Overview

  • Product name

    Anti-Cytokeratin 14 antibody [EPR1612]
    See all Cytokeratin 14 primary antibodies
  • Description

    Rabbit monoclonal [EPR1612] to Cytokeratin 14
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-P, ICCmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Cytokeratin 14. The exact sequence is proprietary.

  • Positive control

    • WB: Human skin and A431 cell lysates IHC-P: Human skin, tonsil and squamous cell cervical carcinoma tissues
  • General notes

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab108417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
WB 1/10000 - 1/50000. Predicted molecular weight: 52 kDa.
IHC-P 1/250 - 1/500. Antigen retrieval is recommended.
ICC 1/250 - 1/500.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
    • Tissue specificity

      Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen.
    • Involvement in disease

      Defects in KRT14 are a cause of epidermolysis bullosa simplex Dowling-Meara type (DM-EBS) [MIM:131760]. DM-EBS is a severe form of intraepidermal epidermolysis bullosa characterized by generalized herpetiform blistering, milia formation, dystrophic nails, and mucous membrane involvement.
      Defects in KRT14 are a cause of epidermolysis bullosa simplex Weber-Cockayne type (WC-EBS) [MIM:131800]. WC-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering limited to palmar and plantar areas of the skin.
      Defects in KRT14 are a cause of epidermolysis bullosa simplex Koebner type (K-EBS) [MIM:131900]. K-EBS is a form of intraepidermal epidermolysis bullosa characterized by generalized skin blistering. The phenotype is not fundamentally distinct from the Dowling-Meara type, although it is less severe.
      Defects in KRT14 are the cause of epidermolysis bullosa simplex autosomal recessive (AREBS) [MIM:601001]. AREBS is an intraepidermal epidermolysis bullosa characterized by localized blistering on the dorsal, lateral and plantar surfaces of the feet.
      Defects in KRT14 are the cause of Naegeli-Franceschetti-Jadassohn syndrome (NFJS) [MIM:161000]; also known as Naegeli syndrome. NFJS is a rare autosomal dominant form of ectodermal dysplasia. The cardinal features are absence of dermatoglyphics (fingerprints), reticular cutaneous hyperpigmentation (starting at about the age of 2 years without a preceding inflammatory stage), palmoplantar keratoderma, hypohidrosis with diminished sweat gland function and discomfort provoked by heat, nail dystrophy, and tooth enamel defects.
      Defects in KRT14 are the cause of dermatopathia pigmentosa reticularis (DPR) [MIM:125595]. DPR is a rare ectodermal dysplasia characterized by lifelong persistent reticulate hyperpigmentation, noncicatricial alopecia, and nail dystrophy.
    • Sequence similarities

      Belongs to the intermediate filament family.
    • Cellular localization

      Cytoplasm. Nucleus. Expressed in both as a filamentous pattern.
    • Information by UniProt
    • Database links

    • Alternative names

      • CK 14 antibody
      • CK-14 antibody
      • ck14 antibody
      • Cytokeratin 14 antibody
      • Cytokeratin-14 antibody
      • Cytokeratin14 antibody
      • Dowling Meara antibody
      • EBS3 antibody
      • EBS4 antibody
      • Epidermolysis bullosa simplex antibody
      • K14 antibody
      • K1C14_HUMAN antibody
      • Keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner) antibody
      • Keratin 14 antibody
      • Keratin antibody
      • Keratin type I cytoskeletal 14 antibody
      • Keratin, type I cytoskeletal 14 antibody
      • Keratin-14 antibody
      • Keratin14 antibody
      • Koebner antibody
      • Krt 14 antibody
      • Krt14 antibody
      • NFJ antibody
      • OTTHUMP00000164624 antibody
      • type I cytoskeletal 14 antibody
      see all

    Images

    • ab108417, at a 1/250 dilution, staining Cytokeratin 14 in paraffin embedded Human squamous cell cervical carcinoma tissue by Immunohistochemistry.
    • Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labeling Cytokeratin 14 with purified ab108417 at 1:250 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077)(1:2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • Anti-Cytokeratin 14 antibody [EPR1612] (ab108417) at 1/10000 dilution + Human skin lysate at 10 µg

      Predicted band size: 52 kDa

    • Anti-Cytokeratin 14 antibody [EPR1612] (ab108417) at 1/10000 dilution + A431 cell lysate at 10 µg

      Predicted band size: 52 kDa

    • ab108417, at a 1/250 dilution, staining Cytokeratin 14 in paraffin embedded Human skin tissue by Immunohistochemistry.
    • ab108417 showing positive staining in Normal prostate basal epithelium tissue.

