Overview

  • Product name

    Anti-Cytokeratin 15 antibody
    See all Cytokeratin 15 primary antibodies
  • Description

    Rabbit polyclonal to Cytokeratin 15
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Cytokeratin 15 (internal sequence). The exact sequence is proprietary.
    Database link: P19012

  • Positive control

    • WB: NTERA-2 cl.D1 [NT2/D1] whole cell lysate. IHC-P: Cal27 xenograft tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 49 kDa.

Target

  • Tissue specificity

    Expressed in a discontinuous manner in the basal cell layer of adult skin epidermis, but continuously in the basal layer of fetal skin epidermis and nail. Also expressed in the outer root sheath above the hair bulb in hair follicle (at protein level). Expressed homogeneously in all cell layers of the esophagus and exocervix, but detected in the basal cell layer only of oral mucosa, skin and in the basal plus the next two layers of the suprabasal epithelium of the palate.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Information by UniProt
  • Database links

  • Alternative names

    • AI528832 antibody
    • CK 15 antibody
    • CK-15 antibody
    • CK15 antibody
    • Cytokeratin-15 antibody
    • Cytokeratin15 antibody
    • K15 antibody
    • K1C15_HUMAN antibody
    • K1CO antibody
    • Ka15 antibody
    • Keratin 15 antibody
    • Keratin 15 basic antibody
    • Keratin 15 beta antibody
    • Keratin antibody
    • Keratin complex 1 acidic gene 15 antibody
    • Keratin type I cytoskeletal 15 antibody
    • Keratin-15 antibody
    • Keratin15 antibody
    • KRT 15 antibody
    • Krt1-15 antibody
    • KRT15 antibody
    • KRTB antibody
    • KRTL15 antibody
    • Type I cytoskeletal 15 antibody
    • Type I keratin Ka15 antibody
    see all

Images

  • Anti-Cytokeratin 15 antibody (ab227265) at 1/1000 dilution + NTERA-2 cl.D1 [NT2/D1] (human malignant pluripotent embryonic carcinoma cell line) whole cell lysate at 30 µg

    Predicted band size: 49 kDa



    10% SDS-PAGE gel.

  • Paraffin-embedded Cal27 xenograft tissue stained for Cytokeratin 15 using ab227265 at 1/500 dilution in immunohistochemical analysis.

References

ab227265 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227265.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up