Anti-Cytokeratin 14 antibody [LL002] (ab7800)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody [LL002] (ab7800)](https://www.abcam.com/ps/products/7/ab7800/Images/ab7800-331584-AP40398481ugmlhuskin.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody [LL002] (ab7800)")
Key features and details
- Mouse monoclonal [LL002] to Cytokeratin 14
- Suitable for: WB, IHC-P, ICC
- Knockout validated
- Reacts with: Human
- Isotype: IgG3
Related conjugates and formulations
Overview
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Product name
Anti-Cytokeratin 14 antibody [LL002]
See all Cytokeratin 14 primary antibodies -
Description
Mouse monoclonal [LL002] to Cytokeratin 14 -
Host species
Mouse -
Specificity
This antibody labels the basal layer of stratifying squamous and non-squamous epithelia. The staining pattern iscytoplasmic. It recognizes basal cell carcinomas and squamous cell carcinomas.
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Tested applications
Suitable for: WB, IHC-P, ICCmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to Human Cytokeratin 14 (C terminal).
Database link: P02533 -
Positive control
- IHC-P: Human normal skin tissue sections. ICC: A431 cells. WB: A431 whole cell lysate. Human skin whole tissue lysate
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
Protein A purified -
Primary antibody notes
This antibody labels the basal layer of stratifying squamous and non-squamous epithelia. The staining pattern iscytoplasmic. It recognizes basal cell carcinomas and squamous cell carcinomas. -
Clonality
Monoclonal -
Clone number
LL002 -
Isotype
IgG3 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7800 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (4) |
Use a concentration of 1 µg/ml.
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| IHC-P | (14) |
Use a concentration of 0.1 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC | (1) |
Use a concentration of 0.1 - 1 µg/ml.
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| Notes |
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WB
Use a concentration of 1 µg/ml. |
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IHC-P
Use a concentration of 0.1 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC
Use a concentration of 0.1 - 1 µg/ml. |
Target
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Function
The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro. -
Tissue specificity
Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen. -
Involvement in disease
Defects in KRT14 are a cause of epidermolysis bullosa simplex Dowling-Meara type (DM-EBS) [MIM:131760]. DM-EBS is a severe form of intraepidermal epidermolysis bullosa characterized by generalized herpetiform blistering, milia formation, dystrophic nails, and mucous membrane involvement.
Defects in KRT14 are a cause of epidermolysis bullosa simplex Weber-Cockayne type (WC-EBS) [MIM:131800]. WC-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering limited to palmar and plantar areas of the skin.
Defects in KRT14 are a cause of epidermolysis bullosa simplex Koebner type (K-EBS) [MIM:131900]. K-EBS is a form of intraepidermal epidermolysis bullosa characterized by generalized skin blistering. The phenotype is not fundamentally distinct from the Dowling-Meara type, although it is less severe.
Defects in KRT14 are the cause of epidermolysis bullosa simplex autosomal recessive (AREBS) [MIM:601001]. AREBS is an intraepidermal epidermolysis bullosa characterized by localized blistering on the dorsal, lateral and plantar surfaces of the feet.
Defects in KRT14 are the cause of Naegeli-Franceschetti-Jadassohn syndrome (NFJS) [MIM:161000]; also known as Naegeli syndrome. NFJS is a rare autosomal dominant form of ectodermal dysplasia. The cardinal features are absence of dermatoglyphics (fingerprints), reticular cutaneous hyperpigmentation (starting at about the age of 2 years without a preceding inflammatory stage), palmoplantar keratoderma, hypohidrosis with diminished sweat gland function and discomfort provoked by heat, nail dystrophy, and tooth enamel defects.
