Recombinant
RabMAb

Recombinant Anti-Cytokeratin 13 antibody [EPR3672] (ab133340)

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Overview

  • Product name

    Anti-Cytokeratin 13 antibody [EPR3672]
    See all Cytokeratin 13 primary antibodies

  • Description

    Rabbit monoclonal [EPR3672] to Cytokeratin 13

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide corresponding to residues in Human Cytokeratin 13. (UniProt: P13646).

  • Positive control

    • Human squamous cell cervical carcinoma tissue; Human transitional cell carcinoma of urinary bladder tissue; A431 and HACAT cell lysates

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab133340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
ICC 1/50 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Tissue specificity

      Expressed in some epidermal sweat gland ducts (at protein level) and in exocervix, esophagus and placenta.

    • Involvement in disease

      Defects in KRT13 are a cause of white sponge nevus of cannon (WSN) [MIM:193900]. WSN is a rare autosomal dominant disorder which predominantly affects non-cornified stratified squamous epithelia. Clinically, it is characterized by the presence of soft, white, and spongy plaques in the oral mucosa. The characteristic histopathologic features are epithelial thickening, parakeratosis, and vacuolization of the suprabasal layer of oral epithelial keratinocytes. Less frequently the mucous membranes of the nose, esophagus, genitalia and rectum are involved.

    • Sequence similarities

      Belongs to the intermediate filament family.

    • Post-translational
      modifications

      O-glycosylated; glycans consist of single N-acetylglucosamine residues.
    • Information by UniProt

    • Database links

      • Alternative names

        • 47 kDa cytokeratin antibody
        • CK-13 antibody
        • CK13 antibody
        • Cytokeratin 13 antibody
        • Cytokeratin-13 antibody
        • K13 antibody
        • K1C13_HUMAN antibody
        • Ka13 antibody
        • Keratin 13 antibody
        • Keratin antibody
        • keratin type I cytoskeletal 13 antibody
        • Keratin-13 antibody
        • Krt-1.13 antibody
        • Krt1-13 antibody
        • KRT13 antibody
        • MGC161462 antibody
        • MGC3781 antibody
        • type I cytoskeletal 13 antibody
        • Type I keratin Ka13 antibody
        • WSN2 antibody
        see all

      Images

      • Western blot - Anti-Cytokeratin 13 antibody [EPR3672] (ab133340)
        Western blot - Anti-Cytokeratin 13 antibody [EPR3672] (ab133340)
        All lanes : Anti-Cytokeratin 13 antibody [EPR3672] (ab133340) at 1/2000 dilution

        Lane 1 : A431 cell lysate
        Lane 2 : HACAT cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 50 kDa
        Observed band size: 50 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3672] (ab133340)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3672] (ab133340)

        Immunohistochemical analysis of Cytokeratin 13 in formalin fixed, paraffin embedded Human squamous cell cervical carcinoma tissue stainied with ab133340 at a 1/100 dilution.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3672] (ab133340)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3672] (ab133340)

        Immunohistochemical analysis of Cytokeratin 13 in formalin fixed, paraffin embedded Human transitional cell carcinoma of urinary bladder tissue stainied with ab133340 at a 1/100 dilution.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3672] (ab133340)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3672] (ab133340)

        Immunohistochemical analysis of paraffin embedded Human Glioma tissue using ab133340 showing -ve staining.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3672] (ab133340)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3672] (ab133340)

        Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab133340 showing -ve staining.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3672] (ab133340)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3672] (ab133340)

        Immunohistochemical analysis of paraffin embedded normal Human tonsil squamous cells tissue using ab133340 showing +ve staining.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      References

      This product has been referenced in:

      • Liu S  et al. Keratin 13 Is Enriched in Prostate Tubule-Initiating Cells and May Identify Primary Prostate Tumors that Metastasize to the Bone. PLoS One 11:e0163232 (2016). IHC ; Human . Read more (PubMed: 27711225) »
      See 1 Publication for this product

      Publishing research using ab133340? Please let us know so that we can cite the reference in this datasheet.

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