Overview

  • Product name

    Anti-Cytokeratin 17 antibody [SPM560]
    See all Cytokeratin 17 primary antibodies
  • Description

    Mouse monoclonal [SPM560] to Cytokeratin 17
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Rat, Goat, Cow, Human, Pig
  • Immunogen

    Tissue, cells or virus corresponding to Rat Cytokeratin 17.

  • Positive control

    • WB: HeLa whole cell lysate. IHC-P: Human skin tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituents: PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from Bioreactor Concentrate by Protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    SPM560
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab233912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Incubate with primary antibody for 30 minutes at RT.

WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 48 kDa.

Target

  • Function

    May play a role in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair. May be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial "stem cells". May act as an autoantigen in the immunopathogenesis of psoriasis, with certain peptide regions being a major target for autoreactive T-cells and hence causing their proliferation. Required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state. Modulates the function of TNF-alpha in the specific context of hair cycling. Regulates protein synthesis and epithelial cell growth through binding to the adapter protein SFN and by stimulating Akt/mTOR pathway. Involved in tissue repair.
  • Tissue specificity

    Expressed in the outer root sheath and medulla region of hair follicle specifically from eyebrow and beard, digital pulp, nail matrix and nail bed epithelium, mucosal stratified squamous epithelia and in basal cells of oral epithelium, palmoplantar epidermis and sweat and mammary glands. Also expressed in myoepithelium of prostate, basal layer of urinary bladder, cambial cells of sebaceous gland and in exocervix (at protein level).
  • Involvement in disease

    Defects in KRT17 are a cause of pachyonychia congenita type 2 (PC2) [MIM:167210]; also known as pachyonychia congenita Jackson-Lawler type. PC2 is an autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy resulting in onchyogryposis (thickening and increase in curvature of the nail), palmoplantar keratoderma and hyperhidrosis, follicular hyperkeratosis, multiple epidermal cysts, absent/sparse eyebrow and body hair, and by the presence of natal teeth.
    Defects in KRT17 are a cause of steatocystoma multiplex (SM) [MIM:184500]. SM is a disease characterized by round or oval cystic tumors widely distributed on the back, anterior trunk, arms, scrotum, and thighs.
    Note=KRT16 and KRT17 are coexpressed only in pathological situations such as metaplasias and carcinomas of the uterine cervix and in psoriasis vulgaris.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 39.1 antibody
    • CK 17 antibody
    • CK-17 antibody
    • Cytokeratin-17 antibody
    • K17 antibody
    • K1C17_HUMAN antibody
    • Keratin 17 antibody
    • keratin 17 epitope S1 antibody
    • keratin 17 epitope S2 antibody
    • keratin 17 epitope S4 antibody
    • Keratin 17, type I antibody
    • Keratin antibody
    • Keratin type I cytoskeletal 17 antibody
    • keratin, type i cytoskeletal 17 [version 1] antibody
    • Keratin-17 antibody
    • KRT17 antibody
    • PC antibody
    • PC2 antibody
    • PCHC1 antibody
    • type I cytoskeletal 17 antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human skin tissue stained for Cytokeratin 17 using ab233912 at 1 μg/ml in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human skin tissue stained for Cytokeratin 17 using ab233912 at 1 μg/ml in immunohistochemical analysis.

  • Anti-Cytokeratin 17 antibody [SPM560] (ab233912) at 1 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Developed using the ECL technique.

    Predicted band size: 48 kDa

References

ab233912 has not yet been referenced specifically in any publications.

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