Key features and details
- Rabbit polyclonal to Cytokeratin 18 - C-terminal
- Suitable for: ICC/IF, WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Cytokeratin 18 antibody - C-terminal
See all Cytokeratin 18 primary antibodies
DescriptionRabbit polyclonal to Cytokeratin 18 - C-terminal
Tested applicationsSuitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human Cytokeratin 18 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: P05783
- Human prostate, prostate carcinoma and colon carcinoma tissue. K562, NCI-H460 and mouse stomach cell lysates. HeLa and WiDr cells.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityAmmonium Sulphate Precipitation
Our Abpromise guarantee covers the use of ab189444 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/10 - 1/50.|
|WB||1/1000. Predicted molecular weight: 48 kDa.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionInvolved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Tissue specificityExpressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
Involvement in diseaseDefects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
Sequence similaritiesBelongs to the intermediate filament family.
modificationsPhosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
Cellular localizationCytoplasm > perinuclear region.
- Information by UniProt
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All lanes : Anti-Cytokeratin 18 antibody - C-terminal (ab189444) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : NCI-H460 cell lysate
Lane 3 : Mouse stomach tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 48 kDa
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling Cytokeratin 18 with ab189444 at 5 µg/ml. ab189444 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling Cytokeratin 18 with ab189444 at 5 µg/ml.
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human prostate tissue labeling Cytokeratin 18 with ab189444 at 5 µg/ml.
Immunocytochemistry/ Immunofluorescence analysis of human prostate carcinoma tissue labeling Cytokeratin 18 with ab189444 at 1/10 dilution followed by Alexa Fluor 488-conjugated Goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling Cytokeratin 18 with ab189444 at 1/10 dilution followed by Alexa Fluor 488-conjugated Goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Flow cytometry analysis of WiDr cells using ab189444 at 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated Goat-anti-rabbit secondary antibodies were used for the analysis.
ab189444 has been referenced in 4 publications.
- Paller C et al. TGF-ß receptor I inhibitor enhances response to enzalutamide in a pre-clinical model of advanced prostate cancer. Prostate 79:31-43 (2019). PubMed: 30155899
- Hensley PJ et al. Predictive and targeting value of IGFBP-3 in therapeutically resistant prostate cancer. Am J Clin Exp Urol 7:188-202 (2019). PubMed: 31317059
- Gonzalez ME et al. Mesenchymal Stem Cell-Induced DDR2 Mediates Stromal-Breast Cancer Interactions and Metastasis Growth. Cell Rep 18:1215-1228 (2017). WB ; Human . PubMed: 28147276
- Xiang Y et al. Effect of Yi Guan Jian decoction on differentiation of bone marrow mesenchymalstem cells into hepatocyte-like cells in dimethylnitrosamine-induced liver cirrhosis in mice. Mol Med Rep 15:613-626 (2017). PubMed: 28035356