Product nameCytohesin 2 overexpression 293T lysate (whole cell)
ab94253 is a 293T cell transfected lysate in which Human Cytohesin 2 has been transiently over-expressed using a pCMV-Cytohesin 2 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
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BackgroundFunction: Acts as a guanine-nucleotide exchange factor (GEF). Promotes guanine-nucleotide exchange on ARF1, ARF3 and ARF6. Promotes the activation of ARF factors through replacement of GDP with GTP. The cell membrane form, in association with ARL4 proteins, recruits ARF6 to the plasma membrane. Tissue specificity: Ubiquitous. Similarity: Contains 1 PH domain. Contains 1 SEC7 domain. Domain: The PH domain is necessary and sufficient for plasma membrane relocalization.
Our Abpromise guarantee covers the use of ab94253 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94253 at 15μg/lane on an SDS-PAGE gel
All lanes : Anti-Cytohesin 2 antibody (ab56510) at 1/500 dilution
Lane 1 :
Cytohesin 2 overexpression 293T lysate (whole cell) (ab94253)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94253 has not yet been referenced specifically in any publications.