Recombinant
RabMAb

Anti-Cytokeratin 15 antibody [EPR1614Y] (ab52816)

Abreviews (1)Q&A (4)Specific References (12)

Overview

  • Product name
    Anti-Cytokeratin 15 antibody [EPR1614Y]
    See all Cytokeratin 15 primary antibodies
  • Description
    Rabbit monoclonal [EPR1614Y] to Cytokeratin 15
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, Flow Cyt, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Cytokeratin 15 aa 400-500 (C terminal). The exact sequence is proprietary.

  • Positive control
    • Fetal thymus lysate, human squamous cervical carcinoma, and HeLa cells.
  • General notes

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)
    KD = 5.60 x 10 -10 M
    Learn more about KD
  • Storage buffer
    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR1614Y
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab52816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.
WB 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
IHC-P Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Tissue specificity
      Expressed in a discontinuous manner in the basal cell layer of adult skin epidermis, but continuously in the basal layer of fetal skin epidermis and nail. Also expressed in the outer root sheath above the hair bulb in hair follicle (at protein level). Expressed homogeneously in all cell layers of the esophagus and exocervix, but detected in the basal cell layer only of oral mucosa, skin and in the basal plus the next two layers of the suprabasal epithelium of the palate.
    • Sequence similarities
      Belongs to the intermediate filament family.
    • Information by UniProt
    • Database links
      • Alternative names
        • AI528832 antibody
        • CK 15 antibody
        • CK-15 antibody
        • CK15 antibody
        • Cytokeratin-15 antibody
        • Cytokeratin15 antibody
        • K15 antibody
        • K1C15_HUMAN antibody
        • K1CO antibody
        • Ka15 antibody
        • Keratin 15 antibody
        • Keratin 15 basic antibody
        • Keratin 15 beta antibody
        • Keratin antibody
        • Keratin complex 1 acidic gene 15 antibody
        • Keratin type I cytoskeletal 15 antibody
        • Keratin-15 antibody
        • Keratin15 antibody
        • KRT 15 antibody
        • Krt1-15 antibody
        • KRT15 antibody
        • KRTB antibody
        • KRTL15 antibody
        • Type I cytoskeletal 15 antibody
        • Type I keratin Ka15 antibody
        see all

      Images

      • Western blot - Anti-Cytokeratin 15 antibody [EPR1614Y] (ab52816)
        Western blot - Anti-Cytokeratin 15 antibody [EPR1614Y] (ab52816)
        Anti-Cytokeratin 15 antibody [EPR1614Y] (ab52816) at 1/10000 dilution + fetal thymus lysate at 10 µg

        Secondary
        goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 45 kDa
        Observed band size: 45 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 15 antibody [EPR1614Y] (ab52816)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 15 antibody [EPR1614Y] (ab52816)
        ab52816 at 1/100 dilution staining Cytokeratin 15 in human squamous cervical carcinoma by Immunohistochemistry, Paraffin embedded tissue.
      • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 15 antibody [EPR1614Y] (ab52816)
        Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 15 antibody [EPR1614Y] (ab52816)
        ab52816 at 1/50 dilution staining Cytokeratin 15 in HeLa cells by Immunocytochemistry.
      • Flow Cytometry - Anti-Cytokeratin 15 antibody [EPR1614Y] (ab52816)
        Flow Cytometry - Anti-Cytokeratin 15 antibody [EPR1614Y] (ab52816)
        Overlay histogram showing A431 cells stained with ab52816 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52816,1/100 dilution ) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was was rabbit IgG (monoclonal) ( 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Triton used under the same conditions.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 15 antibody [EPR1614Y] (ab52816)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 15 antibody [EPR1614Y] (ab52816)
        Fluorescent immunohistochemical analysis of paraffin-embedded human normal skin tissue using ab52816. Green-CK15 red-PI

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 15 antibody [EPR1614Y] (ab52816)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 15 antibody [EPR1614Y] (ab52816)
        Fluorescent immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue using ab52816. Green-CK15 red-PI.

      • Other - Anti-Cytokeratin 15 antibody [EPR1614Y] (ab52816)
        Other - Anti-Cytokeratin 15 antibody [EPR1614Y] (ab52816)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

      References

      This product has been referenced in:
      • Gouveia RM  et al. Controlling the 3D architecture of Self-Lifting Auto-generated Tissue Equivalents (SLATEs) for optimized corneal graft composition and stability. Biomaterials 121:205-219 (2017). IF ; Human . Read more (PubMed: 28092777) »
      • Wang P  et al. Basic fibroblast growth factor reduces scar by inhibiting the differentiation of epidermal stem cells to myofibroblasts via the Notch1/Jagged1 pathway. Stem Cell Res Ther 8:114 (2017). Flow Cyt ; Rat . Read more (PubMed: 28511663) »

      See all 12 Publications for this product

      Publishing research using ab52816? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      5 reviews or Q&A

      10
      Votes: Highest First

      Thanks for your e.mail .. I am asking .. can I used them immunofluorescence (IF) for paraffin embedding section ..

      Do you have protocol start with ..

      Many thanks

      Thank you for your reply.

      I have to confirm that we did not test this antibody in IF with paraffin embedded sections.

      This is in theory possible. Although often high background occurs with this method.

      Please follow this l...

      Read More

      Dear Sir/ Madam

      I ordered the following antibodies:

      1) anti-cytokeratin 15 antibodies9(EPR1614Y).(AB52816)



      2) anti-BCRP/ABCG2 antibody (BXP-53).(ab24115)



      I am wondering whether those a...

      Read More

      Thank you for your inquiry.

      I am happy to confirm that both antibodies are tested and guarantee for IHC-P.

      1.) We used ab52816 successfully in a dilution of 1/100 in IHC-P with human squamous cervical carcinoma by Immunohistochemist...

      Read More

      Dear Abcam,   I purchased a Rabbit monoclonal [EPR1614Y] to cytokeratin 15 (ab52816) four months ago. I aliquoted smaller volumes and stored at -20C as per your data sheet and kept some at 4C. When the aliquot at 4C finished I started using the ones ...

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      Thank you for again confirming these details and for your cooperation.   As requested, I have issued a free of charge replacement with the order number xxxx. To check the status of the order please contact our Customer Service team and reference th...

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      Dear Abcam,   Thank you for replying.   I do not have the order or product number but I bought it this summer. I use standard western blot procedure using nitrocellulose membrane, I use 5% milk for blocking for 15 mins and also 5% milk for primary ...

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      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has ca...

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      Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytokeratin 15 antibody [EPR1614Y]

       Inconclusive
      Abreviews
      Abcam guarantees this product to work in the species/application used in this Abreview.
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (skin section)
      Specification
      skin section
      Fixative
      Methanol
      Permeabilization
      Yes - acetone
      Blocking step
      BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rt°C
      Read More
      Username

      Ms. Cinzia Chinnici

      Verified customer

      Submitted Aug 20 2009

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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