Overview

  • Product name

    Anti-Cytokeratin 16/K16 antibody [EPR13504]
    See all Cytokeratin 16/K16 primary antibodies
  • Description

    Rabbit monoclonal [EPR13504] to Cytokeratin 16/K16
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Cytokeratin 16/K16 aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: P08779

  • Positive control

    • HeLa and Human skin lysates; Human skin and tonsil tissues.
  • General notes

     

     

    Previously labelled as Cytokeratin 16. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR13504
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/100000. Detects a band of approximately 48 kDa (predicted molecular weight: 51 kDa).
IHC-P 1/250 - 1/500.

Target

  • Tissue specificity

    Expressed in the hair follicle, nail bed and in mucosal stratified squamous epithelia and, suprabasally, in oral epithelium and palmoplantar epidermis. Also found in luminal cells of sweat and mammary gland ducts.
  • Involvement in disease

    Defects in KRT16 are a cause of pachyonychia congenita type 1 (PC1) [MIM:167200]; also known as Jadassohn-Lewandowsky syndrome. PC1 is an autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy resulting in onchyogryposis (thickening and increase in curvature of the nail), palmoplantar keratoderma, follicular hyperkeratosis, and oral leukokeratosis. Hyperhidrosis of the hands and feet is usually present.
    Defects in KRT16 are the cause of palmoplantar keratoderma non-epidermolytic focal (FNEPPK) [MIM:613000]. A dermatological disorder characterized by non-epidermolytic palmoplantar keratoderma limited to the pressure points on the balls of the feet, with later mild involvement on the palms. Oral, genital and follicular keratotic lesions are often present.
    Defects in KRT16 are a cause of unilateral palmoplantar verrucous nevus (UPVN) [MIM:144200]. UPVN is characterized by a localized thickening of the skin in parts of the right palm and the right sole.
    Note=KRT16 and KRT17 are coexpressed only in pathological situations such as metaplasias and carcinomas of the uterine cervix and in psoriasis vulgaris.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Information by UniProt
  • Database links

  • Alternative names

    • CK 16 antibody
    • CK-16 antibody
    • CK16 antibody
    • Cytokeratin 16 antibody
    • Cytokeratin-16 antibody
    • Cytokeratin16 antibody
    • FNEPPK antibody
    • Focal non epidermolytic palmoplantar keratoderma antibody
    • K 16 antibody
    • K16 antibody
    • K1C16_HUMAN antibody
    • K1CP antibody
    • Keratin 1 type I antibody
    • Keratin 16 antibody
    • Keratin antibody
    • Keratin type I cytoskeletal 16 antibody
    • Keratin-16 antibody
    • Keratin16 antibody
    • KRT 16 antibody
    • Krt16 antibody
    • KRT16A antibody
    • NEPPK antibody
    • PC1 antibody
    • type I cytoskeletal 16 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Cytokeratin 16/K16 with ab181055 at 1/500 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

  • Anti-Cytokeratin 16/K16 antibody [EPR13504] (ab181055) at 1/100000 dilution + Human skin lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 51 kDa

  • Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling Cytokeratin 16/K16 with ab181055 at 1/500 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

  • Anti-Cytokeratin 16/K16 antibody [EPR13504] (ab181055) at 1/100000 dilution + HeLa cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L) HRP at 1/1000 dilution

    Predicted band size: 51 kDa

References

ab181055 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181055.
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