positive control

    • Rat Liver NADPH Cytochrome P450 Reductase Protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab13513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/500 - 1/1000.
IP Use a concentration of 5 µg/ml.
WB 1/1000. Detects a band of approximately 78 kDa.
IHC-P 1/500.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function

    This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.
  • Involvement in disease

    Defects in POR are the cause of adrenal hyperplasia variant type (AHV) [MIM:201750]; also known as Antley-Bixler syndrome-like phenotype with disordered steroidogenesis. AHV is a rare variant of congenital adrenal hyperplasia. It is an autosomal recessive disorder with apparent combined P450C17 and P450C21 deficiency. Affected girls are born with ambiguous genitalia, but their circulating androgens are low and virilization does not progress. Conversely, affected boys are sometimes born undermasculinized. Boys and girls can also present with bone malformations, in some cases resembling the pattern seen in patients with Antley-Bixler syndrome.
    Defects in POR are a cause of isolated disordered steroidogenesis (IDS) [MIM:201750].
  • Sequence similarities

    In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
  • Cellular localization

    Endoplasmic reticulum membrane. Anchored to the ER membrane by its N-terminal hydrophobic region.
  • Information by UniProt
  • Database links

  • Alternative names

    • CPR antibody
    • CYPOR antibody
    • Cytochrome p450 oxidoreductase antibody
    • DKFZp686G04235 antibody
    • FLJ26468 antibody
    • NADPH Cytochrome P450 Reductase antibody
    • NADPH dependent cytochrome P450 reductase antibody
    • NADPH--cytochrome P450 reductase antibody
    • NCPR_HUMAN antibody
    • P450 (cytochrome) oxidoreductase antibody
    • P450 Cytochrome Oxidoreductase antibody
    • P450R antibody
    • por antibody
    see all

Images

  • Anti-Cytochrome P450 Reductase antibody (ab13513) at 1/2000 dilution + Cell lysates prepared from rat PC12 cells


    Western blot analysis of rat PC12 cell lysate, probed with ab13513.
  • ICC/IF image of ab13513 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13513, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Cytochrome P450 Reductase was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Cytochrome P450 Reductase and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab13513.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 77kDa: Cytochrome P450 Reductase

References

This product has been referenced in:

  • Yao Y  et al. Effects and mechanism of amyloid ß1-42 on mitochondria in astrocytes. Mol Med Rep 17:6997-7004 (2018). Read more (PubMed: 29568933) »
  • Pankowicz FP  et al. Rapid Disruption of Genes Specifically in Livers of Mice Using Multiplex CRISPR/Cas9 Editing. Gastroenterology 155:1967-1970.e6 (2018). Read more (PubMed: 30170115) »
See all 19 Publications for this product

Customer reviews and Q&As

Filter by Application

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1-3 of 3 Abreviews

Application
Western blot
Sample
Human Cell lysate - whole cell (transfection of GFP tagged Human CYPred)
Loading amount
40000 cells
Specification
transfection of GFP tagged Human CYPred
Treatment
1x laemmli buffer, 10 min boiling,
Gel Running Conditions
Non-reduced Denaturing (4-20% TGX biorad)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Ms. Anette Orjuela

Verified customer

Submitted Feb 23 2012

Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver whole homogenate)
Loading amount
10 µg
Specification
Liver whole homogenate
Gel Running Conditions
Reduced Denaturing (12 %)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Apr 21 2008

Application
Western blot
Sample
Human Cell lysate - other (BS2B Human Lung Cells)
Loading amount
30 µg
Specification
BS2B Human Lung Cells
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5%

Mr. Adam Szadkowski

Verified customer

Submitted Jun 15 2007

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