Key features and details
- Mouse polyclonal to Cytochrome P450 Reductase
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Cytochrome P450 Reductase antibody
See all Cytochrome P450 Reductase primary antibodies
DescriptionMouse polyclonal to Cytochrome P450 Reductase
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Full length Human protein (AAH34277.1)
- WB: liver lysate. ICC/IF: HeLa cells.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.4
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein A purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
Our Abpromise guarantee covers the use of ab89622 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 77 kDa.|
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionThis enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.
Involvement in diseaseDefects in POR are the cause of adrenal hyperplasia variant type (AHV) [MIM:201750]; also known as Antley-Bixler syndrome-like phenotype with disordered steroidogenesis. AHV is a rare variant of congenital adrenal hyperplasia. It is an autosomal recessive disorder with apparent combined P450C17 and P450C21 deficiency. Affected girls are born with ambiguous genitalia, but their circulating androgens are low and virilization does not progress. Conversely, affected boys are sometimes born undermasculinized. Boys and girls can also present with bone malformations, in some cases resembling the pattern seen in patients with Antley-Bixler syndrome.
Defects in POR are a cause of isolated disordered steroidogenesis (IDS) [MIM:201750].
Sequence similaritiesIn the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain.
Cellular localizationEndoplasmic reticulum membrane. Anchored to the ER membrane by its N-terminal hydrophobic region.
- Information by UniProt
- CPR antibody
- CYPOR antibody
- Cytochrome p450 oxidoreductase antibody
Anti-Cytochrome P450 Reductase antibody (ab89622) at 1 µg/ml + Human liver lysate at 50 µg
Predicted band size: 77 kDa
All lanes : Anti-Cytochrome P450 Reductase antibody (ab89622) at 1 µg/ml
Lane 1 : Cytochrome P450 Reductase transfected 293T cell lysate
Lane 2 : Untransfected lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 77 kDa
Immunofluorescence analysis of Cytochrome P450 Reductase expression in PFA-fixed HeLa cells using ab89622 at 10 µg/ml.
ab89622 has not yet been referenced specifically in any publications.