Product nameAnti-Cytokeratin 14 antibody [LL002]
See all Cytokeratin 14 primary antibodies
DescriptionMouse monoclonal [LL002] to Cytokeratin 14
Tested applicationsSuitable for: WB, Flow Cyt, ICC/IF, IHC-P, IHC-Frmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human Cytokeratin 14 (C terminal).
Database link: P02533
This antibody labels the basal layer of stratifying squamous and non-squamous epithelia. The staining pattern iscytoplasmic. It recognizes basal cell carcinomas and squamous cell carcinomas.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
Prepared in 10mM PBS
PurityTissue culture supernatant
Primary antibody notesThis antibody labels the basal layer of stratifying squamous and non-squamous epithelia. The staining pattern iscytoplasmic. It recognizes basal cell carcinomas and squamous cell carcinomas.
Our Abpromise guarantee covers the use of ab7800 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
0.5-1ug/million cells in 0.1ml
ab91537 - Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use a concentration of 0.5 - 1 µg/ml.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Incubate for 30 min at RT
|IHC-Fr||1/20 - 1/300. PubMed: 23769181|
FunctionThe nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
Tissue specificityDetected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen.
Involvement in diseaseDefects in KRT14 are a cause of epidermolysis bullosa simplex Dowling-Meara type (DM-EBS) [MIM:131760]. DM-EBS is a severe form of intraepidermal epidermolysis bullosa characterized by generalized herpetiform blistering, milia formation, dystrophic nails, and mucous membrane involvement.
Defects in KRT14 are a cause of epidermolysis bullosa simplex Weber-Cockayne type (WC-EBS) [MIM:131800]. WC-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering limited to palmar and plantar areas of the skin.
Defects in KRT14 are a cause of epidermolysis bullosa simplex Koebner type (K-EBS) [MIM:131900]. K-EBS is a form of intraepidermal epidermolysis bullosa characterized by generalized skin blistering. The phenotype is not fundamentally distinct from the Dowling-Meara type, although it is less severe.
Defects in KRT14 are the cause of epidermolysis bullosa simplex autosomal recessive (AREBS) [MIM:601001]. AREBS is an intraepidermal epidermolysis bullosa characterized by localized blistering on the dorsal, lateral and plantar surfaces of the feet.
Defects in KRT14 are the cause of Naegeli-Franceschetti-Jadassohn syndrome (NFJS) [MIM:161000]; also known as Naegeli syndrome. NFJS is a rare autosomal dominant form of ectodermal dysplasia. The cardinal features are absence of dermatoglyphics (fingerprints), reticular cutaneous hyperpigmentation (starting at about the age of 2 years without a preceding inflammatory stage), palmoplantar keratoderma, hypohidrosis with diminished sweat gland function and discomfort provoked by heat, nail dystrophy, and tooth enamel defects.
Defects in KRT14 are the cause of dermatopathia pigmentosa reticularis (DPR) [MIM:125595]. DPR is a rare ectodermal dysplasia characterized by lifelong persistent reticulate hyperpigmentation, noncicatricial alopecia, and nail dystrophy.
Sequence similaritiesBelongs to the intermediate filament family.
Cellular localizationCytoplasm. Nucleus. Expressed in both as a filamentous pattern.
- Information by UniProt
- CK 14 antibody
- CK-14 antibody
- ck14 antibody
ab7800 staining Cytokeratin 14 in Human normal skin tissue sections by IHC-P (Formaldehyde-fixed, Paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with 10% Serum for 30 minutes at 21°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). The sample was incubated with primary antibody (1/100 in PBS + 0.5% Tween-20 + 0.5% BSA)) at 21°C for 30 minutes. An undiluted HRP-conjugated goat polyclonal to mouse IgG was used as secondary antibody.
ab7800 staining Cytokeratin 14 in Human Prostate Carcinoma tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
ab7800 was used to stain mouse prostate.
Anti-Cytokeratin 14 antibody [LL002] (ab7800) at 2 µg/ml + Human HaCaT whole cell lysate at 30 µg
Goat Anti-mouse IgG Polyclonal at 1/20000 dilution
Developed using the ECL technique.
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
Blocking Step: 5% Milk for 12 hours at 4°C
Gel Running Conditions: 15%,6V,50min; Reduced; Denaturing
ab7800 staining Cytokeratin 14 in Human tonsil tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Immunofluorescence analysis of mouse mammary epithelial cells, staining Cytokeratin 14 (red) with ab7800.
Immunohistochemical analysis of mouse mammary duct tissue, staining Cytokeratin 14 (red) with ab7800.
Antigen retrieval was carried out on paraffin-embedded sections by boiling in citrate buffer (pH 6) for 18 minutes in a microwave. Sections were then blocked for 1.5 hours in blocking reagents, before incubating with primary antibody (0.26 µg/ml) overnight at 4°C. An AlexaFluor®555-conjugated goat anti-mouse IgG was used as the secondary antibody
This product has been referenced in:
- Rogerson C & Gissen P VPS33B and VIPAR are essential for epidermal lamellar body biogenesis and function. Biochim Biophys Acta 1864:1609-1621 (2018). Read more (PubMed: 29409756) »
- Vielmuth F et al. Keratins Regulate p38MAPK-Dependent Desmoglein Binding Properties in Pemphigus. Front Immunol 9:528 (2018). ICC/IF ; Mouse . Read more (PubMed: 29616033) »