Recombinant Anti-Cytohesin 1 antibody [EPR10051] (ab151732)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10051] to Cytohesin 1
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Cytohesin 1 antibody [EPR10051]
See all Cytohesin 1 primary antibodies -
Description
Rabbit monoclonal [EPR10051] to Cytohesin 1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Cytohesin 1 aa 300-400. The exact sequence is proprietary.
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Positive control
- Jurkat, Raji, K562 and HUT78 cell lysates; HUT78 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10051 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151732 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 46 kDa.
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Notes |
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 46 kDa. |
Target
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Function
Promotes guanine-nucleotide exchange on ARF1 and ARF5. Promotes the activation of ARF through replacement of GDP with GTP. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Contains 1 PH domain.
Contains 1 SEC7 domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 9267 Human
- Entrez Gene: 19157 Mouse
- Entrez Gene: 116691 Rat
- Omim: 182115 Human
- SwissProt: Q15438 Human
- SwissProt: Q9QX11 Mouse
- SwissProt: P97694 Rat
- Unigene: 191215 Human
see all -
Alternative names
- B2 1 antibody
- CLM1 antibody
- CTH 1 antibody
see all
Images
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All lanes : Anti-Cytohesin 1 antibody [EPR10051] (ab151732) at 1/1000 dilution
Lane 1 : Jurkat cell lysates
Lane 2 : Raji cell lysates
Lane 3 : K562 cell lysates
Lane 4 : HUT78 cell lysates
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 46 kDa -
Intracellular flow cytometric analysis of permeabilized HUT78 cells labeling Cytohesin 1 using ab151732 at a 1/100 dilution (red) or a rabbit IgG control (negative) (green).
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab151732 has not yet been referenced specifically in any publications.