Anti-Cytokeratin 13 antibody (ab97327)
Key features and details
- Rabbit polyclonal to Cytokeratin 13
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cytokeratin 13 antibody
See all Cytokeratin 13 primary antibodies -
Description
Rabbit polyclonal to Cytokeratin 13 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Recombinant fragment corresponding to a region within amino acids 198-448 of Human Cytokeratin 13 (NP_705694).
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Positive control
- WB: HeLa whole cell lysate ICC/IF: A431 cells
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab97327 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 50 kDa. | |
| ICC/IF | 1/100 - 1/200. |
Target
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Tissue specificity
Expressed in some epidermal sweat gland ducts (at protein level) and in exocervix, esophagus and placenta. -
Involvement in disease
Defects in KRT13 are a cause of white sponge nevus of cannon (WSN) [MIM:193900]. WSN is a rare autosomal dominant disorder which predominantly affects non-cornified stratified squamous epithelia. Clinically, it is characterized by the presence of soft, white, and spongy plaques in the oral mucosa. The characteristic histopathologic features are epithelial thickening, parakeratosis, and vacuolization of the suprabasal layer of oral epithelial keratinocytes. Less frequently the mucous membranes of the nose, esophagus, genitalia and rectum are involved. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsO-glycosylated; glycans consist of single N-acetylglucosamine residues. - Information by UniProt
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Database links
- Entrez Gene: 3860 Human
- Entrez Gene: 16663 Mouse
- Omim: 148065 Human
- SwissProt: P13646 Human
- SwissProt: P08730 Mouse
- Unigene: 654550 Human
- Unigene: 4646 Mouse
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Alternative names
- 47 kDa cytokeratin antibody
- CK-13 antibody
- CK13 antibody
see all
Images
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Western blot - Anti-Cytokeratin 13 antibody (ab97327)Anti-Cytokeratin 13 antibody (ab97327) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 50 kDa
10% SDS-PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 13 antibody (ab97327)ab97327, at a 1/200 dilution, staining Cytokeratin 13 in paraformaldehyde fixed A431 cells by Immunofluorescence. Lower image is merged with DNA probe.
Protocols
References (1)
ab97327 has been referenced in 1 publication.
- Imaizumi M et al. In vitro epithelial differentiation of human induced pluripotent stem cells for vocal fold tissue engineering. Ann Otol Rhinol Laryngol 122:737-47 (2013). PubMed: 24592576
