Overview

  • Product name

    Anti-Cytokeratin 1 - 8 antibody [AE3], prediluted
  • Description

    Mouse monoclonal [AE3] to Cytokeratin 1 - 8, prediluted
  • Host species

    Mouse
  • Specificity

    ab32455 reacts with most of the Basic (Type II) keratins. Including epidermal cell keratin of 86 and 58kD and skin at 58kD, 65kD and 67kD. Broad keratin reactivity is found on human epidermal cell, oesophagus and cornea. Since each epithelium contains at least one basic keratin, AE3 is broadly reactive and stains positively almost all epithelia and their neoplasms.
  • Tested applications

    Suitable for: IHC-FoFr, IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Rabbit, Chicken, Cow, Fish, Monkey, Macaque monkey
  • Immunogen

    Tissue/ cell preparation - Human epidermal cells.

  • Positive control

    • Human epidermal cells, oesophagus, cornea.

Properties

Applications

Our Abpromise guarantee covers the use of ab34255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr
IHC-P
IHC-Fr
  • Application notes
    IHC-Fr: Use neat
    IHC-FoFr: Use neat.
    IHC-P: Use neat. This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    References

    This product has been referenced in:

    • Tsubura A  et al. Immunophenotypic difference of keratin expression in normal mammary glandular cells from five different species. Acta Anat (Basel) 140:287-93 (1991). Read more (PubMed: 1714223) »
    • Eichner R  et al. Classification of epidermal keratins according to their immunoreactivity, isoelectric point, and mode of expression. J Cell Biol 98:1388-96 (1984). Read more (PubMed: 6201491) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Chicken Tissue sections (chicken embryo epicardium)
    Specification
    chicken embryo epicardium
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0,2% Triton X-100 in PBS, 10'
    Blocking step
    Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 37°C

    Mrs. Elena Astanina

    Verified customer

    Submitted Feb 20 2008

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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