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Signal Transduction Protein Trafficking Vesicle Transport Regulation
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RecombinantRabMAb

Recombinant Anti-Cytohesin 1 antibody [EPR10051] (ab151732)

  • Datasheet
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Western blot - Anti-Cytohesin 1 antibody [EPR10051] (ab151732)
  • Flow Cytometry - Anti-Cytohesin 1 antibody [EPR10051] (ab151732)
  • Anti-Cytohesin 1 antibody [EPR10051] (ab151732)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10051] to Cytohesin 1
  • Suitable for: Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Protein
Product image
Recombinant Human Cytohesin 1 protein (ab202206)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Cytohesin 1 antibody [EPR10051]
    See all Cytohesin 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR10051] to Cytohesin 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Cytohesin 1 aa 300-400. The exact sequence is proprietary.

  • Positive control

    • Jurkat, Raji, K562 and HUT78 cell lysates; HUT78 cells
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR10051
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation

Associated products

  • Alternative Versions

    • Anti-Cytohesin 1 antibody [EPR10051] - BSA and Azide free (ab249049)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Raji whole cell lysate (ab30124)
    • Raji membrane extract lysate (ab30126)
    • Jurkat whole cell lysate (ab7899)
    • K562 whole cell lysate (ab7911)
  • Recombinant Protein

    • Recombinant Human Cytohesin 1 protein (ab202206)

Applications

Our Abpromise guarantee covers the use of ab151732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/1000 - 1/10000. Predicted molecular weight: 46 kDa.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Promotes guanine-nucleotide exchange on ARF1 and ARF5. Promotes the activation of ARF through replacement of GDP with GTP.
    • Tissue specificity

      Ubiquitous.
    • Sequence similarities

      Contains 1 PH domain.
      Contains 1 SEC7 domain.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession Q15438 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 9267 Human
      • Entrez Gene: 19157 Mouse
      • Entrez Gene: 116691 Rat
      • Omim: 182115 Human
      • SwissProt: Q15438 Human
      • SwissProt: Q9QX11 Mouse
      • SwissProt: P97694 Rat
      • Unigene: 191215 Human
      • Unigene: 86413 Mouse
      • Unigene: 10672 Rat
      see all
    • Alternative names

      • B2 1 antibody
      • CLM1 antibody
      • CTH 1 antibody
      • CYH1_HUMAN antibody
      • CYTH1 antibody
      • CYTIP antibody
      • Cytoadhesin 1 antibody
      • CYTOHESIN 1 antibody
      • Cytohesin-1 antibody
      • D17S811E antibody
      • FLJ34050 antibody
      • FLJ41900 antibody
      • Homolog of secretory protein SEC7 antibody
      • KIAA4240 antibody
      • mKIAA4240 antibody
      • mSec7 1 antibody
      • OTTHUMP00000196778 antibody
      • OTTHUMP00000196779 antibody
      • PH antibody
      • PH, SEC7 and coiled-coil domain containing protein 1 antibody
      • Pleckstrin homology Sec7 and coiled/coil domains 1 antibody
      • Pleckstrin homology Sec7 and coiled/coil domains protein 1 antibody
      • Pleckstrin homology, Sec7 and coiled coil domains 1 antibody
      • PSCD1 antibody
      • SEC7 and coiled-coil domain-containing protein 1 antibody
      • Sec7 antibody
      • SEC7 homolog A antibody
      • SEC7 homolog B2 1 antibody
      • SEC7 homolog B2-1 antibody
      • SEC7, yeast, homolog of antibody
      • Sec7a antibody
      see all

    Images

    • Western blot - Anti-Cytohesin 1 antibody [EPR10051] (ab151732)
      Western blot - Anti-Cytohesin 1 antibody [EPR10051] (ab151732)
      All lanes : Anti-Cytohesin 1 antibody [EPR10051] (ab151732) at 1/1000 dilution

      Lane 1 : Jurkat cell lysates
      Lane 2 : Raji cell lysates
      Lane 3 : K562 cell lysates
      Lane 4 : HUT78 cell lysates

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 46 kDa

    • Flow Cytometry - Anti-Cytohesin 1 antibody [EPR10051] (ab151732)
      Flow Cytometry - Anti-Cytohesin 1 antibody [EPR10051] (ab151732)
      Flow cytometric analysis of permeabilized HUT78 cells labeling Cytohesin 1 using ab151732 at a 1/100 dilution (red) or a rabbit IgG control (negative) (green).
    • Anti-Cytohesin 1 antibody [EPR10051] (ab151732)
      Anti-Cytohesin 1 antibody [EPR10051] (ab151732)

    Protocols

    • Western blot protocols
    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab151732? Please let us know so that we can cite the reference in this datasheet.

    ab151732 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    There are currently no Customer reviews or Questions for ab151732.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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