Anti-Cytokeratin 10 + 13 antibody [DE-K13] (ab9383)

Reviews (2)Q&A (1)References (1)

Overview

  • Product name

    Anti-Cytokeratin 10 + 13 antibody [DE-K13]

  • Description

    Mouse monoclonal [DE-K13] to Cytokeratin 10 + 13

  • Host species

    Mouse

  • Specificity

    This antibody reacts with cytokeratin 10 and 13 in fixed cells and frozen tissue, but exclusively with cytokeratin 13 in formalin fixed paraffin embedded tissue.

  • Tested applications

    Suitable for: Flow Cyt, ICC, IHC-Fr, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Cat, Dog, Human, Zebrafish

  • Immunogen

    Cytoskeletal preparation extracted from human ectocervical epithelium

Properties

Applications

Our Abpromise guarantee covers the use of ab9383 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100 - 1/200.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 
ICC Use at an assay dependent concentration. Reacts with cytokeratin 10 and 13 in fixed cells.
IHC-Fr Use at an assay dependent concentration. Reacts with cytokeratin 10 and 13 in frozen tissues.
WB 1/100 - 1/1000.
IHC-P 1/100 - 1/200. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. Reacts exclusively with cytokeratin 13 in formalin-fixed, paraffin-embedded tissues.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 + 13 antibody [DE-K13] (ab9383)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 + 13 antibody [DE-K13] (ab9383)This image is courtesy of an anonymous Abreview.
    ab9383 staining Cytokeratin 10 + 13 in human urogenital tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in paraformaldehyde and a heat mediated antigen retrieval step was performed using sodium citrate buffer. Samples were then blocked using 5% serum for 1 hour then incubated with ab9383 at a 1/100 dilution for 2 hours at 37°C. The secondary used was a Cy3 conjugated donkey anti-mouse monoclonal used at a 1/500 dilution.

    See Abreview

  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 10 + 13 antibody [DE-K13] (ab9383)
    Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 10 + 13 antibody [DE-K13] (ab9383)
    Immunofluorescence staining of a 7 days old zebrafish embryo

References

This product has been referenced in:

  • Ganor Y  et al. The adult penile urethra is a novel entry site for HIV-1 that preferentially targets resident urethral macrophages. Mucosal Immunol : (2012). Human . Read more (PubMed: 23187317) »
See 1 Publication for this product

Publishing research using ab9383? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-Cytokeratin 10 + 13 antibody [DE-K13]

 Average
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Monkey Tissue sections (skin)
Specification
skin
Permeabilization
No
Fixative
Acetone
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Abcam user community

Verified customer

Submitted Jul 09 2013

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cytokeratin 10 + 13 antibody [DE-K13]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human Urogenital)
Specification
Human Urogenital
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Read More

Abcam user community

Verified customer

Submitted Nov 01 2011

Question

Je vous remercie d'abord votre aide pour me procéder une offre si intéressant Comme prévu, j'ai réussi à prendre les images en IF avec cet anticorps chez Abcam, mais je ne peux pas soumettre l'Abreview même si j'ai déjà rempli toute la questionnaire.   pourriez-vous me dire comment je pourrais faire le finir (soumission)?   bien cordialement,

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Answer

Merci pour votre Abreview. J'ai résolu le problème. Le numéro de lot n'était pas correct (un signe - de trop entre GR et le numéro). Votre Abreview est maintenant publiée et le code promotionnel est activé. Merci encore de votre collaboration.

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