Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1624Y] to Cytokeratin 17
- Suitable for: Flow Cyt, ICC/IF, WB, IP, IHC-P
- Reacts with: Human
Product nameAnti-Cytokeratin 17 antibody [EPR1624Y]
See all Cytokeratin 17 primary antibodies
DescriptionRabbit monoclonal [EPR1624Y] to Cytokeratin 17
SpecificityThe immunogen used for this product shares 78.6% homology with KRT36 and KRT24. Cross-reactivity with these proteins has not been confirmed experimentally.
Tested applicationsSuitable for: Flow Cyt, ICC/IF, WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Cytokeratin 17 aa 400-500 (C terminal). The exact sequence is proprietary.
- WB: HeLa cell lysate IHC-P: Human cervical carcinoma tissue and Human squamous cervical carcinoma tissue ICC/IF: A431 cells. Flow Cyt: HeLa cells IP: HeLa cell lysates
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Dissociation constant (KD)KD = 2.47 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, 59% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab51056 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
For unpurified use at 1/50 - 1/100
|WB||1/50000. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa).
For unpurified use at 1/10000.
For unpurified use at 1/30
|IHC-P||1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
For unpurified use at 1/50 - 1/100. See IHC antigen retrieval protocols.
FunctionMay play a role in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair. May be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial "stem cells". May act as an autoantigen in the immunopathogenesis of psoriasis, with certain peptide regions being a major target for autoreactive T-cells and hence causing their proliferation. Required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state. Modulates the function of TNF-alpha in the specific context of hair cycling. Regulates protein synthesis and epithelial cell growth through binding to the adapter protein SFN and by stimulating Akt/mTOR pathway. Involved in tissue repair.
Tissue specificityExpressed in the outer root sheath and medulla region of hair follicle specifically from eyebrow and beard, digital pulp, nail matrix and nail bed epithelium, mucosal stratified squamous epithelia and in basal cells of oral epithelium, palmoplantar epidermis and sweat and mammary glands. Also expressed in myoepithelium of prostate, basal layer of urinary bladder, cambial cells of sebaceous gland and in exocervix (at protein level).
Involvement in diseaseDefects in KRT17 are a cause of pachyonychia congenita type 2 (PC2) [MIM:167210]; also known as pachyonychia congenita Jackson-Lawler type. PC2 is an autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy resulting in onchyogryposis (thickening and increase in curvature of the nail), palmoplantar keratoderma and hyperhidrosis, follicular hyperkeratosis, multiple epidermal cysts, absent/sparse eyebrow and body hair, and by the presence of natal teeth.
Defects in KRT17 are a cause of steatocystoma multiplex (SM) [MIM:184500]. SM is a disease characterized by round or oval cystic tumors widely distributed on the back, anterior trunk, arms, scrotum, and thighs.
Note=KRT16 and KRT17 are coexpressed only in pathological situations such as metaplasias and carcinomas of the uterine cervix and in psoriasis vulgaris.
Sequence similaritiesBelongs to the intermediate filament family.
- Information by UniProt
- 39.1 antibody
- CK 17 antibody
- CK-17 antibody
Anti-Cytokeratin 17 antibody [EPR1624Y] (ab51056) at 1/10000 dilution (Unpurified) + HeLa cell lysate at 10 µg
Goat anti-Rabbit HRP labeled at 1/2000 dilution
Predicted band size: 48 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence analysis of A431 (human epidermoid carcinoma epithelial cell) cells labeling Cytokeratin 17 with Purified ab51056 at 1:250 dilution (0.4 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
ab51056 (purified) at 1:20 dilution (1µg) immunoprecipitating Cytokeratin 17 in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab51056 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab51056 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cytokeratin 17 with Purified ab51056 at 1:20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cervical cancer tissue sections labeling Cytokeratin 17 with Purified ab51056 at 1:400 dilution (0.22 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
Unpurified ab51056 (1/50) staining human Cytokeratin 17 in paraffin embedded human squamous cervical carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunofluorescent staining of A431 cells using unpurified ab51056 (1/50).
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab51056 has been referenced in 6 publications.
- Su Y et al. Pre-aggregation of scalp progenitor dermal and epidermal stem cells activates the WNT pathway and promotes hair follicle formation in in vitro and in vivo systems. Stem Cell Res Ther 10:403 (2019). PubMed: 31856904
- Ananthula S et al. Geminin overexpression-dependent recruitment and crosstalk with mesenchymal stem cells enhance aggressiveness in triple negative breast cancers. Oncotarget 7:20869-89 (2016). PubMed: 26989079
- Khanom R et al. Keratin 17 Is Induced in Oral Cancer and Facilitates Tumor Growth. PLoS One 11:e0161163 (2016). PubMed: 27512993
- Ziegler YS et al. Plasma membrane proteomics of human breast cancer cell lines identifies potential targets for breast cancer diagnosis and treatment. PLoS One 9:e102341 (2014). WB, ICC/IF ; Human . PubMed: 25029196
- Fulzele A et al. Proteomic profile of keratins in cancer of the gingivo buccal complex: Consolidating insights for clinical applications. J Proteomics 91C:242-258 (2013). WB, IHC-P ; Human . PubMed: 23876858
- Shimizu Y et al. BRCA1/p220 loss triggers BRCA1-IRIS overexpression via mRNA stabilization in breast cancer cells. Oncotarget 3:299-313 (2012). WB . PubMed: 22431556