Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10050] to Cytohesin 1
- Suitable for: WB, IHC-P, IP, Flow Cyt
- Reacts with: Human
Product nameAnti-Cytohesin 1 antibody [EPR10050]
See all Cytohesin 1 primary antibodies
DescriptionRabbit monoclonal [EPR10050] to Cytohesin 1
Tested applicationsSuitable for: WB, IHC-P, IP, Flow Cytmore details
Unsuitable for: ICC/IF
Species reactivityReacts with: Human
Synthetic peptide corresponding to residues in Human Cytohesin 1 (Uniprot: Q15438)
- Raji, Jurkat and HuT-78 cell lysates; Human colon tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab154847 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 46 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionPromotes guanine-nucleotide exchange on ARF1 and ARF5. Promotes the activation of ARF through replacement of GDP with GTP.
Sequence similaritiesContains 1 PH domain.
Contains 1 SEC7 domain.
Cellular localizationCell membrane.
- Information by UniProt
- B2 1 antibody
- CLM1 antibody
- CTH 1 antibody
All lanes : Anti-Cytohesin 1 antibody [EPR10050] (ab154847) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HuT-78 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 46 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue, labeling Cytohesin 1 using ab154847 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab154847 has been referenced in 1 publication.
- Chen X et al. Comprehensive Gene Expression Analysis in NMIBC Using RNA-seq Reveals New Therapy Strategies. Front Oncol 9:523 (2019). PubMed: 31293967