Overview

  • Product name

    Anti-Cytokeratin 18 antibody - C-terminal
    See all Cytokeratin 18 primary antibodies
  • Description

    Rabbit polyclonal to Cytokeratin 18 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Cytokeratin 18 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P05783

  • Positive control

    • Human prostate, prostate carcinoma and colon carcinoma tissue. K562, NCI-H460 and mouse stomach cell lysates. HeLa and WiDr cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab189444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/10 - 1/50.
WB 1/1000. Predicted molecular weight: 48 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • Tissue specificity

    Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
  • Involvement in disease

    Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Post-translational
    modifications

    Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
  • Cellular localization

    Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cell proliferation inducing gene 46 protein antibody
    • Cell proliferation inducing protein 46 antibody
    • Cell proliferation-inducing gene 46 protein antibody
    • CK 18 antibody
    • CK-18 antibody
    • CK18 antibody
    • CYK 18 antibody
    • CYK18 antibody
    • Cytokeratin 18 antibody
    • Cytokeratin endo B antibody
    • Cytokeratin-18 antibody
    • K 18 antibody
    • K18 antibody
    • K1C18_HUMAN antibody
    • KA18 antibody
    • Keratin 18 antibody
    • Keratin 18, type I antibody
    • Keratin D antibody
    • keratin, type I cytoskeletal 18 antibody
    • Keratin-18 antibody
    • Krt18 antibody
    see all

Images

  • All lanes : Anti-Cytokeratin 18 antibody - C-terminal (ab189444) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : NCI-H460 cell lysate
    Lane 3 : Mouse stomach tissue lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 48 kDa

  • Immunohistochemistry analysis of  formalin-fixed, paraffin-embedded  human colon carcinoma tissue labeling Cytokeratin 18 with ab189444 at 5 µg/ml. ab189444 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

  • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling Cytokeratin 18 with ab189444 at 5 µg/ml.

  • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human prostate tissue labeling Cytokeratin 18 with ab189444 at 5 µg/ml.

  • Immunocytochemistry/ Immunofluorescence analysis of human prostate carcinoma tissue labeling Cytokeratin 18 with ab189444 at 1/10 dilution followed by Alexa Fluor 488-conjugated Goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

  • Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling Cytokeratin 18 with ab189444 at 1/10 dilution followed by Alexa Fluor 488-conjugated Goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

  • Flow cytometry analysis of WiDr cells using ab189444 at 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated Goat-anti-rabbit secondary antibodies were used for the analysis.

References

This product has been referenced in:

  • Gonzalez ME  et al. Mesenchymal Stem Cell-Induced DDR2 Mediates Stromal-Breast Cancer Interactions and Metastasis Growth. Cell Rep 18:1215-1228 (2017). WB ; Human . Read more (PubMed: 28147276) »
  • Xiang Y  et al. Effect of Yi Guan Jian decoction on differentiation of bone marrow mesenchymalstem cells into hepatocyte-like cells in dimethylnitrosamine-induced liver cirrhosis in mice. Mol Med Rep 15:613-626 (2017). Read more (PubMed: 28035356) »
See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab189444.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up