Recombinant Anti-Cytokeratin 13 antibody [EPR3672] (ab133340)
![Western blot - Anti-Cytokeratin 13 antibody [EPR3672] (ab133340)](https://www.abcam.com/ps/products/133/ab133340/Images/ab133340-146886-anti-cytokeratin-13-antibody-epr3672-western-blot.gif "Western blot - Anti-Cytokeratin 13 antibody [EPR3672] (ab133340)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3672] to Cytokeratin 13
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Cytokeratin 13 antibody [EPR3672]
See all Cytokeratin 13 primary antibodies -
Description
Rabbit monoclonal [EPR3672] to Cytokeratin 13 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to residues in Human Cytokeratin 13. (UniProt: P13646).
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Positive control
- Human squamous cell cervical carcinoma tissue; Human transitional cell carcinoma of urinary bladder tissue; A431 and HACAT cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3672 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133340 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| Notes |
|---|
|
WB
1/2000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Tissue specificity
Expressed in some epidermal sweat gland ducts (at protein level) and in exocervix, esophagus and placenta. -
Involvement in disease
Defects in KRT13 are a cause of white sponge nevus of cannon (WSN) [MIM:193900]. WSN is a rare autosomal dominant disorder which predominantly affects non-cornified stratified squamous epithelia. Clinically, it is characterized by the presence of soft, white, and spongy plaques in the oral mucosa. The characteristic histopathologic features are epithelial thickening, parakeratosis, and vacuolization of the suprabasal layer of oral epithelial keratinocytes. Less frequently the mucous membranes of the nose, esophagus, genitalia and rectum are involved. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsO-glycosylated; glycans consist of single N-acetylglucosamine residues. - Information by UniProt
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Database links
- Entrez Gene: 3860 Human
- Omim: 148065 Human
- SwissProt: P13646 Human
- Unigene: 654550 Human
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Alternative names
- 47 kDa cytokeratin antibody
- CK-13 antibody
- CK13 antibody
see all
Images
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Western blot - Anti-Cytokeratin 13 antibody [EPR3672] (ab133340)All lanes : Anti-Cytokeratin 13 antibody [EPR3672] (ab133340) at 1/2000 dilution
Lane 1 : A431 cell lysate
Lane 2 : HACAT cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3672] (ab133340)](https://www.abcam.com/ps/products/133/ab133340/Images/ab133340-146885-anti-cytokeratin-13-antibody-epr3672-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3672] (ab133340)Immunohistochemical analysis of Cytokeratin 13 in formalin fixed, paraffin embedded Human squamous cell cervical carcinoma tissue stainied with ab133340 at a 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3672] (ab133340)](https://www.abcam.com/ps/products/133/ab133340/Images/ab133340-146884-anti-cytokeratin-13-antibody-epr3672-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3672] (ab133340)Immunohistochemical analysis of Cytokeratin 13 in formalin fixed, paraffin embedded Human transitional cell carcinoma of urinary bladder tissue stainied with ab133340 at a 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3672] (ab133340)](https://www.abcam.com/ps/products/133/ab133340/Images/ab133340-146883-anti-cytokeratin-13-antibody-epr3672-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3672] (ab133340)Immunohistochemical analysis of paraffin embedded Human Glioma tissue using ab133340 showing -ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3672] (ab133340)](https://www.abcam.com/ps/products/133/ab133340/Images/ab133340-146882-anti-cytokeratin-13-antibody-epr3672-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3672] (ab133340)Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab133340 showing -ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3672] (ab133340)](https://www.abcam.com/ps/products/133/ab133340/Images/ab133340-146881-anti-cytokeratin-13-antibody-epr3672-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3672] (ab133340)Immunohistochemical analysis of paraffin embedded normal Human tonsil squamous cells tissue using ab133340 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-Cytokeratin 13 antibody [EPR3672] (ab133340)](https://www.abcam.com/ps/products/133/ab133340/Images/ab133340-7-benefits-of-recombinant-antibodies.png)
Anti-Cytokeratin 13 antibody [EPR3672] (ab133340)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab133340 has been referenced in 1 publication.
- Liu S et al. Keratin 13 Is Enriched in Prostate Tubule-Initiating Cells and May Identify Primary Prostate Tumors that Metastasize to the Bone. PLoS One 11:e0163232 (2016). IHC ; Human . PubMed: 27711225