Overview

  • Product name

    Anti-Cytochrome P450 Reductase antibody
    See all Cytochrome P450 Reductase primary antibodies
  • Description

    Mouse polyclonal to Cytochrome P450 Reductase
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length Human protein (AAH34277.1)

  • Positive control

    • WB: liver lysate. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab89622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 77 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function

    This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.
  • Involvement in disease

    Defects in POR are the cause of adrenal hyperplasia variant type (AHV) [MIM:201750]; also known as Antley-Bixler syndrome-like phenotype with disordered steroidogenesis. AHV is a rare variant of congenital adrenal hyperplasia. It is an autosomal recessive disorder with apparent combined P450C17 and P450C21 deficiency. Affected girls are born with ambiguous genitalia, but their circulating androgens are low and virilization does not progress. Conversely, affected boys are sometimes born undermasculinized. Boys and girls can also present with bone malformations, in some cases resembling the pattern seen in patients with Antley-Bixler syndrome.
    Defects in POR are a cause of isolated disordered steroidogenesis (IDS) [MIM:201750].
  • Sequence similarities

    In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
  • Cellular localization

    Endoplasmic reticulum membrane. Anchored to the ER membrane by its N-terminal hydrophobic region.
  • Information by UniProt
  • Database links

  • Alternative names

    • CPR antibody
    • CYPOR antibody
    • Cytochrome p450 oxidoreductase antibody
    • DKFZp686G04235 antibody
    • FLJ26468 antibody
    • NADPH Cytochrome P450 Reductase antibody
    • NADPH dependent cytochrome P450 reductase antibody
    • NADPH--cytochrome P450 reductase antibody
    • NCPR_HUMAN antibody
    • P450 (cytochrome) oxidoreductase antibody
    • P450 Cytochrome Oxidoreductase antibody
    • P450R antibody
    • por antibody
    see all

Images

  • Anti-Cytochrome P450 Reductase antibody (ab89622) at 1 µg/ml + Human liver lysate at 50 µg

    Predicted band size: 77 kDa

  • All lanes : Anti-Cytochrome P450 Reductase antibody (ab89622) at 1 µg/ml

    Lane 1 : Cytochrome P450 Reductase transfected 293T cell lysate
    Lane 2 : Untransfected lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 77 kDa

  • Immunofluorescence analysis of Cytochrome P450 Reductase expression in PFA-fixed HeLa cells using ab89622 at 10 µg/ml.

References

ab89622 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (HEK283T)
Loading amount
200000 cells
Specification
HEK283T
Gel Running Conditions
Non-reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 20 2012

Answer

DISCOUNT CODE: **********
Expiration date: ************
I am very pleased to hear you would like to accept our offer and test ab89622 in mouse tissue. You are correct that you have to purchase the antibody ab89622 first, and then after submitting the Abreview, the discount code will become active and you can use it to get a second antibody free of charge.
The above code will give you 1 free PRIMARY ANTIBODYbefore the expiration date. To redeem this offer, please submit an Abreview for mouse tissue and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you very much for your interest in ab89622.
We do not provide sample sizes of our antibodies but as we have not tested ab89622 in mouse tissue (although there is a 92% level of homology between human an mouse, so there is a very good chance that it will work), I can offer a discount off a future purchase if you buy ab89622 now, test it inmouse tissue and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY.
If you are interested in this offer, please follow these steps:
1. Reply to this e-mail to let me know that you would like to proceed and test ab89622 in mouse tissue. I will then send a discount code. This code must be issued before purchasing ab89622 so please wait for my reply before ordering.
2. Purchase ab89622 either by phone, fax, or online (www.abcam.com).
3. Test it in mouse tissue.
4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.
5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.
We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab89622 turns out to be unsuitable for mouse tissue, you will still receive the discount on your next purchase after your Abreview has been submitted.
Please let me know if you have any questions about this offer and I would be happy to help you further.
The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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