Anti-Cytokeratin 10 antibody [DE-K10] - Cytoskeleton Marker (ab9026)

Reviews (4)Q&A (3)References (30)
Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 10 antibody [DE-K10] - Cytoskeleton Marker (ab9026)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [DE-K10] - Cytoskeleton Marker (ab9026)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [DE-K10] - Cytoskeleton Marker (ab9026)

Key features and details

  • Mouse monoclonal [DE-K10] to Cytokeratin 10 - Cytoskeleton Marker
  • Suitable for: IHC-Fr, IHC-P
  • Reacts with: Dog, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Cytokeratin 10 antibody [DE-K10] - Cytoskeleton Marker
    See all Cytokeratin 10 primary antibodies

  • Description

    Mouse monoclonal [DE-K10] to Cytokeratin 10 - Cytoskeleton Marker

  • Host species

    Mouse

  • Specificity

    Reacts with keratinizing stratified epithelia and in differentiated areas of highly differentiated squamous cell carcinomas.

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Dog
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Cytoskeletal preparation extracted from human epidermis

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein G purified

  • Clonality

    Monoclonal

  • Clone number

    DE-K10

  • Myeloma

    Sp2/0

  • Isotype

    IgG1

  • Light chain type

    kappa

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab9026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-Fr
Dog
IHC-P
Human
Application Abreviews Notes
IHC-Fr
Use at an assay dependent concentration.
IHC-P (3)
1/100.
Notes
IHC-Fr
Use at an assay dependent concentration.
IHC-P
1/100.

Target

  • Tissue specificity

    Seen in all suprabasal cell layers including stratum corneum.

  • Involvement in disease

    Defects in KRT10 are a cause of bullous congenital ichthyosiform erythroderma (BCIE) [MIM:113800]; also known as epidermolytic hyperkeratosis (EHK) or bullous erythroderma ichthyosiformis congenita of Brocq. BCIE is an autosomal dominant skin disorder characterized by widespread blistering and an ichthyotic erythroderma at birth that persist into adulthood. Histologically there is a diffuse epidermolytic degeneration in the lower spinous layer of the epidermis. Within a few weeks from birth, erythroderma and blister formation diminish and hyperkeratoses develop.
    Defects in KRT10 are a cause of ichthyosis annular epidermolytic (AEI) [MIM:607602]; also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI is a skin disorder resembling bullous congenital ichthyosiform erythroderma. Affected individuals present with bullous ichthyosis in early childhood and hyperkeratotic lichenified plaques in the flexural areas and extensor surfaces at later ages. The feature that distinguishes AEI from BCIE is dramatic episodes of flares of annular polycyclic plaques with scale, which coalesce to involve most of the body surface and can persist for several weeks or even months.

  • Sequence similarities

    Belongs to the intermediate filament family.
  • Information by UniProt

  • Database links

    • Alternative names

      • BCIE antibody
      • BIE antibody
      • CK 10 antibody
      • CK-10 antibody
      • Cytokeratin-10 antibody
      • EHK antibody
      • K10 antibody
      • K1C10_HUMAN antibody
      • Keratin 10 antibody
      • Keratin 10 type I antibody
      • Keratin antibody
      • Keratin type i cytoskeletal 10 antibody
      • Keratin type I cytoskeletal 59 kDa antibody
      • Keratin-10 antibody
      • Keratin10 antibody
      • KPP antibody
      • KRT10 antibody
      • type I cytoskeletal 10 antibody
      see all

    Images

    • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 10 antibody [DE-K10] - Cytoskeleton Marker (ab9026)
      Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 10 antibody [DE-K10] - Cytoskeleton Marker (ab9026)

      IHC image on a frozen section of  dog skin showing its strong reactivity in the keratinizing epidermal cells.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [DE-K10] - Cytoskeleton Marker (ab9026)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [DE-K10] - Cytoskeleton Marker (ab9026)
      IHC image of ab9026 staining in Human cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9026, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [DE-K10] - Cytoskeleton Marker (ab9026)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [DE-K10] - Cytoskeleton Marker (ab9026)Image from Reinheckel T et al, J Cell Sci. 2005 Aug 1;118(Pt 15):3387-95, Fig 3. DOI 10.1242/?jcs.02469
      Mesenchymal-epidermal interactions assessed by heterologous organotypic co-cultures (OTC). Heterologous OTC consisting of mice deficient for cathepsin L +/+ fibroblasts in collagen type I gels topped by normal human primary keratinocytes were grown air-exposed for 7 days. Paraffin sections were stained in haematoxylin and eosin (HE) or by immunohistochemistry for the proliferation marker Ki67 (Ki67, brown nuclear staining) and the differentiation markers cytokeratin 10 (K10, ab9026 1/100 dilution, brown staining) and transglutaminase (TG, brown staining). Bar 100 µm.

    References (30)

    Publishing research using ab9026? Please let us know so that we can cite the reference in this datasheet.

    ab9026 has been referenced in 30 publications.

