Key features and details
- Mouse monoclonal [34(beta)E12] to Cytokeratin 1 + 5 + 10+ 14
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Cytokeratin 1 + 5 + 10+ 14 antibody [34(beta)E12]
DescriptionMouse monoclonal [34(beta)E12] to Cytokeratin 1 + 5 + 10+ 14
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Tissue, cells or virus corresponding to Human Cytokeratin 1 + 5 + 10+ 14. Keratin solubilized from Human stratum corneum.
- ab195381 reacts with all squamous and ductal epithelium as well as carcinomas such as squamous cell carcinoma and adenocarcinoma of ductal origin (e.g., breast, pancreas) but does not react with sarcoma, melanoma or lymphoma. ab195381 reacts with benign small-acinar lesions of the prostate and has been shown to be useful as an adjunct in the identification of prostate carcinoma.
ab195381 is specific for "high molecular weight" cytokeratins 1, 5, 10 and 14, corresponding to molecular weights 68, 58, 56.5 and 50 kDa, respectively, which are characteristically found in "complex" epithelium.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: 1% BSA, 98% PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab195381 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
For optimal results, fixation in alcohol-based fixatives, such as methacarn, is recommended. Pretreatment with proteolytic enzymes such as trypsin may be required when formalin-fixed sections are to be stained. For optimal staining of prostate tissue higher concentrations of protease and longer incubation times are recommended, e.g., 0.5% trypsin at 37°C for 2 to 4 hours.
FunctionMay regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.
Tissue specificityThe source of this protein is neonatal foreskin. The 67-kDa type II keratins are expressed in terminally differentiating epidermis.
Involvement in diseaseEpidermolytic hyperkeratosis
Ichthyosis hystrix, Curth-Macklin type
Keratoderma, palmoplantar, non-epidermolytic
Ichthyosis annular epidermolytic
Keratoderma, palmoplantar, striate 3
Sequence similaritiesBelongs to the intermediate filament family.
modificationsUndergoes deimination of some arginine residues (citrullination).
Cellular localizationCell membrane. Located on plasma membrane of neuroblastoma NMB7 cells.
- Information by UniProt
- 67 kDa cytokeratin antibody
- CK 1 antibody
- CK 10 antibody
ab195381 has not yet been referenced specifically in any publications.