Recombinant
RabMAb

Recombinant Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)

Rabbit recombinant monoclonal Cytokeratin 13 antibody [EPR3671]. Validated in WB, IHC, ICC, Flow Cyt and tested in Mouse, Human. Cited in 12 publication(s). Independently reviewed in 1 review(s).

Overview

  • Product name

    Anti-Cytokeratin 13 antibody [EPR3671]
    See all Cytokeratin 13 primary antibodies
  • Description

    Rabbit monoclonal [EPR3671] to Cytokeratin 13
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, WB, ICC, IHC-P, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Cytokeratin 13 aa 100-200. The exact sequence is proprietary.
    (Peptide available as ab180923)

  • Positive control

    • WB: A431, HACAT or 293T cell lysate. IHC: transitional cell urinary bladder carcinoma tissue. FC: A431 cells
  • General notes

     

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab92551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB 1/100000 - 1/500000. Predicted molecular weight: 50 kDa.Can be blocked with Cytokeratin 13 peptide (ab180923).
ICC 1/100 - 1/250.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Flow Cyt 1/20.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Tissue specificity

      Expressed in some epidermal sweat gland ducts (at protein level) and in exocervix, esophagus and placenta.
    • Involvement in disease

      Defects in KRT13 are a cause of white sponge nevus of cannon (WSN) [MIM:193900]. WSN is a rare autosomal dominant disorder which predominantly affects non-cornified stratified squamous epithelia. Clinically, it is characterized by the presence of soft, white, and spongy plaques in the oral mucosa. The characteristic histopathologic features are epithelial thickening, parakeratosis, and vacuolization of the suprabasal layer of oral epithelial keratinocytes. Less frequently the mucous membranes of the nose, esophagus, genitalia and rectum are involved.
    • Sequence similarities

      Belongs to the intermediate filament family.
    • Post-translational
      modifications

      O-glycosylated; glycans consist of single N-acetylglucosamine residues.
    • Information by UniProt
    • Database links

    • Alternative names

      • 47 kDa cytokeratin antibody
      • CK-13 antibody
      • CK13 antibody
      • Cytokeratin 13 antibody
      • Cytokeratin-13 antibody
      • K13 antibody
      • K1C13_HUMAN antibody
      • Ka13 antibody
      • Keratin 13 antibody
      • Keratin antibody
      • keratin type I cytoskeletal 13 antibody
      • Keratin-13 antibody
      • Krt-1.13 antibody
      • Krt1-13 antibody
      • KRT13 antibody
      • MGC161462 antibody
      • MGC3781 antibody
      • type I cytoskeletal 13 antibody
      • Type I keratin Ka13 antibody
      • WSN2 antibody
      see all

    Images

    • ab92551 at 1/100 dilution staining Cytokeratin 13 in formalin-fixed, paraffin-embedded transitional cell usrinary bladder carcinoma tissue by immunohistochemistry. Detection: DAB staining.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • All lanes : Anti-Cytokeratin 13 antibody [EPR3671] (ab92551) at 1/100000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : HACAT cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Lanes 1-2 : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
      Lane 3 : HRP labelled goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size: 50 kDa

    • ab92551 staining Cytokeratin 13 in Human vaginal epithelium tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.2% Triton in PBS and blocked with 1% fish skin gelatin for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. An Alexa Fluor®594-conjugated Goat anti-rabbit polyclonal (1/1000) was used as the secondary antibody.
    • Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labeling Cytokeratin 13 with purified ab92551 at 1/20 dilution (red). The secondary antibody was Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

    • ab92551 showing positive staining in Cervical carcinoma tissue.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • ab92551 showing positive staining in Normal tonsil squamous cells tissue.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • ab92551 showing negative staining in Glioma tissue.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • ab92551 showing negative staining in Breast carcinoma tissue.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Wang YC  et al. Notch Signaling Pathway Is Inhibited in the Development of Barrett's Esophagus: An In Vivo and In Vitro Study. Can J Gastroenterol Hepatol 2018:4149317 (2018). Read more (PubMed: 29785394) »
    • Ustiyan V  et al. FOXF1 transcription factor promotes lung morphogenesis by inducing cellular proliferation in fetal lung mesenchyme. Dev Biol 443:50-63 (2018). Read more (PubMed: 30153454) »
    See all 13 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Question
    Answer

    Thank you for contacting us.

    This product because it is IVD has not been imported to Abcam website. Epitomics still sell this product via their website. Please contact them at the following email address; mailto:IVDorders@epitomics.com

    or contact by filling the form using the link;

    http://www.epitomics.com/customer_interactions/ivd_contact/

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (vaginal epithelium)
    Specification
    vaginal epithelium
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.2% Triton in PBS
    Blocking step
    (agent) for 30 minute(s) · Concentration: 1% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Oct 19 2011

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