Anti-Cytokeratin 13 antibody [AE8] (ab16112)
- Datasheet
- References (13)
- Protocols
Overview
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Product nameAnti-Cytokeratin 13 antibody [AE8]
See all Cytokeratin 13 primary antibodies -
DescriptionMouse monoclonal [AE8] to Cytokeratin 13
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Host speciesMouse
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Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rabbit, Human
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Immunogen
Rabbit esophageal keratins.
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General notes
This antibody is specific for Cytokeratin 13, which is a marker for oesophageal type differentiation which is expressed by various internal stratified epithelia.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
PurityProtein G purified
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Primary antibody notesThis antibody is specific for Cytokeratin 13, which is a marker for oesophageal type differentiation which is expressed by various internal stratified epithelia.
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ClonalityMonoclonal
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Clone numberAE8
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MyelomaP3-X63 Ag8.3
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunohistochemistry kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab16112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. PubMed: 25076852 | |
| WB | 1/1000 - 1/3000. Predicted molecular weight: 50 kDa. | |
| IHC-P | Use at an assay dependent concentration. |
Target
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Tissue specificityExpressed in some epidermal sweat gland ducts (at protein level) and in exocervix, esophagus and placenta.
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Involvement in diseaseDefects in KRT13 are a cause of white sponge nevus of cannon (WSN) [MIM:193900]. WSN is a rare autosomal dominant disorder which predominantly affects non-cornified stratified squamous epithelia. Clinically, it is characterized by the presence of soft, white, and spongy plaques in the oral mucosa. The characteristic histopathologic features are epithelial thickening, parakeratosis, and vacuolization of the suprabasal layer of oral epithelial keratinocytes. Less frequently the mucous membranes of the nose, esophagus, genitalia and rectum are involved.
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Sequence similaritiesBelongs to the intermediate filament family.
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Post-translational
modificationsO-glycosylated; glycans consist of single N-acetylglucosamine residues. - Information by UniProt
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Database links
- Entrez Gene: 3860 Human
- Entrez Gene: 16663 Mouse
- Omim: 148065 Human
- SwissProt: P13646 Human
- SwissProt: P08730 Mouse
- Unigene: 654550 Human
- Unigene: 4646 Mouse
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Alternative names
- 47 kDa cytokeratin antibody
- CK-13 antibody
- CK13 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [AE8] (ab16112)](https://www.abcam.com/ps/products/16/ab16112/Images/ab16112-210106-ab16112ihcp.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [AE8] (ab16112)This image is courtesy of an anonymous Abreviewab16112 staining Cytokeratin 13 in Human pharynx tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and antigen retrieval was by heat mediation in Tris pH9. Samples were incubated with primary antibody (undiluted) for 1 hour at 20°C. An undiluted HRP-conjugated Goat anti-mouse IgG polyclonal was used as the secondary antibody.
Protocols
References
This product has been referenced in:
- Kobayashi Y et al. Generation of a TALEN-mediated, p63 knock-in in human induced pluripotent stem cells. Stem Cell Res 25:256-265 (2017). IF ; Human . Read more (PubMed: 29179035) »
- Okuda Y et al. Wnt signaling as a possible promoting factor of cell differentiation in pleomorphic adenomas. Int J Med Sci 11:971-8 (2014). ICC/IF ; Human . Read more (PubMed: 25076852) »
