Overview

  • Product name
    Anti-Cytokeratin 10 antibody [RKSE60]
    See all Cytokeratin 10 primary antibodies
  • Description
    Mouse monoclonal [RKSE60] to Cytokeratin 10
  • Host species
    Mouse
  • Specificity
    This antibody reacts exclusively with cytokeratin 10 which is present in keratinizing stratified epithelia and in differentiated areas of highly differentiated squamous cell carcinomas
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human, Pig
    Does not react with: Zebrafish
  • Immunogen

    Cytokeratins from the human epidermis.

  • Positive control
    • human keratinocytes

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    RKSE60
  • Myeloma
    Sp2/0
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100 - 1/200.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IHC-Fr Use at an assay dependent concentration.
WB 1/500 - 1/1000. (see reviews). Detects two bands of approximately 55 kDa (heterotetramer of two type I and two type II keratins).
ICC/IF Use at an assay dependent concentration.

Target

  • Tissue specificity
    Seen in all suprabasal cell layers including stratum corneum.
  • Involvement in disease
    Defects in KRT10 are a cause of bullous congenital ichthyosiform erythroderma (BCIE) [MIM:113800]; also known as epidermolytic hyperkeratosis (EHK) or bullous erythroderma ichthyosiformis congenita of Brocq. BCIE is an autosomal dominant skin disorder characterized by widespread blistering and an ichthyotic erythroderma at birth that persist into adulthood. Histologically there is a diffuse epidermolytic degeneration in the lower spinous layer of the epidermis. Within a few weeks from birth, erythroderma and blister formation diminish and hyperkeratoses develop.
    Defects in KRT10 are a cause of ichthyosis annular epidermolytic (AEI) [MIM:607602]; also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI is a skin disorder resembling bullous congenital ichthyosiform erythroderma. Affected individuals present with bullous ichthyosis in early childhood and hyperkeratotic lichenified plaques in the flexural areas and extensor surfaces at later ages. The feature that distinguishes AEI from BCIE is dramatic episodes of flares of annular polycyclic plaques with scale, which coalesce to involve most of the body surface and can persist for several weeks or even months.
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Information by UniProt
  • Database links
  • Alternative names
    • BCIE antibody
    • BIE antibody
    • CK 10 antibody
    • CK-10 antibody
    • Cytokeratin-10 antibody
    • EHK antibody
    • K10 antibody
    • K1C10_HUMAN antibody
    • Keratin 10 antibody
    • Keratin 10 type I antibody
    • Keratin antibody
    • Keratin type i cytoskeletal 10 antibody
    • Keratin type I cytoskeletal 59 kDa antibody
    • Keratin-10 antibody
    • Keratin10 antibody
    • KPP antibody
    • KRT10 antibody
    • type I cytoskeletal 10 antibody
    see all

Images

  • ab9025 staining Cytokeratin 10 in human skin tissue by Immunohistochemistry (Frozen sections). The tissue was acetone fixed and then blocked using 0.25% casein for 10 minutes at 20°C. Samples were then incubated with primary antibody at 1/1000 for 1 hour at 20°C. The secondary antibody used was a mouse anti-mouse monoclonal conjugated to Alexa Fluor® 488 (green) used at a 1/200 dilution.

    See Abreview

  • Immunohistochemistry on frozen section of swine skin: positive staining of the keratinizing layer
  • Immunofluorescence staining of a 7 days old zebrafish embryo

References

This product has been referenced in:
  • Qu Y  et al. The dual delivery of KGF and bFGF by collagen membrane to promote skin wound healing. J Tissue Eng Regen Med 12:1508-1518 (2018). Read more (PubMed: 29706001) »
  • Suzuki D  et al. Inhibition of TGF-ß signaling supports high proliferative potential of diverse p63(+) mouse epithelial progenitor cells in vitro. Sci Rep 7:6089 (2017). Read more (PubMed: 28729719) »
See all 12 Publications for this product

Customer reviews and Q&As

1-10 of 14 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Pig Tissue lysate - whole (skin)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Treatment
adipose-derived stem cell culture media
Specification
skin
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 29 2016

Application
Western blot
Sample
Pig Tissue lysate - whole (skin)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Treatment
burn wound with stem cell treatment
Specification
skin
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted May 20 2015

Answer

Vous avez testé l'anticorps anti-cytokeratin 10 et l'isotype en Western Blot. Avez-vous également utilisé l'anticorps secondaire seul?
Je suis étonné que la bande observée avec l'isotype se retrouve au même poids moléculaire que le primaire. Cette bande étant proche des 50 kDa, je me demande si ce n'est pas un marquage non-spécifique du secondaire. Un controle "no primary" permettrait de déterminer quel anticorps se fixe à ce niveau de la membrane.

Je vaiscontinuer de réfléchir à votre problème.

Bonne fin de journée et bon week-end.

Read More

Answer

Mercipour votre réponse ainsi que pour le questionnaire rempli et détaillé.

Il m'est toujours impossible de trouver une explication définitive à votre problème.
Est-ce que l'anticorps anti-K10 est également un monoclonal de souris IgG1 ?
Est-ce que ce même anticorps marque les controles négatifs (HEK) en cytométrie?
Quel controle isotype utilisez-vous avec cet anticorps?

J'espère pouvoir trouver rapidement une explication et une résolution à votre problème. N'hésitez pas à me communiquer votre opinion sur la question.

Read More

Answer

En plus de vos résultats obtenus en Western Blot, vous serait-il possible de remplir et nous retourner le questionnaire ci-joint?

Les informations sur le protocole utilisé nous sera utile pour tenter de comprendre le problème rencontré avec les 2 anticorps ab9025 et ab18448.

Merci.

Read More

Answer

Merci de nous avoir contactés.

Je vous envoie comme convenu un email auquel vous pourrez répondre et joindre vos résultats en Western Blot avec les anticorps ab9025 et ab18448.
Votre réponse me sera alors automatiquement attribuée.

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Question
Answer



Le laboratoire vient de nous confirmer que la chaine légère du clone [RKSE60] est de type kappa.

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Question
Answer

Thank you for your phone call. I found just the information in our records and am happy to confirm that the laboratory has obtained good results with this antibody at 1:100 – 1:200 for flow cytometry. I hope this information is helpful. Please do not hesitate to contact us again should you have any further question.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Skin)
Specification
Skin
Fixative
Acetone
Permeabilization
No
Blocking step
Casein as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: 20°C

Dr. Vaughan Feisst

Verified customer

Submitted Sep 17 2010

Answer

Thank you for contacting us for technical support. I do not advise the customer to use this antibody on mouse tissue in immunohistochemical methods as it is raised in mouse. Therefore the secondary anti-mouse antibody will bind to the whole of the tissue and give high non specific binding/high background. The antibody has not been tested in IHC-P, I would recommend diluting the antibody in PBS triton x100 (0.3%) at a range of concentrations to determine the optimal concentration to use (1:10-1:100), but I do not expect the antibody to work well in mouse sections as I explained above. Please let me know if you require further assistance,

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1-10 of 14 Abreviews or Q&A

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