Overview

  • Product name

    Anti-Cytokeratin 14 antibody [EPR17336]
    See all Cytokeratin 14 primary antibodies
  • Description

    Rabbit monoclonal [EPR17336] to Cytokeratin 14
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Mouse Cytokeratin aa 450 to the C-terminus. The exact sequence is proprietary.
    Database link: Q61781

  • Positive control

    • WB: Mouse and rat skin lysate. Human fetal skin lysate. IHC-P: Human squamous cell carcinoma of cervix tissue. Mouse skin tissue. ICC/IF: HaCaT cells. A431 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA, 49% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR17336
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab197893 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/160.
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/50000. Predicted molecular weight: 66 kDa.

Target

  • Function

    The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
  • Tissue specificity

    Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen.
  • Involvement in disease

    Defects in KRT14 are a cause of epidermolysis bullosa simplex Dowling-Meara type (DM-EBS) [MIM:131760]. DM-EBS is a severe form of intraepidermal epidermolysis bullosa characterized by generalized herpetiform blistering, milia formation, dystrophic nails, and mucous membrane involvement.
    Defects in KRT14 are a cause of epidermolysis bullosa simplex Weber-Cockayne type (WC-EBS) [MIM:131800]. WC-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering limited to palmar and plantar areas of the skin.
    Defects in KRT14 are a cause of epidermolysis bullosa simplex Koebner type (K-EBS) [MIM:131900]. K-EBS is a form of intraepidermal epidermolysis bullosa characterized by generalized skin blistering. The phenotype is not fundamentally distinct from the Dowling-Meara type, although it is less severe.
    Defects in KRT14 are the cause of epidermolysis bullosa simplex autosomal recessive (AREBS) [MIM:601001]. AREBS is an intraepidermal epidermolysis bullosa characterized by localized blistering on the dorsal, lateral and plantar surfaces of the feet.
    Defects in KRT14 are the cause of Naegeli-Franceschetti-Jadassohn syndrome (NFJS) [MIM:161000]; also known as Naegeli syndrome. NFJS is a rare autosomal dominant form of ectodermal dysplasia. The cardinal features are absence of dermatoglyphics (fingerprints), reticular cutaneous hyperpigmentation (starting at about the age of 2 years without a preceding inflammatory stage), palmoplantar keratoderma, hypohidrosis with diminished sweat gland function and discomfort provoked by heat, nail dystrophy, and tooth enamel defects.
    Defects in KRT14 are the cause of dermatopathia pigmentosa reticularis (DPR) [MIM:125595]. DPR is a rare ectodermal dysplasia characterized by lifelong persistent reticulate hyperpigmentation, noncicatricial alopecia, and nail dystrophy.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Cellular localization

    Cytoplasm. Nucleus. Expressed in both as a filamentous pattern.
  • Information by UniProt
  • Database links

  • Alternative names

    • CK 14 antibody
    • CK-14 antibody
    • ck14 antibody
    • Cytokeratin 14 antibody
    • Cytokeratin-14 antibody
    • Cytokeratin14 antibody
    • Dowling Meara antibody
    • EBS3 antibody
    • EBS4 antibody
    • Epidermolysis bullosa simplex antibody
    • K14 antibody
    • K1C14_HUMAN antibody
    • Keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner) antibody
    • Keratin 14 antibody
    • Keratin antibody
    • Keratin type I cytoskeletal 14 antibody
    • Keratin, type I cytoskeletal 14 antibody
    • Keratin-14 antibody
    • Keratin14 antibody
    • Koebner antibody
    • Krt 14 antibody
    • Krt14 antibody
    • NFJ antibody
    • OTTHUMP00000164624 antibody
    • type I cytoskeletal 14 antibody
    see all

Images

  • All lanes : Anti-Cytokeratin 14 antibody [EPR17336] (ab197893) at 1/50000 dilution

    Lane 1 : Mouse skin lysate at 20 µg
    Lane 2 : Rat skin lysate at 20 µg
    Lane 3 : Rat brain lysate at 10 µg
    Lane 4 : Rat heart lysate at 10 µg
    Lane 5 : Rat kidney lysate at 10 µg
    Lane 6 : Rat spleen lysate at 10 µg
    Lane 7 : C6(Rat glial tumor cells) whole cell lysate at 10 µg
    Lane 8 : Raw264.7(Mouse macrophage cells transformed with Abelson murine leukemia virus ) whole cell lysate at 10 µg
    Lane 9 : PC-12(Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg
    Lane 10 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 66 kDa
    Observed band size: 53 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    5% NFDM/TBST: Blocking and diluting buffer.

    The expression of Cytokeratin 14 is basal cells of epidermis and other stratified epithelia. Lanes 3-10 represent Cytokeratin 14 negative tissues and cell lines.

  • Anti-Cytokeratin 14 antibody [EPR17336] (ab197893) at 1/50000 dilution + human fetal skin lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 66 kDa
    Observed band size: 53 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    5% NFDM/TBST: Blocking and diluting buffer.

  • Immunohistochemical analysis of paraffin-embedded Humansquamous cell carcinoma of cervix tissue labeling Cytokeratin 14 using ab197893 at 1/500 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab197893 and secondary antibody only.
    Note: Cytoplasm staining on human squamous cell carcinoma of cervix tissue was observed.

  • Immunohistochemical analysis of paraffin-embedded Mouse skin tissue labeling Cytokeratin 14 using ab197893 at 1/500 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab197893 and secondary antibody only.
    Note: Cytoplasm staining on mouse skin tissue was observed.

References

ab197893 has not yet been referenced specifically in any publications.

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