Recombinant Anti-Cytohesin 1 antibody [EPR10051] (ab151732)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10051] to Cytohesin 1
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Cytohesin 1 antibody [EPR10051]
See all Cytohesin 1 primary antibodies -
Description
Rabbit monoclonal [EPR10051] to Cytohesin 1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Cytohesin 1 aa 300-400. The exact sequence is proprietary.
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Positive control
- Jurkat, Raji, K562 and HUT78 cell lysates; HUT78 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10051 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab151732 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt | 1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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WB | 1/1000 - 1/10000. Predicted molecular weight: 46 kDa. |
Target
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Function
Promotes guanine-nucleotide exchange on ARF1 and ARF5. Promotes the activation of ARF through replacement of GDP with GTP. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Contains 1 PH domain.
Contains 1 SEC7 domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 9267 Human
- Entrez Gene: 19157 Mouse
- Entrez Gene: 116691 Rat
- Omim: 182115 Human
- SwissProt: Q15438 Human
- SwissProt: Q9QX11 Mouse
- SwissProt: P97694 Rat
- Unigene: 191215 Human
see all -
Alternative names
- B2 1 antibody
- CLM1 antibody
- CTH 1 antibody
see all
Images
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All lanes : Anti-Cytohesin 1 antibody [EPR10051] (ab151732) at 1/1000 dilution
Lane 1 : Jurkat cell lysates
Lane 2 : Raji cell lysates
Lane 3 : K562 cell lysates
Lane 4 : HUT78 cell lysates
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 46 kDa -
Flow cytometric analysis of permeabilized HUT78 cells labeling Cytohesin 1 using ab151732 at a 1/100 dilution (red) or a rabbit IgG control (negative) (green).
Datasheets and documents
References (0)
ab151732 has not yet been referenced specifically in any publications.