Anti-Cytokeratin 13 antibody (ab154346)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-Cytokeratin 13 antibody
See all Cytokeratin 13 primary antibodies -
Description
Rabbit polyclonal to Cytokeratin 13 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 259-458 of Human Cytokeratin 13
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Positive control
- MCF7 whole cell lysate; MCF7 cells; CAL27 xenograft
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79% PBS, 20% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab154346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 50 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Tissue specificity
Expressed in some epidermal sweat gland ducts (at protein level) and in exocervix, esophagus and placenta. -
Involvement in disease
Defects in KRT13 are a cause of white sponge nevus of cannon (WSN) [MIM:193900]. WSN is a rare autosomal dominant disorder which predominantly affects non-cornified stratified squamous epithelia. Clinically, it is characterized by the presence of soft, white, and spongy plaques in the oral mucosa. The characteristic histopathologic features are epithelial thickening, parakeratosis, and vacuolization of the suprabasal layer of oral epithelial keratinocytes. Less frequently the mucous membranes of the nose, esophagus, genitalia and rectum are involved. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsO-glycosylated; glycans consist of single N-acetylglucosamine residues. - Information by UniProt
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Database links
- Entrez Gene: 3860 Human
- Omim: 148065 Human
- SwissProt: P13646 Human
- Unigene: 654550 Human
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Alternative names
- 47 kDa cytokeratin antibody
- CK-13 antibody
- CK13 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody (ab154346)Immunohistochemical analysis of paraffin-embedded CAL27 xenograft labeling Cytokeratin 13 with ab154346 at 1/500 dilution -
Western blot - Anti-Cytokeratin 13 antibody (ab154346)Anti-Cytokeratin 13 antibody (ab154346) at 1/5000 dilution + MCF7 whole cell lysate at 30 µg
Predicted band size: 50 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 13 antibody (ab154346)Immunofluorescence analysis of methanol-fixed MCF7 cells labeling Cytokeratin 13 with ab154346 at 1/200 dilution
Protocols
References
This product has been referenced in:
- de Carvalho Dias K et al. Development and characterization of a 3D oral mucosa model as a tool for host-pathogen interactions. J Microbiol Methods 152:52-60 (2018). Read more (PubMed: 30017850) »