Defects in KRT14 are the cause of dermatopathia pigmentosa reticularis (DPR) [MIM:125595]. DPR is a rare ectodermal dysplasia characterized by lifelong persistent reticulate hyperpigmentation, noncicatricial alopecia, and nail dystrophy. -
Sequence similarities
Belongs to the intermediate filament family. -
Cellular localization
Cytoplasm. Nucleus. Expressed in both as a filamentous pattern. - Information by UniProt
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Database links
- Entrez Gene: 3861 Human
- Entrez Gene: 16664 Mouse
- Entrez Gene: 287701 Rat
- Omim: 148066 Human
- SwissProt: P02533 Human
- SwissProt: Q61781 Mouse
- SwissProt: Q6IFV1 Rat
- Unigene: 654380 Human
see all -
Alternative names
- CK 14 antibody
- CK-14 antibody
- ck14 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody [LL002] (ab7800)
IHC image of Cytokeratin 14 staining in a section of formalin-fixed paraffin-embedded normal human skin* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab7800, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Immunocytochemistry - Anti-Cytokeratin 14 antibody [LL002] (ab7800)](https://www.abcam.com/ps/products/7/ab7800/Images/ab7800-15-anti-KRT14-antibody-LL002-immunocytochemistry-immunofluorescence-A431-human.jpg)
Immunocytochemistry - Anti-Cytokeratin 14 antibody [LL002] (ab7800)ab7800 staining KRT14 in wild-type A431 cells (top panel) and KRT14 knockout A431 cells (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab7800 at 1μg/ml concentration and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8). -
![Western blot - Anti-Cytokeratin 14 antibody [LL002] (ab7800)](https://www.abcam.com/ps/products/7/ab7800/Images/ab7800-343118-Ab7800AP4039484westernblotannotated.jpg)
Western blot - Anti-Cytokeratin 14 antibody [LL002] (ab7800)All lanes :
Lane 1 : A431 whole cell lysate
Lane 2 : Human skin whole tissue lysate
Lane 3 : SH-SY5Y whole cell lysate (negative control)
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Observed band size: 55 kDa why is the actual band size different from the predicted?
Additional bands at: 70 kDa (possible cross reactivity)This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before ab7800 and ab181602 (Rabbit anti GAPDH), were incubated overnight at 4°C at a 1ug/ml concentration and 1/20000 dilution respectively. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry - Anti-Cytokeratin 14 antibody [LL002] (ab7800)](https://www.abcam.com/ps/products/7/ab7800/Images/ab7800-331586-AP403984801ugmlA431mjpg.jpg)
Immunocytochemistry - Anti-Cytokeratin 14 antibody [LL002] (ab7800)ab7800 staining Cytokeratin 14 in A431 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab7800 at 0.1ugml then detected with an Alexa Fluor® 488 goat anti-mouse secondary antibody (ab150117) at a 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue), and ab202272, Rabbit monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody [LL002] (ab7800)](https://www.abcam.com/ps/products/7/ab7800/Images/ab7800-13275-anti-cytokeratin-14-antibody-ll002-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody [LL002] (ab7800)This image is courtesy of an anonymous Abreviewab7800 staining Cytokeratin 14 in Human normal skin tissue sections by IHC-P (Formaldehyde-fixed, Paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with 10% Serum for 30 minutes at 21°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). The sample was incubated with primary antibody (1/100 in PBS + 0.5% Tween-20 + 0.5% BSA)) at 21°C for 30 minutes. An undiluted HRP-conjugated goat polyclonal to mouse IgG was used as secondary antibody. -
![Western blot - Anti-Cytokeratin 14 antibody [LL002] (ab7800)](https://www.abcam.com/ps/products/7/ab7800/Images/ab7800-13274-anti-cytokeratin-14-antibody-ll002-western-blot.jpg)
Western blot - Anti-Cytokeratin 14 antibody [LL002] (ab7800)This image is courtesy of an anonymous AbreviewAnti-Cytokeratin 14 antibody [LL002] (ab7800) at 2 µg/ml + Human HaCaT whole cell lysate at 30 µg
Secondary
Goat Anti-mouse IgG Polyclonal at 1/20000 dilution
Developed using the ECL technique.
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
Blocking Step: 5% Milk for 12 hours at 4°C
Gel Running Conditions: 15%,6V,50min; Reduced; Denaturing
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (161)
ab7800 has been referenced in 161 publications.
- Wang Z et al. Overexpression of Fgf8 in the epidermis inhibits hair follicle development. Exp Dermatol 30:494-502 (2021). PubMed: 33141445
- Panciera T et al. Reprogramming normal cells into tumour precursors requires ECM stiffness and oncogene-mediated changes of cell mechanical properties. Nat Mater 19:797-806 (2020). PubMed: 32066931
- Tang H et al. MicroRNA-200b/c-3p regulate epithelial plasticity and inhibit cutaneous wound healing by modulating TGF-ß-mediated RAC1 signaling. Cell Death Dis 11:931 (2020). PubMed: 33122632
- Makino T et al. Trichohyalin-like 1 protein plays a crucial role in proliferation and anti-apoptosis of normal human keratinocytes and squamous cell carcinoma cells. Cell Death Discov 6:109 (2020). PubMed: 33133644
- Hu L et al. Human esophageal myofibroblasts increase squamous epithelial thickness via paracrine mechanisms in an in vitro model of gastroesophageal reflux disease. PLoS One 15:e0238852 (2020). PubMed: 32925965