Overview

  • Product name

    Anti-Cytokeratin 1 + 5 + 10+ 14 antibody [34(beta)E12]
  • Description

    Mouse monoclonal [34(beta)E12] to Cytokeratin 1 + 5 + 10+ 14
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human Cytokeratin 1 + 5 + 10+ 14. Keratin solubilized from Human stratum corneum.

  • Positive control

    • ab195381 reacts with all squamous and ductal epithelium as well as carcinomas such as squamous cell carcinoma and adenocarcinoma of ductal origin (e.g., breast, pancreas) but does not react with sarcoma, melanoma or lymphoma. ab195381 reacts with benign small-acinar lesions of the prostate and has been shown to be useful as an adjunct in the identification of prostate carcinoma.
  • General notes

    ab195381 is specific for "high molecular weight" cytokeratins 1, 5, 10 and 14, corresponding to molecular weights 68, 58, 56.5 and 50 kDa, respectively, which are characteristically found in "complex" epithelium.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituents: 1% BSA, 98% PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    34(beta)E12
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab195381 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20.

For optimal results, fixation in alcohol-based fixatives, such as methacarn, is recommended. Pretreatment with proteolytic enzymes such as trypsin may be required when formalin-fixed sections are to be stained. For optimal staining of prostate tissue higher concentrations of protease and longer incubation times are recommended, e.g., 0.5% trypsin at 37°C for 2 to 4 hours.

WB 1/20 - 1/50.

Target

  • Function

    May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.
  • Tissue specificity

    The source of this protein is neonatal foreskin. The 67-kDa type II keratins are expressed in terminally differentiating epidermis.
  • Involvement in disease

    Epidermolytic hyperkeratosis
    Ichthyosis hystrix, Curth-Macklin type
    Keratoderma, palmoplantar, non-epidermolytic
    Ichthyosis annular epidermolytic
    Keratoderma, palmoplantar, striate 3
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Post-translational
    modifications

    Undergoes deimination of some arginine residues (citrullination).
  • Cellular localization

    Cell membrane. Located on plasma membrane of neuroblastoma NMB7 cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • 67 kDa cytokeratin antibody
    • CK 1 antibody
    • CK 10 antibody
    • CK 14 antibody
    • CK 5 antibody
    • CK-1 antibody
    • Cytokeratin-1 antibody
    • Hair alpha protein antibody
    • K1 antibody
    • K10 antibody
    • K14 antibody
    • K2C1_HUMAN antibody
    • K5 antibody
    • Keratin 1 antibody
    • Keratin 10 antibody
    • Keratin 14 antibody
    • Keratin 5 antibody
    • Keratin antibody
    • Keratin type I cytoskeletal 10 antibody
    • Keratin type I cytoskeletal 14 antibody
    • Keratin type II cytoskeletal 1 antibody
    • Keratin type II cytoskeletal 5 antibody
    • Keratin-1 antibody
    • Krt1 antibody
    • KRT10 antibody
    • KRT14 antibody
    • KRT5 antibody
    • type II cytoskeletal 1 antibody
    • Type-II keratin Kb1 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling Cytokeratin 1 + 5 + 10+ 14 with ab195381 at a 1/20 dilution.

References

ab195381 has not yet been referenced specifically in any publications.

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