Overview

  • Product name

    Anti-Cytokeratin 18 antibody
    See all Cytokeratin 18 primary antibodies
  • Description

    Goat polyclonal to Cytokeratin 18
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human, Pig
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to Human Cytokeratin 18 aa 265-276 (internal sequence) (Cysteine residue). NP_000215.1.
    Sequence:

    C-EELDKYWSQQIE


    Database link: P05783

  • Positive control

    • WB: HeLa cell lysate; Pig liver lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab223248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 45,48 kDa (predicted molecular weight: 48 kDa).
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function

    Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • Tissue specificity

    Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
  • Involvement in disease

    Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Post-translational
    modifications

    Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
  • Cellular localization

    Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cell proliferation inducing gene 46 protein antibody
    • Cell proliferation inducing protein 46 antibody
    • Cell proliferation-inducing gene 46 protein antibody
    • CK 18 antibody
    • CK-18 antibody
    • CK18 antibody
    • CYK 18 antibody
    • CYK18 antibody
    • Cytokeratin 18 antibody
    • Cytokeratin endo B antibody
    • Cytokeratin-18 antibody
    • K 18 antibody
    • K18 antibody
    • K1C18_HUMAN antibody
    • KA18 antibody
    • Keratin 18 antibody
    • Keratin 18, type I antibody
    • Keratin D antibody
    • keratin, type I cytoskeletal 18 antibody
    • Keratin-18 antibody
    • Krt18 antibody
    see all

Images

  • ab223248 (5 µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

  • Anti-Cytokeratin 18 antibody (ab223248) at 0.1 µg + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 48 kDa



    Primary incubation: 1 hour.

  • ab223248 (5 µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

  • ab223248 (5 µg/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

  • Anti-Cytokeratin 18 antibody (ab223248) at 0.1 µg/ml + Pig liver lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 48 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?



    Primary incubation: 1 hour.

References

ab223248 has not yet been referenced specifically in any publications.

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