Anti-Cytokeratin 13 antibody (ab154346)
Key features and details
- Rabbit polyclonal to Cytokeratin 13
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cytokeratin 13 antibody
See all Cytokeratin 13 primary antibodies -
Description
Rabbit polyclonal to Cytokeratin 13 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 259-458 of Human Cytokeratin 13
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Positive control
- MCF7 whole cell lysate; MCF7 cells; CAL27 xenograft
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab154346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 50 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Tissue specificity
Expressed in some epidermal sweat gland ducts (at protein level) and in exocervix, esophagus and placenta. -
Involvement in disease
Defects in KRT13 are a cause of white sponge nevus of cannon (WSN) [MIM:193900]. WSN is a rare autosomal dominant disorder which predominantly affects non-cornified stratified squamous epithelia. Clinically, it is characterized by the presence of soft, white, and spongy plaques in the oral mucosa. The characteristic histopathologic features are epithelial thickening, parakeratosis, and vacuolization of the suprabasal layer of oral epithelial keratinocytes. Less frequently the mucous membranes of the nose, esophagus, genitalia and rectum are involved. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsO-glycosylated; glycans consist of single N-acetylglucosamine residues. - Information by UniProt
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Database links
- Entrez Gene: 3860 Human
- Omim: 148065 Human
- SwissProt: P13646 Human
- Unigene: 654550 Human
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Alternative names
- 47 kDa cytokeratin antibody
- CK-13 antibody
- CK13 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody (ab154346)Immunohistochemical analysis of paraffin-embedded CAL27 xenograft labeling Cytokeratin 13 with ab154346 at 1/500 dilution
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Western blot - Anti-Cytokeratin 13 antibody (ab154346)Anti-Cytokeratin 13 antibody (ab154346) at 1/5000 dilution + MCF7 whole cell lysate at 30 µg
Predicted band size: 50 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 13 antibody (ab154346)Immunofluorescence analysis of methanol-fixed MCF7 cells labeling Cytokeratin 13 with ab154346 at 1/200 dilution
Protocols
References (1)
ab154346 has been referenced in 1 publication.
- de Carvalho Dias K et al. Development and characterization of a 3D oral mucosa model as a tool for host-pathogen interactions. J Microbiol Methods 152:52-60 (2018). PubMed: 30017850
