Question (42706) | Anti-Cytokeratin 14 antibody [EPR1612] (ab108417)

Go to datasheet (ab108417)

Question

Hello,

I am writing in regards to the Anti-Cytokeratin 14 (ab108417) we recently purchased from you. I have made several attempts at staining with this antibody and have not had any positive results. Below is what I have tried, Is there something I am missing or is it possible that the antibody is not any good? The control tissue was a sample of Human skin.

Run #1

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Run #2

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Thank you for your time regarding this issue.

Answer

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this Cytokeratin 14antibody.

The details you have kindly provided will provide us with vital information for our monitoring of product quality.

I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. I am sorry there are no further tips to provide on this occasion to help improve the results, as your protocol seems absolutely fine to me. I have attached our IHC protocol for your reference, just in case you spot something I missed.

Reviewing the details of this case, I can suggest you have regrettably received a bad vial. I apologise for the inconvenience and am pleased to offer you a free of charge replacement (with a primary antibody of your choice) or a credit note in compensation.

In order to arrange this, could you please confirm the following:

1) Does'have not had any positive results' mean no staining at all, non-specific background staining or an incorrect staining pattern?

2)Could you confirm the order number x?
Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

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