Recombinant
RabMAb

Recombinant Anti-Cytokeratin 13 antibody [EPR3671] - BSA and Azide free (ab239918)

Overview

  • Product name

    Anti-Cytokeratin 13 antibody [EPR3671] - BSA and Azide free
    See all Cytokeratin 13 primary antibodies
  • Description

    Rabbit monoclonal [EPR3671] to Cytokeratin 13 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, ICC, IHC-P, IHC-Fr, WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Cytokeratin 13 aa 100-200. The exact sequence is proprietary.

  • General notes

    ab239918 is the carrier-free version of ab92551 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab239918 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab239918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 50 kDa.Can be blocked with Cytokeratin 13 peptide (ab180923).
  • Application notes
    Is unsuitable for IP.
  • Target

    • Tissue specificity

      Expressed in some epidermal sweat gland ducts (at protein level) and in exocervix, esophagus and placenta.
    • Involvement in disease

      Defects in KRT13 are a cause of white sponge nevus of cannon (WSN) [MIM:193900]. WSN is a rare autosomal dominant disorder which predominantly affects non-cornified stratified squamous epithelia. Clinically, it is characterized by the presence of soft, white, and spongy plaques in the oral mucosa. The characteristic histopathologic features are epithelial thickening, parakeratosis, and vacuolization of the suprabasal layer of oral epithelial keratinocytes. Less frequently the mucous membranes of the nose, esophagus, genitalia and rectum are involved.
    • Sequence similarities

      Belongs to the intermediate filament family.
    • Post-translational
      modifications

      O-glycosylated; glycans consist of single N-acetylglucosamine residues.
    • Information by UniProt
    • Database links

    • Alternative names

      • 47 kDa cytokeratin antibody
      • CK-13 antibody
      • CK13 antibody
      • Cytokeratin 13 antibody
      • Cytokeratin-13 antibody
      • K13 antibody
      • K1C13_HUMAN antibody
      • Ka13 antibody
      • Keratin 13 antibody
      • Keratin antibody
      • keratin type I cytoskeletal 13 antibody
      • Keratin-13 antibody
      • Krt-1.13 antibody
      • Krt1-13 antibody
      • KRT13 antibody
      • MGC161462 antibody
      • MGC3781 antibody
      • type I cytoskeletal 13 antibody
      • Type I keratin Ka13 antibody
      • WSN2 antibody
      see all

    Images

    • ab92551 at 1/100 dilution staining Cytokeratin 13 in formalin-fixed, paraffin-embedded transitional cell usrinary bladder carcinoma tissue by immunohistochemistry. Detection: DAB staining.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92551).

    • Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labeling Cytokeratin 13 with purified ab92551 at 1/20 dilution (red). The secondary antibody was Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92551).

    • ab92551 staining Cytokeratin 13 in Human vaginal epithelium tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.2% Triton in PBS and blocked with 1% fish skin gelatin for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. An Alexa Fluor®594-conjugated Goat anti-rabbit polyclonal (1/1000) was used as the secondary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92551).

    • ab92551 showing positive staining in Cervical carcinoma tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92551).

    • ab92551 showing positive staining in Normal tonsil squamous cells tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92551).

    • ab92551 showing negative staining in Glioma tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92551).

    • ab92551 showing negative staining in Breast carcinoma tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92551).

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92551).

    References

    ab239918 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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