    • ab108417, at a 1/250 dilution, staining Cytokeratin 14 in paraffin embedded Human tonsil tissue by Immunohistochemistry.
    • ab108417 showing negative staining in Normal kidney tissue.

    • ab108417 showing negative staining in Breast carcinoma tissue.

    • ab108417 showing negative staining in Colonic adenocarcinoma tissue.

    • ab108417 showing positive staining in Normal skin tissue.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab108417 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Answer

    Thank you for contacting us.

    Your credit note ID is x.

    I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department.

    Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

    The credit note ID is for your reference only and does not automatically guarantee the credit.

    I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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    Answer

    Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this Cytokeratin 14antibody.

    The details you have kindly provided will provide us with vital information for our monitoring of product quality.

    I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. I am sorry there are no further tips to provide on this occasion to help improve the results, as your protocol seems absolutely fine to me. I have attached our IHC protocol for your reference, just in case you spot something I missed.

    Reviewing the details of this case, I can suggest you have regrettably received a bad vial. I apologise for the inconvenience and am pleased to offer you a free of charge replacement (with a primary antibody of your choice) or a credit note in compensation.

    In order to arrange this, could you please confirm the following:

    1) Does'have not had any positive results' mean no staining at all, non-specific background staining or an incorrect staining pattern?

    2)Could you confirm the order number x?
    Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

    Read More

    Answer

    Gracias por contactarnos.

    Como bien dices, tenemos varios anticuerpos contra los marcadores especificados, sin embargo, que se hayan testado y validado (y por tanto, estén cubiertos por la garantía Abpromise) en inmunocitoquímica son muchos menos.

    La citoqueratina 14 es en efecto un buen marcador de células miopiteliales en glándula mamaria, y aunque ninguno de los tres anticuerpos testados en ICC se haya probado en estas células, todos ellos deberían funcionar.

    Los enlaces a las datasheets son:

    https://www.abcam.com/Cytokeratin-14-antibody-RCK107-ab9220.html

    https://www.abcam.com/Cytokeratin-14-antibody-EPR1612-ab108417.html

    https://www.abcam.com/Cytokeratin-14-antibody-SPK-14-ab9384.html

    Cualquiera de ellos es una buena opción. El primero tiene una imagen de células HepG2 teñidas con el anticuerpo. Los rabbit monoclonals (ab108414) suelen ser muy limpios y específicos. De todas maneras todos ellos están garantizados en ICC, por lo que en caso de ni funcionar correctamente se os mandaría un reemplazo (del mismo o diferente anticuerpo) gratuito o un reembolso del importe.

    Otros marcadores de celular mioepiteliales son la citoqueratina 17, p-cadherin o, Vimentin. Para este último tenemos varios anticuerpos. Para vuestro caso concreto ab8978 puede ser una buena opción, y como podéis observar en la pestaña “Abreviews” otros usuarios lo han utilizado obteniendo excelentes resultados:

    https://www.abcam.com/Vimentin-antibody-RV202-Neural-Stem-Cell-Marker-ab8978.html

    Contra la citoquina 19 las alternativas son mayores, y aunque se hayan usado en líneas celulares muy distintas, podéis ver los Reviews de otros usuarios, que vuelven a ser muy positivos en ICC.

    https://www.abcam.com/Cytokeratin-19-antibody-ab15463.html

    https://www.abcam.com/Cytokeratin-19-antibody-RCK108-ab9221.html

    En cualquier caso como mencioné antes, todos tienen una garantía de 6 meses, y en el caso de no funcionar correctamente se os mandaría un reemplazo o rembolso.

    Actualmente tenemos una promoción por la que por la compra de 3 productos se os regala uno adicional (un 4x3). Si necesitáis más información sobre alguno de estos productos, un presupuesto o alguna aclaración, por favor, no dudes en contactarme de nuevo.

    Read More

    Answer

    Thank you very much for contacting us. Following your request, I have searched our catalog for all antibodies that match the mentioned criteria, i.e. that recognise human targets and that do not react with mouse. Please find attached a list of these antibodies. Among these are a number of anti-cytokeratin antibodies (e.g. ab76539, ab76318, and ab108417) which we can guarantee that they do not react with the mouse orthologs, as it has been tested specifically. This is also applicable to other targets, such as MRP1 (ab3369). However, there are a few antibodies on this list which state on the datasheet that they do not react with mouse, but this information is based on sequence similarities only and has not been tested. I would be pleased to let you know though if we can guarantee that the antibody you have chosen does not cross-react with mouse. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

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