Anti-Cytohesin 2 antibody (ab229994)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Cytohesin 2 antibody
See all Cytohesin 2 primary antibodies -
Description
Rabbit polyclonal to Cytohesin 2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Cytohesin 2 aa 150-350.
Database link: Q99418 -
Positive control
- WB: U-251 MG, HepG2, MCF7 and A549 whole cell lysates. IHC-P: Human ovarian cancer and colon cancer tissues.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab229994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/20 - 1/200. | |
| WB | 1/1000 - 1/5000. Detects a band of approximately 35,47 kDa (predicted molecular weight: 47 kDa). |
Target
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Function
Acts as a guanine-nucleotide exchange factor (GEF). Promotes guanine-nucleotide exchange on ARF1, ARF3 and ARF6. Promotes the activation of ARF factors through replacement of GDP with GTP. The cell membrane form, in association with ARL4 proteins, recruits ARF6 to the plasma membrane. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Contains 1 PH domain.
Contains 1 SEC7 domain. -
Domain
The PH domain is necessary and sufficient for plasma membrane relocalization. -
Cellular localization
Cell membrane. Cytoplasm. Both isoform 1 and isoform 2 are recruited to the cell membrane through its association with ARL4A, ARL4C and ARL4D. Requires also interaction with phosphoinositides for targeting to plasma membrane. - Information by UniProt
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Database links
- Entrez Gene: 9266 Human
- Omim: 602488 Human
- SwissProt: Q99418 Human
- Unigene: 144011 Human
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Alternative names
- ARF exchange factor antibody
- ARF nucleotide binding site opener antibody
- ARF nucleotide-binding site opener antibody
see all
Images
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Western blot - Anti-Cytohesin 2 antibody (ab229994)All lanes : Anti-Cytohesin 2 antibody (ab229994) at 1/1000 dilution
Lane 1 : U-251 MG (human brain glioma cell line) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : A549 (human lung carcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 47 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytohesin 2 antibody (ab229994)Paraffin-embedded human ovarian cancer tissue stained for Cytohesin 2 using ab229994 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytohesin 2 antibody (ab229994)Paraffin-embedded human colon cancer tissue stained for Cytohesin 2 using ab229994 at 1/100 dilution in immunohistochemical analysis.
Datasheets and documents
References
ab229994 has not yet been referenced specifically in any publications.
