Overview

  • Product name

    Anti-Cytokeratin 16/K16 antibody
    See all Cytokeratin 16/K16 primary antibodies
  • Description

    Rabbit polyclonal to Cytokeratin 16/K16
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human Cytokeratin 16/K16 aa 344-473 (C terminal).
    Database link: P08779

  • Positive control

    • HeLa whole cell lysate; Human H441 Xenograft tissue.
  • General notes

    Previously labelled as Cytokeratin 16. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 51 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Tissue specificity

    Expressed in the hair follicle, nail bed and in mucosal stratified squamous epithelia and, suprabasally, in oral epithelium and palmoplantar epidermis. Also found in luminal cells of sweat and mammary gland ducts.
  • Involvement in disease

    Defects in KRT16 are a cause of pachyonychia congenita type 1 (PC1) [MIM:167200]; also known as Jadassohn-Lewandowsky syndrome. PC1 is an autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy resulting in onchyogryposis (thickening and increase in curvature of the nail), palmoplantar keratoderma, follicular hyperkeratosis, and oral leukokeratosis. Hyperhidrosis of the hands and feet is usually present.
    Defects in KRT16 are the cause of palmoplantar keratoderma non-epidermolytic focal (FNEPPK) [MIM:613000]. A dermatological disorder characterized by non-epidermolytic palmoplantar keratoderma limited to the pressure points on the balls of the feet, with later mild involvement on the palms. Oral, genital and follicular keratotic lesions are often present.
    Defects in KRT16 are a cause of unilateral palmoplantar verrucous nevus (UPVN) [MIM:144200]. UPVN is characterized by a localized thickening of the skin in parts of the right palm and the right sole.
    Note=KRT16 and KRT17 are coexpressed only in pathological situations such as metaplasias and carcinomas of the uterine cervix and in psoriasis vulgaris.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Information by UniProt
  • Database links

  • Alternative names

    • CK 16 antibody
    • CK-16 antibody
    • CK16 antibody
    • Cytokeratin 16 antibody
    • Cytokeratin-16 antibody
    • Cytokeratin16 antibody
    • FNEPPK antibody
    • Focal non epidermolytic palmoplantar keratoderma antibody
    • K 16 antibody
    • K16 antibody
    • K1C16_HUMAN antibody
    • K1CP antibody
    • Keratin 1 type I antibody
    • Keratin 16 antibody
    • Keratin antibody
    • Keratin type I cytoskeletal 16 antibody
    • Keratin-16 antibody
    • Keratin16 antibody
    • KRT 16 antibody
    • Krt16 antibody
    • KRT16A antibody
    • NEPPK antibody
    • PC1 antibody
    • type I cytoskeletal 16 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human H441 xenograft tissue, labeling Cytokeratin 16/K16 with ab154361 at 1/100 dilution.

  • Anti-Cytokeratin 16/K16 antibody (ab154361) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 51 kDa



    10% SDS PAGE

References

ab154361 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6
Permeabilization
No
Specification
Mouse skin
Blocking step
Serum as blocking agent for 5 minute(s) · Concentration: 2.5% · Temperature: RT°C
Fixative
Formaldehyde

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Verified customer

Submitted Jul 06 2016

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