Recombinant Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1607IHCY] to Cytokeratin 10 - Cytoskeleton Marker
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker
See all Cytokeratin 10 primary antibodies -
Description
Rabbit monoclonal [EP1607IHCY] to Cytokeratin 10 - Cytoskeleton Marker -
Host species
Rabbit -
Specificity
Some customers have successfully used this antibody to staining Cytokeratin 10 in mouse tissue. Please see Abreviews for more details.
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Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Hacat and A431 cell lysates, human fetal, rat and mouse skin tissue lysates. IHC-P: Human skin and tonsil tissues and mouse skin tissue. ICC/IF: HACAT cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1607IHCY -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 647 Anti-Cytokeratin 10 antibody [EP1607IHCY] (ab194231)
- HRP Anti-Cytokeratin 10 antibody [EP1607IHCY] (ab194232)
- Anti-Cytokeratin 10 antibody [EP1607IHCY] - BSA and Azide free (ab220806)
- Anti-Cytokeratin 10 antibody [AE20] (ab237775)
- Anti-Cytokeratin 10 antibody [AE20] - BSA and Azide free (ab269570)
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
- Prestained Protein Ladder - Broad molecular weight (10 - 245 kDa) (ab116028)
- A-431 whole cell lysate (ab30132)
- Mouse skin tissue lysate - total protein (ab4025)
- Rat skin tissue lysate - total protein (ab4037)
- Mouse skin tissue lysate - total protein (0 days) (ab7269)
- Mouse skin tissue lysate (14 days) (ab7271)
- Mouse skin tissue lysate (7 days) (ab7273)
- A-431 whole cell lysate (ab7909)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76318 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (1) |
1/150.
For unpurified use at 1/100 - 1/250. |
WB |
1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
For unpurified use at 1/50000 - 1/100000. |
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IHC-P | (10) |
1/2500 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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ICC/IF
1/150. For unpurified use at 1/100 - 1/250. |
WB
1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). For unpurified use at 1/50000 - 1/100000. |
IHC-P
1/2500 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Tissue specificity
Seen in all suprabasal cell layers including stratum corneum. -
Involvement in disease
Defects in KRT10 are a cause of bullous congenital ichthyosiform erythroderma (BCIE) [MIM:113800]; also known as epidermolytic hyperkeratosis (EHK) or bullous erythroderma ichthyosiformis congenita of Brocq. BCIE is an autosomal dominant skin disorder characterized by widespread blistering and an ichthyotic erythroderma at birth that persist into adulthood. Histologically there is a diffuse epidermolytic degeneration in the lower spinous layer of the epidermis. Within a few weeks from birth, erythroderma and blister formation diminish and hyperkeratoses develop.
Defects in KRT10 are a cause of ichthyosis annular epidermolytic (AEI) [MIM:607602]; also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI is a skin disorder resembling bullous congenital ichthyosiform erythroderma. Affected individuals present with bullous ichthyosis in early childhood and hyperkeratotic lichenified plaques in the flexural areas and extensor surfaces at later ages. The feature that distinguishes AEI from BCIE is dramatic episodes of flares of annular polycyclic plaques with scale, which coalesce to involve most of the body surface and can persist for several weeks or even months. -
Sequence similarities
Belongs to the intermediate filament family. - Information by UniProt
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Database links
- Entrez Gene: 3858 Human
- Entrez Gene: 16661 Mouse
- Entrez Gene: 450225 Rat
- Omim: 148080 Human
- SwissProt: P13645 Human
- SwissProt: P02535 Mouse
- SwissProt: Q6IFW6 Rat
- Unigene: 99936 Human
see all -
Alternative names
- BCIE antibody
- BIE antibody
- CK 10 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse skin tissue labelling Cytokeratin 10 with unpurified ab76318 at a 1/10000 dilution. The sections were subjected to heat mediated antigen retrieval. The sections were then blocked using 1% BSA for 10 mins at 21°C. ab76318 was incubated for 2 hours at 21°C. A biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody (1/250).
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Immunocytochemistry/Immunofluorescence analysis of HACAT cells labelling Cytokeratin 10 with purified ab76318 at 1/150. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/500) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.
Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).
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All lanes : Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318) at 1/10000 dilution (purified)
Lane 1 : HACAT cell lysate
Lane 2 : A431 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue labelling Cytokeratin 10 with unpurified ab76318 at a 1/6000 dilution. The sections were subjected to heat mediated antigen retrieval. The sections were then blocked using 1% BSA for 10 mins at 21°C. ab76318 was incubated for 2 hours at 21°C. A biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody (1/200).
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Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318) at 1/50000 dilution (purified) + Human fetal skin tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318) at 1/50000 dilution (purified) + Rat skin tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse skin tissue labelling Cytokeratin 10 with purified ab76318 at 1/5000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318) at 1/200000 dilution (purified) + Mouse skin tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling Cytokeratin 10 with purified ab76318 at 1/5000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318) at 1/100000 dilution + HaCat cell lysate at 10 µg
Secondary
HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human normal skin tissue labelling Cytokeratin 10 with unpurified ab76318.
Green - CK10, red - PI.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat skin tissue labelling Cytokeratin 10 with unpurified ab76318 at a 1/10000 dilution. The sections were subjected to heat mediated antigen retrieval. The sections were then blocked using 1% BSA for 10 mins at 21°C. ab76318 was incubated for 2 hours at 21°C. A biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody (1/250).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (106)
ab76318 has been referenced in 106 publications.
- Xu Q et al. Topical astilbin ameliorates imiquimod-induced psoriasis-like skin lesions in SKH-1 mice via suppression dendritic cell-Th17 inflammation axis. J Cell Mol Med 26:1281-1292 (2022). PubMed: 35023281
- Yokota M et al. Trehangelins ameliorate inflammation-induced skin senescence by suppressing the epidermal YAP-CCN1 axis. Sci Rep 12:952 (2022). PubMed: 35046484
- Guo Y et al. Hyperglycemia Induces Meibomian Gland Dysfunction. Invest Ophthalmol Vis Sci 63:30 (2022). PubMed: 35072689
- Kim K et al. An Interleukin-4 and Interleukin-13 Induced Atopic Dermatitis Human Skin Equivalent Model by a Skin-On-A-Chip. Int J Mol Sci 23:N/A (2022). PubMed: 35216228
- Hong Y et al. Malfunction of airway basal stem cells plays a crucial role in pathophysiology of tracheobronchopathia osteoplastica. Nat Commun 13:1309 (2022). PubMed: 35288560