Overview

  • Product name

    Anti-Cytokeratin 18 antibody
    See all Cytokeratin 18 primary antibodies
  • Description

    Rabbit polyclonal to Cytokeratin 18
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 164-425 of Human Cytokeratin 18 (NP_000215, NP_954657).

  • Positive control

    • Recombinant Human Cytokeratin 18 protein (ab73638) can be used as a positive control in WB. HeLa cells and whole cell lysate, A431, H1299, HepG2, Molt4, Raji whole cell lysates, H661 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab137860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)
WB 1/5000 - 1/20000. Predicted molecular weight: 48 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • Tissue specificity

    Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
  • Involvement in disease

    Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Post-translational
    modifications

    Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
  • Cellular localization

    Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cell proliferation inducing gene 46 protein antibody
    • Cell proliferation inducing protein 46 antibody
    • Cell proliferation-inducing gene 46 protein antibody
    • CK 18 antibody
    • CK-18 antibody
    • CK18 antibody
    • CYK 18 antibody
    • CYK18 antibody
    • Cytokeratin 18 antibody
    • Cytokeratin endo B antibody
    • Cytokeratin-18 antibody
    • K 18 antibody
    • K18 antibody
    • K1C18_HUMAN antibody
    • KA18 antibody
    • Keratin 18 antibody
    • Keratin 18, type I antibody
    • Keratin D antibody
    • keratin, type I cytoskeletal 18 antibody
    • Keratin-18 antibody
    • Krt18 antibody
    see all

Images

  • Immunofluorescence analysis of paraformaldehyde fixed HeLa cells labelling Cytokeratin 18 with ab137860 at 1/200. Lower panel shows mergence with DNA probe.
  • Anti-Cytokeratin 18 antibody (ab137860) at 1/10000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 48 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin embedded H661 xenograft tissue labelling Cytokeratin 18 with ab137860 at 1/500.

References

ab137860 has not yet been referenced specifically in any publications.

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