Overview

  • Product name

    Anti-Cytochrome P450 Reductase antibody
    See all Cytochrome P450 Reductase primary antibodies
  • Description

    Rabbit polyclonal to Cytochrome P450 Reductase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Cytochrome P450 Reductase (N terminal). The exact sequence is proprietary.
    Database link: P16435

  • Positive control

    • WB: HeLa cell extract; Mouse lung lysate. ICC/IF: HeLa cells. IHC-P: Human ovarian carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab227071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 76 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.
  • Involvement in disease

    Defects in POR are the cause of adrenal hyperplasia variant type (AHV) [MIM:201750]; also known as Antley-Bixler syndrome-like phenotype with disordered steroidogenesis. AHV is a rare variant of congenital adrenal hyperplasia. It is an autosomal recessive disorder with apparent combined P450C17 and P450C21 deficiency. Affected girls are born with ambiguous genitalia, but their circulating androgens are low and virilization does not progress. Conversely, affected boys are sometimes born undermasculinized. Boys and girls can also present with bone malformations, in some cases resembling the pattern seen in patients with Antley-Bixler syndrome.
    Defects in POR are a cause of isolated disordered steroidogenesis (IDS) [MIM:201750].
  • Sequence similarities

    In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
  • Cellular localization

    Endoplasmic reticulum membrane. Anchored to the ER membrane by its N-terminal hydrophobic region.
  • Information by UniProt
  • Database links

  • Alternative names

    • CPR antibody
    • CYPOR antibody
    • Cytochrome p450 oxidoreductase antibody
    • DKFZp686G04235 antibody
    • FLJ26468 antibody
    • NADPH Cytochrome P450 Reductase antibody
    • NADPH dependent cytochrome P450 reductase antibody
    • NADPH--cytochrome P450 reductase antibody
    • NCPR_HUMAN antibody
    • P450 (cytochrome) oxidoreductase antibody
    • P450 Cytochrome Oxidoreductase antibody
    • P450R antibody
    • por antibody
    see all

Images

  • All lanes : Anti-Cytochrome P450 Reductase antibody (ab227071) at 1/500 dilution

    Lane 1 : Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
    Lane 2 : Cytochrome P450 Reductase knock out HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 76 kDa



    7.5% SDS-PAGE gel.

  • Anti-Cytochrome P450 Reductase antibody (ab227071) at 1/500 dilution + Mouse lung lysate at 50 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 76 kDa



    7.5% SDS-PAGE gel.

  • Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Cytochrome P450 Reductase (green) using ab227071 at 1/200 dilution in ICC/IF.

    Lower panel: Costained with Hoechst 33342.

  • Paraffin-embedded human ovarian carcinoma tissue stained for Cytochrome P450 Reductase using ab227071 at 1/500 dilution in immunohistochemical analysis.

References

ab227071 has not yet been referenced specifically in any publications.

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