    • Leng L  et al. Both Wnt signaling and epidermal stem cell-derived extracellular vesicles are involved in epidermal cell growth. Stem Cell Res Ther 11:415 (2020). PubMed: 32967725
    • Changarathil G  et al. Wild-type and SAMP8 mice show age-dependent changes in distinct stem cell compartments of the interfollicular epidermis. PLoS One 14:e0215908 (2019). PubMed: 31091266
    • Lämmermann I  et al. Blocking negative effects of senescence in human skin fibroblasts with a plant extract. NPJ Aging Mech Dis 4:4 (2018). PubMed: 29675264
    • Jørgensen E  et al. Normal microscopic anatomy of equine body and limb skin: A morphological and immunohistochemical study. Ann Anat 218:205-212 (2018). PubMed: 29730469
    • Zhou Z  et al. microRNA-203 Modulates Wound Healing and Scar Formation via Suppressing Hes1 Expression in Epidermal Stem Cells. Cell Physiol Biochem 49:2333-2347 (2018). PubMed: 30261495
    View all Publications for this product

    Customer reviews and Q&As

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    1-7 of 7 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cytokeratin 10 antibody [DE-K10] - Cytoskeleton Marker

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate buffer pH6
    Permeabilization
    No
    Specification
    skin
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    10% normal buffered formalin
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 09 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cytokeratin 10 antibody [DE-K10] - Cytoskeleton Marker

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Horse Tissue sections (SKIN)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TRIS-EDTA PH9
    Permeabilization
    Yes - 0,05% TX 100
    Specification
    SKIN
    Blocking step
    Serum as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Dr. Vincenzo Miragliotta

    Verified customer

    Submitted Jun 05 2017

    Western blot abreview for Anti-Cytokeratin 10 antibody [DE-K10] - Cytoskeleton Marker

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Pig Tissue lysate - whole (skin)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Treatment
    nurn wound with stem cell treatment
    Specification
    skin
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 20 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cytokeratin 10 antibody [DE-K10] - Cytoskeleton Marker

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 0.05% typsin
    Permeabilization
    No
    Specification
    skin
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 23 2015

    Question

    Follow up to previous correspondance: Please find attached a jpeg scan of a typical blot using the antibody, (ab9026) DK-E10. This spotting pattern has been consistently seen under a variety of conditions in an effort to optimize its use. In brief: Standard protocol 5ug protein/lane PVDF membrane Block 10% skimmed milk in TBST (0.1% Tween). Primary ab 1:5000 in 1% milk/TBST. 1 hour RT I have tried a wide variety of blocking agents, and incubated the primary ab overnight at 40C. I have also tried centrifuging the primary antibody and using the supernatant with change to the pattern. Detection system I have kept standard, and works well with all other antibodies on these lysates. I would be grateful if you could resolve this problem. Alternatively might it be worth trying an alternative clone, possibly RKSE-60, (ab9025). Best wishes,

    Read More
    Answer

    I have just received the following information from the supplier of ab9026: "1) Yes please, you can offer your customer a free (replacement) batch of our other keratin 10 ab9025. 2)The question is however, whether this will be of any help. Ab9026 seems to work perfectly well in the blotting, giving the proper mol. weight band, and I presume that in a one-dimensional SDS-gel your customer will find this one band (possible with a few fainter bands that represent the breakdown products of cytokeratin 10). In the two-dimensional gel, however, your customer finds multiple spots at the same mol. weight level. In our experience cytokeratin 10 is a difficult protein in iso-electric focussing, which I presume was used in the first dimension. You really need high (saturated; over 8M) urea in the sample buffer and in the focussing gel. Also, when using such high concentrations of urea, heating of the sample will give carbamylation of the proteins, resulting in exactly the “train” of spots as seen in your customers gel. I would therefore suggest your customer to take these items into consideration in preparing the sample and before preparing the gel/blot. " Would you like to give those suggestions a try or prefer a vial of ab9025 to be sent to you? I am away next week but my colleagues will be able to help you, please let us know if you need further assistance,

    Read More

    Question

    I have purchased cytokeratin 10 antibody ab9026 clone DK-E10 from you. I am attempting to probe for keratin 10 in lystates by western blotting from human oesophageal cell lines, previously detected by 2D gel electrophoresis and mass spectrometry. The antibody produces a single signal across the blot as expected at the predicted approximate molecular weight. However, the signal is a series of punctate blobs instead of a single line. The signal is also dotted around the blot. I have tried boiling and sonicating my lysate, with no improvemnet and 4 other antibodies work fine with this lysate. I have centrifuged the antibody diluted 1:500 in TBST in a microfuge at 13000rpm for ten minutes. This produces an improvement by removing the signal from around the blot. The signal around the predicted molecular weight is still in the form of punctate blobs! I would be grateful if you could provide me with a specific protocol, that will solve this problem for me, and if possible show me an image of a typical blot for this antibody. Alternatively, perhaps you could replace the antibody with another batch, or suggest an alternative clone. Best wishes,

    Read More
    Answer

    I'm sorry to hear you are having problem with ab9026. Spotting can be frequently caused by the blocking agent you use, I would therefore try filtering it and leaving on the membrane for 30min, then rinsing the membrane with TBST. Incubate the primary antibody in TBST at 4C and rinse the membrane well, then incubate the secondary in TBST. You may also have to try different blocking agents (milk, BSA, serum). Spotting can also be caused by the secondary antibody and the detection kit, you may wish to try other secondaries and a new detection kit. Please let me know if this helps and do not hesitate to contact us if you still have problems and we will send you a replacement.

    Read More

    Question

    I have used your cytokeratin 10 (clone DE-K10) successfully on normal dog tissue. I would like to use your antibody for Western blots on a group of dogs containing a K10 mutation resulting in a truncated protein. Would it be possible for you to tell us specifically what aa sequence the antibody binds to so that I know if my mutated protein would be expected to bind? Thanks

    Read More
    Answer

    The exact epitope of Ab9026 is not known. I'm afraid you just have to try and see if your truncated protein is still recognized by Ab9026.

    Read More

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