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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class I Cytokeratins
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RecombinantRabMAb

Recombinant Anti-Cytokeratin 16/K16 antibody [EP1615Y] - BSA and Azide free (ab232522)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 16/K16 antibody [EP1615Y] - BSA and Azide free (ab232522)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 16/K16 antibody [EP1615Y] - BSA and Azide free (ab232522)
  • Flow Cytometry - Anti-Cytokeratin 16/K16 antibody [EP1615Y] - BSA and Azide free (ab232522)
  • Flow Cytometry - Anti-Cytokeratin 16/K16 antibody [EP1615Y] - BSA and Azide free (ab232522)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 16/K16 antibody [EP1615Y] - BSA and Azide free (ab232522)
  • Anti-Cytokeratin 16/K16 antibody [EP1615Y] - BSA and Azide free (ab232522)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1615Y] to Cytokeratin 16/K16 - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Human

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Recombinant Human Cytokeratin 16/K16 protein (ab114405)

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Overview

  • Product name

    Anti-Cytokeratin 16/K16 antibody [EP1615Y] - BSA and Azide free
    See all Cytokeratin 16/K16 primary antibodies
  • Description

    Rabbit monoclonal [EP1615Y] to Cytokeratin 16/K16 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Cytokeratin 16/K16 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P08779

  • Positive control

    • IHC-P: Human skin tissue.
  • General notes

    ab232522 is the carrier-free version of ab76416 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab232522 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Previously labelled as Cytokeratin 16.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.20
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1615Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class I
    • Cytokeratins

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-Cytokeratin 16/K16 antibody [EP1615Y] (ab205744)
    • Alexa Fluor® 647 Anti-Cytokeratin 16/K16 antibody [EP1615Y] (ab205745)
    • HRP Anti-Cytokeratin 16/K16 antibody [EP1615Y] (ab206064)
    • Alexa Fluor® 555 Anti-Cytokeratin 16/K16 antibody [EP1615Y] (ab215292)
    • Anti-Cytokeratin 16/K16 antibody [EP1615Y] (ab76416)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Cytokeratin 16/K16 protein (ab114405)
  • Related Products

    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)

Applications

Our Abpromise guarantee covers the use of ab232522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 51 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.

Target

  • Tissue specificity

    Expressed in the hair follicle, nail bed and in mucosal stratified squamous epithelia and, suprabasally, in oral epithelium and palmoplantar epidermis. Also found in luminal cells of sweat and mammary gland ducts.
  • Involvement in disease

    Defects in KRT16 are a cause of pachyonychia congenita type 1 (PC1) [MIM:167200]; also known as Jadassohn-Lewandowsky syndrome. PC1 is an autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy resulting in onchyogryposis (thickening and increase in curvature of the nail), palmoplantar keratoderma, follicular hyperkeratosis, and oral leukokeratosis. Hyperhidrosis of the hands and feet is usually present.
    Defects in KRT16 are the cause of palmoplantar keratoderma non-epidermolytic focal (FNEPPK) [MIM:613000]. A dermatological disorder characterized by non-epidermolytic palmoplantar keratoderma limited to the pressure points on the balls of the feet, with later mild involvement on the palms. Oral, genital and follicular keratotic lesions are often present.
    Defects in KRT16 are a cause of unilateral palmoplantar verrucous nevus (UPVN) [MIM:144200]. UPVN is characterized by a localized thickening of the skin in parts of the right palm and the right sole.
    Note=KRT16 and KRT17 are coexpressed only in pathological situations such as metaplasias and carcinomas of the uterine cervix and in psoriasis vulgaris.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Target information above from: UniProt accession P08779 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3868 Human
    • Omim: 148067 Human
    • SwissProt: P08779 Human
    • Unigene: 655160 Human
    • Alternative names

      • CK 16 antibody
      • CK-16 antibody
      • CK16 antibody
      • Cytokeratin 16 antibody
      • Cytokeratin-16 antibody
      • Cytokeratin16 antibody
      • FNEPPK antibody
      • Focal non epidermolytic palmoplantar keratoderma antibody
      • K 16 antibody
      • K16 antibody
      • K1C16_HUMAN antibody
      • K1CP antibody
      • Keratin 1 type I antibody
      • Keratin 16 antibody
      • Keratin antibody
      • Keratin type I cytoskeletal 16 antibody
      • Keratin-16 antibody
      • Keratin16 antibody
      • KRT 16 antibody
      • Krt16 antibody
      • KRT16A antibody
      • NEPPK antibody
      • PC1 antibody
      • type I cytoskeletal 16 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 16/K16 antibody [EP1615Y] - BSA and Azide free (ab232522)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 16/K16 antibody [EP1615Y] - BSA and Azide free (ab232522)

      ab76416 staining Cytokeratin 16/K16 in human skeletal muscle sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

      Negative control 1: PBS in place of primary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76416).

    • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 16/K16 antibody [EP1615Y] - BSA and Azide free (ab232522)
      Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 16/K16 antibody [EP1615Y] - BSA and Azide free (ab232522)

      ab76416 staining Cytokeratin 16/K16 in A431 (human epidermoid carcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody. ab7291 and ab150120 were used as counterstains for primary antibody ab75748 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

      Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
      Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)

       

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76416).

    • Flow Cytometry - Anti-Cytokeratin 16/K16 antibody [EP1615Y] - BSA and Azide free (ab232522)
      Flow Cytometry - Anti-Cytokeratin 16/K16 antibody [EP1615Y] - BSA and Azide free (ab232522)

      ab76416 staining Cytokeratin 16 / K16 in HACAT (human keratinocyte) cell line by flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/150. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/500 was used as the secondary antibody.

      Isoytype control: Rabbit monoclonal IgG (Black)

      Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

       

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76416).

    • Flow Cytometry - Anti-Cytokeratin 16/K16 antibody [EP1615Y] - BSA and Azide free (ab232522)
      Flow Cytometry - Anti-Cytokeratin 16/K16 antibody [EP1615Y] - BSA and Azide free (ab232522)

      Overlay histogram showing HepG2 cells stained with unpurified ab76416 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76416, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Triton used under the same conditions.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76416).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 16/K16 antibody [EP1615Y] - BSA and Azide free (ab232522)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 16/K16 antibody [EP1615Y] - BSA and Azide free (ab232522)

      ab76416 staining Cytokeratin 16/K16 in human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

      Negative control 1: PBS in place of primary antibody.

       This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76416).

    • Anti-Cytokeratin 16/K16 antibody [EP1615Y] - BSA and Azide free (ab232522)
      Anti-Cytokeratin 16/K16 antibody [EP1615Y] - BSA and Azide free (ab232522)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab232522? Please let us know so that we can cite the reference in this datasheet.

    ab232522 has not yet been referenced specifically in any publications.

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