Product nameCytohesin 1 overexpression 293T lysate (whole cell)
DescriptionCytohesin 1 overexpression lysate
ab94252 is a 293T cell transfected lysate in which Human Cytohesin 1 has been transiently over-expressed using a pCMV-Cytohesin 1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 6.80
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol
Concentration information loading...
BackgroundFunction: Promotes guanine-nucleotide exchange on ARF1 and ARF5. Promotes the activation of ARF through replacement of GDP with GTP. Tissue specificity: Ubiquitous. Similarity: Contains 1 PH domain. Contains 1 SEC7 domain.
Our Abpromise guarantee covers the use of ab94252 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94252 at 15μg/lane on an SDS-PAGE gel
All lanes : Anti-Cytohesin 1 antibody (ab56511) at 1/500 dilution
Lane 1 :
Cytohesin 1 overexpression 293T lysate (whole cell) (ab94252)
Lane 2 : 293T Non Transfected Lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94252 has not yet been referenced specifically in any publications.