Overview

  • Product name

    Anti-Cytokeratin 17 antibody [SP95] - C-terminal
    See all Cytokeratin 17 primary antibodies
  • Description

    Rabbit monoclonal [SP95] to Cytokeratin 17 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide within Human Cytokeratin 17 aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q04695

  • Positive control

    • IHC-P: Human prostate carcinoma, Human cervix. Human tonsil, Human thymus, Human cervix adenocarcinoma, Human cervical adenocarcinoma, Human bladder, Human bladder transitional cell carcinoma, Human prostate adenocarcinoma, Human prostate, Human skin and Human skin squamous cell carcinoma. ICC/IF: HeLa FC: HeLa WB: HeLa cell lysate
  • General notes

     

     

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.6
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from TCS by protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    SP95
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100. Predicted molecular weight: 48 kDa.
Flow Cyt 1/20 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    May play a role in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair. May be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial "stem cells". May act as an autoantigen in the immunopathogenesis of psoriasis, with certain peptide regions being a major target for autoreactive T-cells and hence causing their proliferation. Required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state. Modulates the function of TNF-alpha in the specific context of hair cycling. Regulates protein synthesis and epithelial cell growth through binding to the adapter protein SFN and by stimulating Akt/mTOR pathway. Involved in tissue repair.
  • Tissue specificity

    Expressed in the outer root sheath and medulla region of hair follicle specifically from eyebrow and beard, digital pulp, nail matrix and nail bed epithelium, mucosal stratified squamous epithelia and in basal cells of oral epithelium, palmoplantar epidermis and sweat and mammary glands. Also expressed in myoepithelium of prostate, basal layer of urinary bladder, cambial cells of sebaceous gland and in exocervix (at protein level).
  • Involvement in disease

    Defects in KRT17 are a cause of pachyonychia congenita type 2 (PC2) [MIM:167210]; also known as pachyonychia congenita Jackson-Lawler type. PC2 is an autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy resulting in onchyogryposis (thickening and increase in curvature of the nail), palmoplantar keratoderma and hyperhidrosis, follicular hyperkeratosis, multiple epidermal cysts, absent/sparse eyebrow and body hair, and by the presence of natal teeth.
    Defects in KRT17 are a cause of steatocystoma multiplex (SM) [MIM:184500]. SM is a disease characterized by round or oval cystic tumors widely distributed on the back, anterior trunk, arms, scrotum, and thighs.
    Note=KRT16 and KRT17 are coexpressed only in pathological situations such as metaplasias and carcinomas of the uterine cervix and in psoriasis vulgaris.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 39.1 antibody
    • CK 17 antibody
    • CK-17 antibody
    • Cytokeratin-17 antibody
    • K17 antibody
    • K1C17_HUMAN antibody
    • Keratin 17 antibody
    • keratin 17 epitope S1 antibody
    • keratin 17 epitope S2 antibody
    • keratin 17 epitope S4 antibody
    • Keratin 17, type I antibody
    • Keratin antibody
    • Keratin type I cytoskeletal 17 antibody
    • keratin, type i cytoskeletal 17 [version 1] antibody
    • Keratin-17 antibody
    • KRT17 antibody
    • PC antibody
    • PC2 antibody
    • PCHC1 antibody
    • type I cytoskeletal 17 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded Human skin squamous cell carcinoma tissue labeling Cytokeratin 17 with ab183330 at 1/100.

  • Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling Cytokeratin 17 with purified ab183330 at 1/100 (1.6µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow cytometry analysis of Hela (human cervix adenocarcinoma) labeling Cytokeratin 17 with purified ab183330 at 1/20 dilution (7.85 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlableled control - Unlabelled cells (blue).

  • Anti-Cytokeratin 17 antibody [SP95] - C-terminal (ab183330) at 1/25 dilution + HeLa cell lysate

    Predicted band size: 48 kDa

  • Flow cytometric analysis of Cytokeratin HeLa cells using ab183330 (green) compare to negative control of rabbit IgG (blue).

  • Immunohistochemical analysis of paraffin embedded Human skin tissue labeling Cytokeratin 17 with ab183330 at 1/100.

  • Immunohistochemical analysis of paraffin embedded Human prostate tissue labeling Cytokeratin 17 with ab183330 at 1/100.

  • Immunohistochemical analysis of paraffin embedded Human prostate adenocarcinoma tissue labeling Cytokeratin 17 with ab183330 at 1/100.

  • Immunohistochemical analysis of paraffin embedded Human bladder transitional cell carcinoma tissue labeling Cytokeratin 17 with ab183330 at 1/100.

  • Immunohistochemical analysis of paraffin embedded Human bladder tissue labeling Cytokeratin 17 with ab183330 at 1/100.

  • Immunohistochemical analysis of paraffin embedded Human cervical adenocarcinoma tissue labeling Cytokeratin 17 with ab183330 at 1/100.

  • Immunohistochemical analysis of paraffin embedded Human cervix adenocarcinoma tissue labeling Cytokeratin 17 with ab183330 at 1/100.

  • Immunohistochemical analysis of paraffin embedded Human thymus tissue labeling Cytokeratin 17 with ab183330 at 1/100.

  • Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling Cytokeratin 17 with ab183330 at 1/100.

  • Immunohistochemical analysis of paraffin embedded Human cervix tissue labeling Cytokeratin 17 with ab183330 at 1/100.

  • Immunohistochemical analysis of paraffin embedded Human prostate carcinoma tissue labeling Cytokeratin 17 with ab183330 at 1/100.

References

ab183330 has not yet been referenced specifically in any publications.

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