Overview

  • Product name

    Anti-Cytohesin 1 antibody [EPR10050]
    See all Cytohesin 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR10050] to Cytohesin 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IP, Flow Cytmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Cytohesin 1 (Uniprot: Q15438)

  • Positive control

    • Raji, Jurkat and HuT-78 cell lysates; Human colon tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR10050
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 46 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function

      Promotes guanine-nucleotide exchange on ARF1 and ARF5. Promotes the activation of ARF through replacement of GDP with GTP.
    • Tissue specificity

      Ubiquitous.
    • Sequence similarities

      Contains 1 PH domain.
      Contains 1 SEC7 domain.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • B2 1 antibody
      • CLM1 antibody
      • CTH 1 antibody
      • CYH1_HUMAN antibody
      • CYTH1 antibody
      • CYTIP antibody
      • Cytoadhesin 1 antibody
      • CYTOHESIN 1 antibody
      • Cytohesin-1 antibody
      • D17S811E antibody
      • FLJ34050 antibody
      • FLJ41900 antibody
      • Homolog of secretory protein SEC7 antibody
      • KIAA4240 antibody
      • mKIAA4240 antibody
      • mSec7 1 antibody
      • OTTHUMP00000196778 antibody
      • OTTHUMP00000196779 antibody
      • PH antibody
      • PH, SEC7 and coiled-coil domain containing protein 1 antibody
      • Pleckstrin homology Sec7 and coiled/coil domains 1 antibody
      • Pleckstrin homology Sec7 and coiled/coil domains protein 1 antibody
      • Pleckstrin homology, Sec7 and coiled coil domains 1 antibody
      • PSCD1 antibody
      • SEC7 and coiled-coil domain-containing protein 1 antibody
      • Sec7 antibody
      • SEC7 homolog A antibody
      • SEC7 homolog B2 1 antibody
      • SEC7 homolog B2-1 antibody
      • SEC7, yeast, homolog of antibody
      • Sec7a antibody
      see all

    Images

    • All lanes : Anti-Cytohesin 1 antibody [EPR10050] (ab154847) at 1/1000 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HuT-78 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 46 kDa

    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue, labeling Cytohesin 1 using ab154847 at a 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Chen X  et al. Comprehensive Gene Expression Analysis in NMIBC Using RNA-seq Reveals New Therapy Strategies. Front Oncol 9:523 (2019). Read more (PubMed: 31293967) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab154847.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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