Anti-Cytokeratin 10 + 13 antibody [DE-K13] (ab9383)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-Cytokeratin 10 + 13 antibody [DE-K13]
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DescriptionMouse monoclonal [DE-K13] to Cytokeratin 10 + 13
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Host speciesMouse
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SpecificityThis antibody reacts with cytokeratin 10 and 13 in fixed cells and frozen tissue, but exclusively with cytokeratin 13 in formalin fixed paraffin embedded tissue.
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Tested applicationsSuitable for: Flow Cyt, ICC, IHC-Fr, WB, IHC-Pmore details
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Species reactivityReacts with: Cat, Dog, Human, Zebrafish
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Immunogen
Cytoskeletal preparation extracted from human ectocervical epithelium
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberDE-K13
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MyelomaSp2/0
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IsotypeIgG2a
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Light chain typekappa
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab9383 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/100 - 1/200. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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| ICC | Use at an assay dependent concentration. Reacts with cytokeratin 10 and 13 in fixed cells. | |
| IHC-Fr | Use at an assay dependent concentration. Reacts with cytokeratin 10 and 13 in frozen tissues. | |
| WB | 1/100 - 1/1000. | |
| IHC-P | 1/100 - 1/200. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. Reacts exclusively with cytokeratin 13 in formalin-fixed, paraffin-embedded tissues. |
Target
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Tissue specificitySeen in all suprabasal cell layers including stratum corneum.
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Involvement in diseaseEpidermolytic hyperkeratosis
Ichthyosis annular epidermolytic
Erythroderma, ichthyosiform, congenital reticular -
Sequence similaritiesBelongs to the intermediate filament family.
- Information by UniProt
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Database links
- Entrez Gene: 3858 Human
- Entrez Gene: 3860 Human
- Omim: 148065 Human
- Omim: 148080 Human
- SwissProt: P13645 Human
- SwissProt: P13646 Human
- Unigene: 463032 Human
- Unigene: 99936 Human
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Alternative names
- CK-10 antibody
- CK10 antibody
- CK13 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 + 13 antibody [DE-K13] (ab9383)](https://www.abcam.com/ps/products/9/ab9383/Images/Cytokeratin-10-13-Primary-antibodies-ab9383-8.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 + 13 antibody [DE-K13] (ab9383)This image is courtesy of an anonymous Abreview.ab9383 staining Cytokeratin 10 + 13 in human urogenital tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed in paraformaldehyde and a heat mediated antigen retrieval step was performed using sodium citrate buffer. Samples were then blocked using 5% serum for 1 hour then incubated with ab9383 at a 1/100 dilution for 2 hours at 37°C. The secondary used was a Cy3 conjugated donkey anti-mouse monoclonal used at a 1/500 dilution. -
![Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 10 + 13 antibody [DE-K13] (ab9383)](https://www.abcam.com/ps/products/9/ab9383/Images/Cytokeratin-10-13-Primary-antibodies-ab9383-9.jpg)
Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 10 + 13 antibody [DE-K13] (ab9383)Immunofluorescence staining of a 7 days old zebrafish embryo
Protocols
References
This product has been referenced in:
- Ganor Y et al. The adult penile urethra is a novel entry site for HIV-1 that preferentially targets resident urethral macrophages. Mucosal Immunol : (2012). Human . Read more (PubMed: 23187317) »
