Anti-Cytokeratin 14 antibody [RCK107] (ab9220)
- Datasheet
- References (17)
- Protocols
Overview
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Product nameAnti-Cytokeratin 14 antibody [RCK107]
See all Cytokeratin 14 primary antibodies -
DescriptionMouse monoclonal [RCK107] to Cytokeratin 14
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Host speciesMouse
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SpecificityThis antibody reacts exclusively with cytokeratin 14 which is present in basal cell compartments of stratified and combined epithelia.
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Tested applicationsSuitable for: Dot blot, ICC, IHC-Fr, WB, ICC/IF, IHC-P, Flow Cytmore details
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Species reactivityReacts with: Rat, Dog, Human, Pig
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Immunogen
Cytoskeletal preparation of TR146 epithelial cells.
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Positive control
- cytoskeletal preparation of TR146 epithelial cells, skin tissue lysate (Human)
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General notes
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
PurityProtein A purified
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Primary antibody notesCytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
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ClonalityMonoclonal
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Clone numberRCK107
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MyelomaSp2/0-Ag14
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab9220 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Dot blot | 1/100 - 1/1000. | |
| ICC | Use at an assay dependent concentration. | |
| IHC-Fr | 1/100 - 1/200. | |
| WB | 1/100 - 1/1000. | |
| ICC/IF | Use a concentration of 1 µg/ml. | |
| IHC-P | 1/50 - 1/7000. PubMed: 19812262 Heat retrieval (PubMed 19812262) and Proteinase K () |
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| Flow Cyt | 1/100 - 1/200. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Target
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FunctionThe nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
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Tissue specificityDetected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen.
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Involvement in diseaseDefects in KRT14 are a cause of epidermolysis bullosa simplex Dowling-Meara type (DM-EBS) [MIM:131760]. DM-EBS is a severe form of intraepidermal epidermolysis bullosa characterized by generalized herpetiform blistering, milia formation, dystrophic nails, and mucous membrane involvement.
Defects in KRT14 are a cause of epidermolysis bullosa simplex Weber-Cockayne type (WC-EBS) [MIM:131800]. WC-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering limited to palmar and plantar areas of the skin.
Defects in KRT14 are a cause of epidermolysis bullosa simplex Koebner type (K-EBS) [MIM:131900]. K-EBS is a form of intraepidermal epidermolysis bullosa characterized by generalized skin blistering. The phenotype is not fundamentally distinct from the Dowling-Meara type, although it is less severe.
Defects in KRT14 are the cause of epidermolysis bullosa simplex autosomal recessive (AREBS) [MIM:601001]. AREBS is an intraepidermal epidermolysis bullosa characterized by localized blistering on the dorsal, lateral and plantar surfaces of the feet.
Defects in KRT14 are the cause of Naegeli-Franceschetti-Jadassohn syndrome (NFJS) [MIM:161000]; also known as Naegeli syndrome. NFJS is a rare autosomal dominant form of ectodermal dysplasia. The cardinal features are absence of dermatoglyphics (fingerprints), reticular cutaneous hyperpigmentation (starting at about the age of 2 years without a preceding inflammatory stage), palmoplantar keratoderma, hypohidrosis with diminished sweat gland function and discomfort provoked by heat, nail dystrophy, and tooth enamel defects.
Defects in KRT14 are the cause of dermatopathia pigmentosa reticularis (DPR) [MIM:125595]. DPR is a rare ectodermal dysplasia characterized by lifelong persistent reticulate hyperpigmentation, noncicatricial alopecia, and nail dystrophy. -
Sequence similaritiesBelongs to the intermediate filament family.
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Cellular localizationCytoplasm. Nucleus. Expressed in both as a filamentous pattern.
- Information by UniProt
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Database links
- Entrez Gene: 3861 Human
- Entrez Gene: 287701 Rat
- Omim: 148066 Human
- SwissProt: P02533 Human
- SwissProt: Q6IFV1 Rat
- Unigene: 654380 Human
- Unigene: 145931 Rat
- Unigene: 153972 Rat
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Alternative names
- CK 14 antibody
- CK-14 antibody
- ck14 antibody
see all
Images
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![Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 14 antibody [RCK107] (ab9220)](https://www.abcam.com/ps/products/9/ab9220/Images/ab9220-252452-ab9220IHCFrozen.jpg)
Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 14 antibody [RCK107] (ab9220)Immunohistochemical analysis of swine skin tissue labeling Cytokeratin 14 with ab9220. Showing staining of basal cells.
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![Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 14 antibody [RCK107] (ab9220)](https://www.abcam.com/ps/products/9/ab9220/Images/Cytokeratin-14-Primary-antibodies-ab9220-1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 14 antibody [RCK107] (ab9220)ICC/IF image of ab9220 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9220, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
![Western blot - Anti-Cytokeratin 14 antibody [RCK107] (ab9220)](https://www.abcam.com/ps/products/9/ab9220/Images/Cytokeratin-14-Primary-antibodies-ab9220-6.jpg)
Western blot - Anti-Cytokeratin 14 antibody [RCK107] (ab9220)Anti-Cytokeratin 14 antibody [RCK107] (ab9220) at 4 µg/ml + Human skin tissue lysate - total protein (ab30166) at 20 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 58 kDa why is the actual band size different from the predicted?
Additional bands at: 72 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes -
![Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 14 antibody [RCK107] (ab9220)](https://www.abcam.com/ps/products/9/ab9220/Images/ab9220-305220-28.jpg)
Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 14 antibody [RCK107] (ab9220)Immunohistochemical analysis of dog skin epidermis tissue labelling Cytokeratin 14 using ab9220. Staining of basal keratinocyte layer is observed.
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![Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 14 antibody [RCK107] (ab9220)](https://www.abcam.com/ps/products/9/ab9220/Images/ab9220-305221-29.jpg)
Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 14 antibody [RCK107] (ab9220)Immunohistochemical analysis of dog skin appendages labelling Cytokeratin 14 with ab9220.
Protocols
References
This product has been referenced in:
- Zubeldia-Plazaola A et al. Glucocorticoids promote transition of ductal carcinoma in situ to invasive ductal carcinoma by inducing myoepithelial cell apoptosis. Breast Cancer Res 20:65 (2018). Read more (PubMed: 29973218) »
- Roth M et al. Broncho Vaxom (OM-85) modulates rhinovirus docking proteins on human airway epithelial cells via Erk1/2 mitogen activated protein kinase and cAMP. PLoS One 12:e0188010 (2017). Read more (PubMed: 29182620) »
