Overview

  • Product name

    Anti-Cytokeratin 10 antibody [SP99] - C-terminal
    See all Cytokeratin 10 primary antibodies
  • Description

    Rabbit monoclonal [SP99] to Cytokeratin 10 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Dog
  • Immunogen

    Synthetic peptide within Human Cytokeratin 10 aa 550 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P13645

  • Positive control

    • Human skin, skin squamous cell carcinoma, cervical squamous cell carcinoma tissues IHC-Fr: Rat and mouse skin tissue
  • General notes

     

     

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.6
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from TCS by protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    SP99
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/50.
IHC-P 1/100.

Antigen retrieval not required

Target

  • Tissue specificity

    Seen in all suprabasal cell layers including stratum corneum.
  • Involvement in disease

    Defects in KRT10 are a cause of bullous congenital ichthyosiform erythroderma (BCIE) [MIM:113800]; also known as epidermolytic hyperkeratosis (EHK) or bullous erythroderma ichthyosiformis congenita of Brocq. BCIE is an autosomal dominant skin disorder characterized by widespread blistering and an ichthyotic erythroderma at birth that persist into adulthood. Histologically there is a diffuse epidermolytic degeneration in the lower spinous layer of the epidermis. Within a few weeks from birth, erythroderma and blister formation diminish and hyperkeratoses develop.
    Defects in KRT10 are a cause of ichthyosis annular epidermolytic (AEI) [MIM:607602]; also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI is a skin disorder resembling bullous congenital ichthyosiform erythroderma. Affected individuals present with bullous ichthyosis in early childhood and hyperkeratotic lichenified plaques in the flexural areas and extensor surfaces at later ages. The feature that distinguishes AEI from BCIE is dramatic episodes of flares of annular polycyclic plaques with scale, which coalesce to involve most of the body surface and can persist for several weeks or even months.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Information by UniProt
  • Database links

  • Alternative names

    • BCIE antibody
    • BIE antibody
    • CK 10 antibody
    • CK-10 antibody
    • Cytokeratin-10 antibody
    • EHK antibody
    • K10 antibody
    • K1C10_HUMAN antibody
    • Keratin 10 antibody
    • Keratin 10 type I antibody
    • Keratin antibody
    • Keratin type i cytoskeletal 10 antibody
    • Keratin type I cytoskeletal 59 kDa antibody
    • Keratin-10 antibody
    • Keratin10 antibody
    • KPP antibody
    • KRT10 antibody
    • type I cytoskeletal 10 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skin tissue sections labeling Cytokeratin 10 with ab183317 at 1/100 dilution (0.95 µg/ml). Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 1 (pH 6.0) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human skin, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab183317 for 10 mins at room temperature.

  • Immunohistochemistry (Frozen) analysis of rat skin tissue section labeling Cytokeratin 10 with purified ab183317 at 1/50 (1.9 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Frozen) analysis of mouse skin tissue section labeling Cytokeratin 10 with purified ab183317 at 1/50 (1.9 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemical analysis of Human skin labeling Cytokeratin 10 using ab183317 at a 1/100 dilution

  • Immunohistochemical analysis of Human skin labeling Cytokeratin 10 using ab183317 at a 1/100 dilution

  • Immunohistochemical analysis of Human skin squamous cell carcinoma labeling Cytokeratin 10 using ab183317 at a 1/100 dilution

  • Immunohistochemical analysis of Human cervical squamous cell carcinoma labeling Cytokeratin 10 using ab183317 at a 1/100 dilution.

References

ab183317 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Human Cell (skin)
Specification
skin
Permeabilization
No
Fixative
Formaldehyde

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Verified customer

Submitted Nov 17 2014

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