Key features and details
- Rabbit polyclonal to Cytohesin 2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Cytohesin 2 antibody
See all Cytohesin 2 primary antibodies
DescriptionRabbit polyclonal to Cytohesin 2
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human Cytohesin 2 aa 150-350.
Database link: Q99418
- WB: U-251 MG, HepG2, MCF7 and A549 whole cell lysates. IHC-P: Human ovarian cancer and colon cancer tissues.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Detects a band of approximately 35,47 kDa (predicted molecular weight: 47 kDa).|
FunctionActs as a guanine-nucleotide exchange factor (GEF). Promotes guanine-nucleotide exchange on ARF1, ARF3 and ARF6. Promotes the activation of ARF factors through replacement of GDP with GTP. The cell membrane form, in association with ARL4 proteins, recruits ARF6 to the plasma membrane.
Sequence similaritiesContains 1 PH domain.
Contains 1 SEC7 domain.
DomainThe PH domain is necessary and sufficient for plasma membrane relocalization.
Cellular localizationCell membrane. Cytoplasm. Both isoform 1 and isoform 2 are recruited to the cell membrane through its association with ARL4A, ARL4C and ARL4D. Requires also interaction with phosphoinositides for targeting to plasma membrane.
- Information by UniProt
- ARF exchange factor antibody
- ARF nucleotide binding site opener antibody
- ARF nucleotide-binding site opener antibody
All lanes : Anti-Cytohesin 2 antibody (ab229994) at 1/1000 dilution
Lane 1 : U-251 MG (human brain glioma cell line) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : A549 (human lung carcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 47 kDa
Paraffin-embedded human ovarian cancer tissue stained for Cytohesin 2 using ab229994 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human colon cancer tissue stained for Cytohesin 2 using ab229994 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229994 has not yet been referenced specifically in any publications.