• Product name
    Anti-Cytokeratin 18 antibody
    See all Cytokeratin 18 primary antibodies
  • Description
    Rabbit polyclonal to Cytokeratin 18
  • Host species
  • Tested applications
    Suitable for: WB, IP, Flow Cyt, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human Cytokeratin 18 aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
    (Peptide available as ab215576)

  • Positive control
    • A431 cell lysate, human urothelial carcinoma cells and HeLa cells.



Our Abpromise guarantee covers the use of ab52948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IP 1/100.
Flow Cyt 1/15.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.
ICC/IF 1/250 - 1/500.


  • Function
    Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • Tissue specificity
    Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
  • Involvement in disease
    Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Post-translational
    Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
  • Cellular localization
    Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell proliferation inducing gene 46 protein antibody
    • Cell proliferation inducing protein 46 antibody
    • Cell proliferation-inducing gene 46 protein antibody
    • CK 18 antibody
    • CK-18 antibody
    • CK18 antibody
    • CYK 18 antibody
    • CYK18 antibody
    • Cytokeratin 18 antibody
    • Cytokeratin endo B antibody
    • Cytokeratin-18 antibody
    • K 18 antibody
    • K18 antibody
    • K1C18_HUMAN antibody
    • KA18 antibody
    • Keratin 18 antibody
    • Keratin 18, type I antibody
    • Keratin D antibody
    • keratin, type I cytoskeletal 18 antibody
    • Keratin-18 antibody
    • Krt18 antibody
    see all


  • Anti-Cytokeratin 18 antibody (ab52948) at 1/50000 dilution + A431 cell lysate at 10 µg

    goat anti-rabbit HR at 1/2000 dilution

    Predicted band size: 48 kDa
    Observed band size: 48 kDa

  • ab52948 at 1/250 dilution staining Cytokeratin 18 in urothelial carcinoma cells by Immunohistochemistry, Paraffin embedded tissue.
  • ab52948 at 1/250 dilution staining Cytokeratin 18 in HeLa cells by Immunofluorescence


This product has been referenced in:
  • Cruz R  et al. Epithelial Keratins Modulate cMet Expression and Signaling and Promote InlB-Mediated Listeria monocytogenes Infection of HeLa Cells. Front Cell Infect Microbiol 8:146 (2018). Read more (PubMed: 29868502) »
  • Herrick DB  et al. Notch1 maintains dormancy of olfactory horizontal basal cells, a reserve neural stem cell. Proc Natl Acad Sci U S A 114:E5589-E5598 (2017). Read more (PubMed: 28637720) »

See all 15 Publications for this product

Customer reviews and Q&As

Human Recombinant protein (Human Cytokeratin 18 full length protein (ab73638))
Human Cytokeratin 18 full length protein (ab73638)
Sandwich (Capture)
Blocking step
StartingBlock blocking buffer (Thermo Scientific) as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 100% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Feb 25 2015

The cat number is ab133272 and this one has been used for WB and IP.

Thank you very much for your email! We are happy to hear the new vial worked well!

Thank you again for your update and help! I wish you a great evening!

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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I just checked our internal notes and it looks like had we issued a Product recall to inform our customers regarding two faulty batches of this product. The normal procedure is that we through all the effected lot...

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Thanks for your reply. I have let my colleague know and we will send this replacement vial as soon as possible. Due to the holidays, it may take longer than usual. Please let me know if there is anything else that we can do for you.

Thank you for your reply. I am happy that the new vial of this antibody has proven to be successful. The lot from order number ****** was the lot that I sent the free of charge replacement for. The lot number *****, from what I can find out is from 200...

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Thank you for confirming that information. I have sent you a new vial of ab52948 which is from a different lot than you previously received. Your new order number is ********. If there is anything else I can help you with, please let me kn

Thank you for contacting Abcam. I am sorry that you are having problems with ab52948. It does appear there was a problem with the lot that you received (******) and we are in the process of contacting customers and letting them know that we wil...

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Thank you for your reply, and I apologize again for the problem with this antibody. I have issued credit note ***, which will be refunded back to your credit card pending approval from our accounting department. If you have any questions about ...

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