Product nameAnti-Cytokeratin 16/K16 antibody [EP1615Y] (HRP)
See all Cytokeratin 16/K16 primary antibodies
DescriptionRabbit monoclonal [EP1615Y] to Cytokeratin 16/K16 (HRP)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Cytokeratin 16/K16 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P08779
- WB: HaCaT whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.4
Preservative: 0.1% Proclin
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
- Anti-Cytokeratin 16/K16 antibody [EP1615Y] (Alexa Fluor® 488) (ab205744)
- Anti-Cytokeratin 16/K16 antibody [EP1615Y] (Alexa Fluor® 647) (ab205745)
- Anti-Cytokeratin 16/K16 antibody [EP1615Y] (Alexa Fluor® 555) (ab215292)
- Anti-Cytokeratin 16/K16 antibody [EP1615Y] - BSA and Azide free (ab232522)
- Anti-Cytokeratin 16/K16 antibody [EP1615Y] (ab76416)
Our Abpromise guarantee covers the use of ab206064 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).|
Tissue specificityExpressed in the hair follicle, nail bed and in mucosal stratified squamous epithelia and, suprabasally, in oral epithelium and palmoplantar epidermis. Also found in luminal cells of sweat and mammary gland ducts.
Involvement in diseaseDefects in KRT16 are a cause of pachyonychia congenita type 1 (PC1) [MIM:167200]; also known as Jadassohn-Lewandowsky syndrome. PC1 is an autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy resulting in onchyogryposis (thickening and increase in curvature of the nail), palmoplantar keratoderma, follicular hyperkeratosis, and oral leukokeratosis. Hyperhidrosis of the hands and feet is usually present.
Defects in KRT16 are the cause of palmoplantar keratoderma non-epidermolytic focal (FNEPPK) [MIM:613000]. A dermatological disorder characterized by non-epidermolytic palmoplantar keratoderma limited to the pressure points on the balls of the feet, with later mild involvement on the palms. Oral, genital and follicular keratotic lesions are often present.
Defects in KRT16 are a cause of unilateral palmoplantar verrucous nevus (UPVN) [MIM:144200]. UPVN is characterized by a localized thickening of the skin in parts of the right palm and the right sole.
Note=KRT16 and KRT17 are coexpressed only in pathological situations such as metaplasias and carcinomas of the uterine cervix and in psoriasis vulgaris.
Sequence similaritiesBelongs to the intermediate filament family.
- Information by UniProt
- CK 16 antibody
- CK-16 antibody
- CK16 antibody
Anti-Cytokeratin 16/K16 antibody [EP1615Y] (HRP) (ab206064) at 1/5000 dilution + HaCaT (Human keratinocyte cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 51 kDa
Exposure time: 16 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab206064 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab206064 has not yet been referenced specifically in any publications.